New Method for Measurement of Guanidinosuccinic Acid in Serum and Urine

Gróf, J; Tankó, A; Menyhárt, J

doi:10.1093/clinchem/20.5.574

Cited by 3 publications

(1 citation statement)

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“…Although there has been a considerable improvement in the methods for measuring guanidines, previously published methods (Menichini et al, 1971: Grof et al, 1974Pate1 & Cohen, 1975;Shainkin et al, 1975) have been insufficiently sensitive to establish normal serum values for guanidinosuccinic acid. The measurement of normal serum values for guanidinosucciniC acid was achieved by concentrating the eluate obtained from ion exchange chromatography by evaporation at room temperature and by using the more sensitive fluorimetric assay technique of Conn (1960).…”

Section: Discussionmentioning

confidence: 99%

Concentration of Guanidines in Normal Human Plasma

Lazdins

Dawborn

1978

Clin Exp Pharma Physio

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1. The concentration of methylguanidine and guanidinosuccinic acid has been measured in normal human plasma and discrepancies between various published methods for measuring methylguanidine have been resolved. Previous methods have been modified to allow these guanidines, together with guanidinoacetic acid and creatine, to be measured on the same serum sample. 2. Mean values in six normal subjects were as follows: methylguanidine 1.62 mumol/1 (s.d. = 1.3); guanidinosuccinic acid 0.66 mumol/1 (s.d. = 0.22); guanidinoacetic acid 7.06 mumol/1 (s.d. = 1.29); creatine 80.9 mumol/1 (s.d. = 37.9).

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Section: Discussionmentioning

confidence: 99%

Concentration of Guanidines in Normal Human Plasma

Lazdins

Dawborn

1978

Clin Exp Pharma Physio

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Simultaneous determination of guanidinosuccinic acid and guanidinoacetic acid in urine using high performance liquid chromatography/tandem mass spectrometry

Saigusa

Suzuki

Takahashi

et al. 2010

Analytica Chimica Acta

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Gas—Liquid Chromatographic Measurement of Guanidino Acids

Patel¹,

Cohen²

1975

Clinical Chemistry

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We present a method for separating and measuring guanidino acids by gas—liquid chromatography. Compared to ion-exchange techniques, this system is faster, more sensitive, requires smaller sample volumes, is independent of colorimetric reactions, and permits simultaneous determination of both amino and guanidino acids. N-Trifluoroacetyl-n-butyl esters are formed and then are separated by using a column containing a mixed silicone liquid phase coated on Chromosorb W-HP. Analytical recoveries from plasma ranged from 86 to 112% and were optimal when purification and derivatization were done without prior protein precipitation. Speculations as to the cause of this interference by protein include coprecipitation, protein-binding, and cyclization of the guanidines in the acids used for denaturation. Evidence presented suggests that all three occur: ultrafiltrability is diminished in the presence of protein and nuclear magnetic resonance demonstrates cyclization of some of these esters.

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New Method for Measurement of Guanidinosuccinic Acid in Serum and Urine

Abstract: We describe a new method for measurement of guanidinosuccinate in serum and urine. To separate it from interfering guanidino compounds, it is adsorbed on and eluted from an anion-exchange column. The modified method requires less serum and is considerably faster than previous ones.

Cited by 3 publications

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Concentration of Guanidines in Normal Human Plasma

Concentration of Guanidines in Normal Human Plasma

Simultaneous determination of guanidinosuccinic acid and guanidinoacetic acid in urine using high performance liquid chromatography/tandem mass spectrometry

Gas—Liquid Chromatographic Measurement of Guanidino Acids

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