The 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
DOI: 10.1109/iembs.2004.1404419
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New methods for automated phenotyping of complex cellular behaviors

Abstract: Cellular shape change and movement are central to biologic processes that range from normal embryonic development to inflammatory diseases and cancer. Quantitative visual phenotyping of dynamic cellular behaviors creates unique challenges for image capture, analysis and storage. Despite substantial technological advances in molecular biology, biochemistry, genomics and proteomics, investigating cellular processes remains tremendously challenging and labor-intensive. We have developed algorithms and software im… Show more

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Cited by 3 publications
(7 citation statements)
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“…Such restriction in movement is in agreement with data showing peripheral localization correlates with increased motion constraint in human cells [ 30 ]. This notion is further supported by analysis with the Visible Discovery software [ 29 ], in which the motion of the GAL locus is positively correlated with the movement of the nucleus when induced and at the periphery.…”
Section: Discussionmentioning
confidence: 93%
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“…Such restriction in movement is in agreement with data showing peripheral localization correlates with increased motion constraint in human cells [ 30 ]. This notion is further supported by analysis with the Visible Discovery software [ 29 ], in which the motion of the GAL locus is positively correlated with the movement of the nucleus when induced and at the periphery.…”
Section: Discussionmentioning
confidence: 93%
“…To demonstrate the application of machine visual screening to the study of chromosome dynamics, we took the same image series analyzed by traditional geometric methods above and applied the machine visual screening software, Visible Discovery [ 29 ]. This software generates an aggregate change index (ACI), which is a measure of movement; in this case how spatial organization of fluorescent intensity shifts over time for each frame of the green and red channels.…”
Section: Resultsmentioning
confidence: 99%
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“…Combining these techniques with genome‐wide siRNA knockdown has led to successful screens for novel members of specific pathways, and exploration of the genetic networks responsible for fundamental cellular functions 55,56. The ongoing development of robust reporters for a host of cellular processes in the zebrafish, and their potential implementation in an automated or semiautomated mode, will hopefully open these phenomena to unbiased exploration at scale in the zebrafish 57. The availability of reporters for specific biochemical processes allows these also to be integrated in four‐dimensional maps of organismal biology.…”
Section: Cell Functionmentioning
confidence: 99%
“…Several groups have successfully adapted imaging or other assays to multiwell plates and undertaken screens for genetic or chemical modifiers with varying degrees of success. For example, in the cardiovascular arena, sophisticated assays of cardiac rate, rhythm, contractility, blood flow, vasomotion, and rheology are available, and can often be multiplexed using different fluorescent reporters and refined image analysis techniques 43,45,57. Ca 2+ reporters have been used to screen for mutants affecting the developmental maturation of cardiac physiology.…”
Section: In Vivo Physiology At Scalementioning
confidence: 99%