New method for the detection of reactive oxygen species in anti-tumoural activity of adriamycin: A comparison between hypoxic and normoxic cells

Rharass, Tareck; Vigo, Jean; Salmon, Jean‐Marie; Ribou, Anne‐Cécile

doi:10.1080/10715760701834552

Cited by 14 publications

(13 citation statements)

References 31 publications

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“…In sperm samples taken from males and from females after short (6 h post mating), intermediate (3.5 weeks post mating) and long (10 weeks post mating) duration of storage, we recorded the fluorescence lifetime of an intra-sperm probe (1-pyrene butyric acid, PBA)29. The fluorescence lifetime of this probe decreases with increasing concentration of oxygen radicals17323334. If females would adaptively reduce oxidative damage in the stored sperm cells, we expected higher fluorescence lifetimes in sperm cells that were stored for short or intermediate duration (before the start of infertility) compared to males.…”

Section: Resultsmentioning

confidence: 99%

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Females become infertile as the stored sperm's oxygen radicals increase

Reinhardt

Ribou

2013

Sci Rep

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Predicting infertility is central to reproductive biology, medicine and evolutionary biology. In-vitro studies suggest that oxidative sperm damage causes infertility. Oxidative sperm damage can be reduced via two fundamental pathways: the removal of oxygen radicals by antioxidants, or the interference with cell metabolism to reduce the formation of oxygen radicals. Oxidative damage protection of spermatozoa should evolve frequently, especially during female sperm storage. However, in-vivo evidence linking oxidative protection and fertility is rare. We show that the intra-sperm production rate of oxygen radicals and the sperm metabolic rate were reduced in female bedbugs, Cimex lectularius, compared to males, and females laid fertile eggs. Females became infertile when sperm oxygen radicals and sperm metabolic rate increased to male levels. Our results link female fitness to sublethal sperm damage, imply adaptive benefits of interfering with sperm metabolism and offer the hypothesis that polyandry may serve to replace low-quality sperm.

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Section: Resultsmentioning

confidence: 99%

“…1-pyrene butyric acid (PBA) is a cell permeable oxygen probe sensitive to free radicals in solution33 and living cells3234. Upon collision with PBA, oxygen free radicals change PBA fluorescence intensity and decrease fluorescence lifetime.…”

Section: Methodsmentioning

confidence: 99%

Females become infertile as the stored sperm's oxygen radicals increase

Reinhardt

Ribou

2013

Sci Rep

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“…Hypoxia-induced mechanisms of resistance to doxorubicin are likely to be multifactorial and include reduced drug accumulation and increased drug efflux 40–42, resistance to apoptosis 43, decreased levels of topoisomerase II 44,45 and a reduction in free radical-dependent DNA damage 46. Toxic effects that are oxygen dependent are reduced in hypoxia 47.…”

Section: Discussionmentioning

confidence: 99%

Hypoxia as a target for drug combination therapy of liver cancer

et al. 2017

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Hepatocellular carcinoma (HCC) is the third most frequent cause of cancer deaths worldwide. The standard of care for intermediate HCC is transarterial chemoembolization, which combines tumour embolization with locoregional delivery of the chemotherapeutic doxorubicin. Embolization therapies induce hypoxia, leading to the escape and proliferation of hypoxia-adapted cancer cells. The transcription factor that orchestrates responses to hypoxia is hypoxia-inducible factor 1 (HIF-1). The aim of this work is to show that targeting HIF-1 with combined drug therapy presents an opportunity for improving outcomes for HCC treatment. HepG2 cells were cultured under normoxic and hypoxic conditions exposed to doxorubicin, rapamycin and combinations thereof, and analyzed for viability and the expression of hypoxia-induced HIF-1α in response to these treatments. A pilot study was carried out to evaluate the antitumour effects of these drug combinations delivered from drug-eluting beads in vivo using an ectopic xenograft murine model of HCC. A therapeutic doxorubicin concentration that inhibits the viability of normoxic and hypoxic HepG2 cells and above which hypoxic cells are chemoresistant was identified, together with the lowest effective dose of rapamycin against normoxic and hypoxic HepG2 cells. It was shown that combinations of rapamycin and doxorubicin are more effective than doxorubicin alone. Western Blotting indicated that both doxorubicin and rapamycin inhibit hypoxia-induced accumulation of HIF-1α. Combination treatments were more effective in vivo than either treatment alone. mTOR inhibition can improve outcomes of doxorubicin treatment in HCC.

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“…Oxidative stress is a cytotoxic process in which the balance of the production and elimination of intracellular oxygen free radicals is tipped, resulting in overaccumulation of ROS 14 . Compelling evidence indicates that oxidative stress is a critical trigger in the complex chain of events leading to atherosclerosis, one of the major manifestations of metabolic syndrome.…”

Section: Discussionmentioning

confidence: 99%

Hydrogen Peroxide Enhances Osteopontin Expression and Matrix Metalloproteinase Activity in Aortic Vascular Smooth Muscle Cells

Hu¹,

Luan²,

Zhang³

et al. 2009

Clin Exp Pharma Physio

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1. Restenosis after percutaneous coronary intervention (PCI) is a major clinical complication. However, the underlying mechanisms remain poorly understood. The present aim of the present study was to test the hypothesis that reactive oxygen species (ROS) enhance osteopontin (OPN) expression and increase matrix metalloproteinase (MMP)-2 activity (two major factors that contribute to restenosis) in aortic vascular smooth muscle cells (VSMC), thus facilitating restenosis. 2. Primary cultured rat aortic VSMC were exposed to different concentrations (10, 50 and 100 micromol/L) of H(2)O(2). The expression of OPN mRNA and protein was determined by reverse transcription-polymerase chain reaction and Western blotting, respectively. The activity of MMP-2 was determined by gelatin zymography. 3. The expression of OPN mRNA and protein in VSMC was enhanced by H(2)O(2) in a dose-dependent manner. In addition, H(2)O(2) at all concentrations tested (which are comparable to those seen in diabetic vascular tissues) significantly increased MMP-2 activity in VSMC. 4. Because vascular ROS production is significantly increased in patients with ischaemic disease and OPN and MMP-2 have been shown to play critical role in restenosis, the results of the present study strongly suggest that a ROS-initiated and OPN- and MMP-2-mediated signalling pathway may play an important role in accelerated restenosis after PCI in patients with ischaemic disease. Therefore, the H(2)O(2)-OPN/MMP-2 system may be a new therapeutic target in reducing restenosis in patients undergoing PCI.

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New method for the detection of reactive oxygen species in anti-tumoural activity of adriamycin: A comparison between hypoxic and normoxic cells

Cited by 14 publications

References 31 publications

Females become infertile as the stored sperm's oxygen radicals increase

Females become infertile as the stored sperm's oxygen radicals increase

Hypoxia as a target for drug combination therapy of liver cancer

Hydrogen Peroxide Enhances Osteopontin Expression and Matrix Metalloproteinase Activity in Aortic Vascular Smooth Muscle Cells

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