New models for analyzing mast cell functions in vivo

Reber, Laurent L.; Marichal, Thomas; Galli, Stephen J.

doi:10.1016/j.it.2012.09.008

Cited by 168 publications

(197 citation statements)

References 139 publications

(176 reference statements)

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“…We proposed, based on experiments performed in mice with a profound MC deficiency related to abnormalities in Kit structure or expression (referred to herein as "Kit-mutant MC-deficient mice"), that MCs and MC production of IL-10 can limit skin pathology during severe contact hypersensitivity (CHS) reactions (6). As noted in that study, it has been appreciated for some time that Kit-mutant mice have several phenotypic abnormalities in addition to their MC deficiency, and subsequently we and others generated Kit-independent MC-deficient mouse strains that are deficient in MC populations due to genetic manipulations independent of alterations in Kit (7)(8)(9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Imaging protective mast cells in living mice during severe contact hypersensitivity

Reber¹,

Sibilano²,

Starkl³

et al. 2017

JCI Insight

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Section: Introductionmentioning

confidence: 99%

Imaging protective mast cells in living mice during severe contact hypersensitivity

Reber¹,

Sibilano²,

Starkl³

et al. 2017

JCI Insight

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“…Numerous methodologies have been applied to study biological functions of MCs [9][10][11][12][13] . Many groups have focused on in vitro approaches using either cell lines (such as the human MC lines HMC1 14 or LAD2 15,16 ), in vitro derived MCs (such as human peripheral blood-derived MCs 17 , or mouse bone marrow-derived cultured MCs [BMCMCs] 18 , fetal skin-derived cultured MCs [FSCMCs] 19 and peritoneal cell-derived MCs [PCMCs] 20 ) or ex vivo isolated MCs from different anatomical sites.…”

Section: Introductionmentioning

confidence: 99%

“…However, an important aspect of MCs biology is that their phenotypic and functional characteristics (e.g., cytoplasmic granule protease content or response to different stimuli) can be modulated by anatomical location and microenvironment 2,7 . Since the exact mixture of such factors that are encountered in vivo may be difficult to reproduce in vitro, we favor using in vivo approaches to gain insights into MCs functions 9 .…”

Section: Introductionmentioning

confidence: 99%

“…These mice lack expression and/or activity of KIT (CD117), the receptor for the main MC growth factor stem cell factor (SCF) 21,22 . As a result, these mice have a profound MC deficiency but also have additional phenotypic abnormalities related to their c-kit mutations (in the WBB6F 1 -Kit W/W-v mice) or to the effects of the large chromosomal inversion that results in reduced c-kit expression (in the C57BL/6-Kit W-sh/W-sh mice) 9,10,12,23 . More recently, several strains of mice with c-kit-independent constitutive MC deficiency have been reported [24][25][26] .…”

Section: Introductionmentioning

confidence: 99%

“…More recently, several strains of mice with c-kit-independent constitutive MC deficiency have been reported [24][25][26] . All these mice and some additional new types of inducible MC-deficient mice have been recently reviewed in detail 9,10,13 .…”

Section: Introductionmentioning

confidence: 99%

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Analyzing the Functions of Mast Cells <em>In Vivo</em> Using '<em>Mast Cell Knock-in</em>' Mice

Gaudenzio

Sibilano

Starkl

et al. 2015

JoVE

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Mast cells (MCs) are hematopoietic cells which reside in various tissues, and are especially abundant at sites exposed to the external environment, such as skin, airways and gastrointestinal tract. Best known for their detrimental role in IgE-dependent allergic reactions, MCs have also emerged as important players in host defense against venom and invading bacteria and parasites. MC phenotype and function can be influenced by microenvironmental factors that may differ according to anatomic location and/or based on the type or stage of development of immune responses. For this reason, we and others have favored in vivo approaches over in vitro methods to gain insight into MC functions. Here, we describe methods for the generation of mouse bone marrow-derived cultured MCs (BMCMCs), their adoptive transfer into genetically MC-deficient mice, and the analysis of the numbers and distribution of adoptively transferred MCs at different anatomical sites. This method, named the 'mast cell knock-in' approach, has been extensively used over the past 30 years to assess the functions of MCs and MC-derived products in vivo. We discuss the advantages and limitations of this method, in light of alternative approaches that have been developed in recent years. Video LinkThe video component of this article can be found at

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Basophils

Mukai

2013

Encyclopedia of Life Sciences

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Basophilic granulocytes or ‘basophils’ are a type of leucocyte (‘white blood cell’) that circulates in the blood. They are the rarest of the granulocytes (the others are neutrophils and eosinophils), usually representing less than 0.5% of leucocytes in the peripheral blood of humans or mice. Basophils can participate in the expression of acute and chronic and allergic diseases, including anaphylaxis, asthma, atopic dermatitis and hay fever. Basophils also can contribute to resistance to internal parasites (such as helminths) and ectoparasites (such as ticks). At a steady state, upon leaving the bone marrow, basophils reside mainly in the peripheral blood but can migrate into tissues such as lymph nodes, skin and the lungs where they can play roles in regulating immune responses or in the pathogenesis of diseases. When they participate in allergic reactions or responses to parasites, basophils release histamine and many other biologically active molecules that can contribute to inflammation. Key Concepts: Basophils are the rarest granulocytes circulating in the peripheral blood. Basophils are increased during inflammatory responses such as certain types of skin inflammation, asthma or parasite infections, settings in which basophils may appear in the affected tissues. Basophils are activated when antigen‐specific IgE antibodies that may recognise antigens derived from parasites or from substances that cause allergies (such as ragweed allergens in subjects with hay fever induced by ragweed) that are bound to the basophils' high‐affinity IgE receptors (FcɛRI) encounter bi‐ or multivalent antigen recognised by that FcɛRI‐bound IgE. Basophils activated by IgE and antigen can help to induce the development of acute allergic reactions, such as anaphylaxis to bee stings or peanut products, and also chronic allergic reactions, such as in asthma or atopic dermatitis. Basophils have common features with mast cells (which are normally located in the tissues, but, unlike basophils, are not normally present in the blood), and basophils and mast cells can play similar or overlapping roles, as well as have some distinct roles, in the induction of allergic diseases or in immune responses against parasites or ticks. Chronic myelogenous leukaemia patients often have a markedly increased number of basophils in the blood and bone marrow.

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New models for analyzing mast cell functions in vivo

Cited by 168 publications

References 139 publications

Imaging protective mast cells in living mice during severe contact hypersensitivity

Imaging protective mast cells in living mice during severe contact hypersensitivity

Analyzing the Functions of Mast Cells <em>In Vivo</em> Using '<em>Mast Cell Knock-in</em>' Mice

Basophils

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