New microsatellite loci for Prosopis alba and P. chilensis (Fabaceae)

Abstract

• Premise of the study: As only six useful microsatellite loci that exhibit broad cross-amplification are so far available for Prosopis species, it is necessary to develop a larger number of codominant markers for population genetic studies. Simple sequence repeat (SSR) markers obtained for Prosopis species from a 454 pyrosequencing run were optimized and characterized for studies in P. alba and P. chilensis.• Methods and Results: Twelve markers that were successfully amplified showed polymorphism in P. alba and P. chilensis. The number of alleles per locus ranged between two and seven and heterozygosity estimates ranged from 0.2 to 0.8. Most of these loci cross-amplify in P. ruscifolia, P. flexuosa, P. kuntzei, P. glandulosa, and P. pallida.• Conclusions: These loci will enable genetic diversity studies of P. alba and P. chilensis and contribute to fine-scale population structure, indirect estimation of relatedness among individuals, and marker-assisted selection.

Genetic diversity and differentiation among Prosopis alba (Leguminosae) populations from dry valleys of Bolivia with different levels of human disturbance and altitude

“…Total genomic DNA was extracted using the Qiagen DNeasy Plant kit (Qiagen, Valencia, CA) from leaf material that was collected and stored in silica gel. The samples were genotyped at 10 microsatellite markers described by Mottura, Finkeldey, Verga, and Gailing (), Mo05, Mo13, Mo08, Mo09, and Bessega et al (), GL8, GL18, GL24, GL15 GL12, and GL6. The PCR amplifications were carried out in a 50 μL reaction volume containing 10–30 ng DNA, 0.6 μM each primer, 0.2 mM dNTPs, 0.3 U Taq DNA polymerase (Invitrogen, Buenos Aires, Argentina), and 1.5 mM MgCl 2.…”

Genetic diversity and structure of the vulnerable species Prosopis chilensis (Molina) Stuntz in the Coquimbo Region, Chile

“…The quality of the obtained DNA was evaluated on 0.8% agarose gels stained with ethidium bromide and DNA concentration was measured with a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Inc. Wilmington, DE, USA), according to the user's manual. (Mottura et al 2005, Bessega et al 2013, Torales et al 2013, Alves et al 2014, Pomponio et al 2015. This study was based on six nuclear microsatellite loci: Mo05, Mo07, Mo08, Mo09, Mo13, and Mo16, described for P. chilensis by Mottura et al (2005).…”

Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species

“…In the Prosopis genus, SSR markers have been developed through the construction of enriched genomic microsatellite libraries (Mottura et al, 2005;Alves et al, 2014) and, through high generation sequencing techniques either from genomics (Bessega et al, 2013) and transcriptomics (Torales et al, 2013).…”