New Mouse Model for Studying Laryngeal Transplantation

Shipchandler, Taha Z.; Lott, David G.; Lorenz, Robert R.; Friedman, Aaron D.; Dan, Olivia; Strome, Marshall

doi:10.1177/000348940911800610

Cited by 12 publications

(10 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7 In brief, the laryngotracheal complex was harvested from the C57BL/6 donor mouse. It was then transplanted heterotopically into the C3H recipient mouse.…”

Section: Mouse Laryngeal Transplantationmentioning

confidence: 99%

“…Mice were euthanized at six separate time points (1,3,5,7,9, and 15 days post-transplant). Six mice were euthanized on post-transplant day 1.…”

Section: Data Collectionmentioning

confidence: 99%

“…Our lab recently developed a new mouse laryngeal transplant model to fully evaluate the immune response to various immunosuppressive agents and explore cell-mediated immunosuppression techniques. The model has proven effective and reliable, 7 with syngeneic transplants noted to survive indefinitely. Prior to its use for research purposes, a study of the natural laryngeal allograft rejection process in immunocompetent mice was needed.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A new mouse laryngeal transplantation rejection grading system

et al. 2009

Self Cite

View full text Add to dashboard Cite

Objectives/Hypothesis: Development of a rat laryngeal transplantation model allowed for the first total human laryngeal transplantation by the senior author in 1998. In an effort to further our knowledge of the immune system's role in laryngeal rejection, a change to the mouse model was required. Prior to initiating immunosuppressive research protocols, a reliable mouse larynx rejection classification had to be established.Study Design: Animal study. Methods: Thirty-one mouse laryngeal transplants (C57 BL/6 donors to C3H recipients) were performed and allowed to reject. Six time points were evaluated histologically: 1, 3, 5, 7, 9, and 15 days post-transplant. Eight anatomic sites were evaluated and assigned a point value. A linear regression model was constructed using the group number as the response and the scores from the eight histological criteria as predictors. Severity classifications were determined by observing patterns in the sum of scores of variables found to be significant contributors. Group 1 was normal; group 2, minimal rejection; groups 3, 4, and 5, moderate rejection; and group 6, severe rejection.Results: All mice survived the transplants. Of the observed histological changes, cartilage, fat, muscle, and magnitude of lymphocytic infiltration significantly correlated with rejection severity. The rejection model created demonstrated 100% accuracy in predicting the severity classification for the 31 specimens in the study. Conclusions:The model established provides an accurate and reliable way to classify rejection severity in mice receiving laryngeal allografts. This sets the stage for future advanced study of manipulating the immune system as a mechanism for establishing allograft tolerance.

show abstract

“…7 In brief, the laryngotracheal complex was harvested from the C57BL/6 donor mouse. It was then transplanted heterotopically into the C3H recipient mouse.…”

Section: Mouse Laryngeal Transplantationmentioning

confidence: 99%

“…Mice were euthanized at six separate time points (1,3,5,7,9, and 15 days post-transplant). Six mice were euthanized on post-transplant day 1.…”

Section: Data Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A new mouse laryngeal transplantation rejection grading system

et al. 2009

Self Cite

View full text Add to dashboard Cite

show abstract

“…1). 13 In brief, the laryngotracheal complex was harvested from the C57BL/6 donor mouse. It was then transplanted heterotopically into the C3H recipient mouse.…”

Section: Methodsmentioning

confidence: 99%

Decoy NF‐κB fortified immature dendritic cells maintain laryngeal allograft integrity and provide enhancement of regulatory T cells

et al. 2009

Self Cite

View full text Add to dashboard Cite

Objectives/Hypothesis: The increased risk of malignancy associated with post-transplant immunosuppression limits the potential of laryngeal transplantation as a reconstructive option. This risk may be mitigated by utilizing decoy nuclear factor kappa B (NF-jB) immature dendritic cells (iDC) to provide donor-specific tolerance. The purpose of this study was to explore whether tolerogenic properties of iDC can be applied to composite tissue transplantation.Study Design: Animal study. Methods: Five iDC-injected mice were euthanized at 15, 30, and 60 days post-laryngeal transplant. Control groups included five transplanted mice without immunosuppression, one iDC-injected mouse euthanized prior to transplantation, one mouse without injection or transplantation, and one mouse administered mature DC to serve as an accelerated rejection control. Larynges were graded for rejection severity according to a grading scale. Draining lymph nodes and spleens were evaluated by flow cytometry to determine immunogenic activity of iDC and T cells locally and peripherally.Results: Each time group demonstrated moderate allograft rejection (rejection severity scores: 4.38, 5.10, 5.29). NF-jB iDC-treated mice had significantly less rejection at all time points compared to nonimmunosuppressed mice. Flow cytometry showed inhibition of cytotoxic T cell infiltration and expansion of regulatory T cells at the allograft site.Conclusions: iDC immunosuppression maintains laryngeal allograft integrity up to 60 days posttransplantation. Regulatory T cells are enhanced at the allograft site, which serves to suppress immune cell activation and induce self-antigen tolerance. iDC injection may lessen post-transplant comorbidities by decreasing the systemic immune response and favorably affecting concurrent immunosuppressive dose sequencing for laryngeal allograft preservation.

show abstract

“…Another cervical heterotopic transplantation model in mice is the heterotopic laryngeal transplantation model by Shipchandler et al [23]. The authors developed a laryngeal graft based on a vascular pedicle composed of bilateral common carotid artery and its thyroid branches.…”

Section: Mouse Vca Modelsmentioning

confidence: 99%

Mouse Models of Experimental Vascularized Composite Allotransplantation

Moine

2014

Curr Transpl Rep

View full text Add to dashboard Cite

The clinical practice of vascularized composite allotransplantation (VCA) has been limited by the potential side effects of chronic immunosuppression. Studies using rodent models have been useful for dissecting mechanisms underlying immunological events induced by VCA and for developing protocols such as mixed chimerism for tolerance induction. Mouse models of VCA have great advantages over rat and other rodents with regard to dissection of immunologic mechanisms; however, the microsurgical revascularization procedures that are required are much more difficult. Here we review recent advances in surgical and immunological methods for successful VCA in the mouse model.

show abstract

New Mouse Model for Studying Laryngeal Transplantation

Abstract: This pilot study describes the reproducible surgical techniques performed in using mice for studying laryngeal transplantation. Mice are cost-effective and immunologically well studied, and are thus ideal for further laryngeal transplantation research.

Cited by 12 publications

References 7 publications

A new mouse laryngeal transplantation rejection grading system

A new mouse laryngeal transplantation rejection grading system

Decoy NF‐κB fortified immature dendritic cells maintain laryngeal allograft integrity and provide enhancement of regulatory T cells

Mouse Models of Experimental Vascularized Composite Allotransplantation

Contact Info

Product

Resources

About