New mouse model of skeletal muscle atrophy using spiral wire immobilization

Onda, Akiko; Kono, Hajime; Jiao, Qibin; Akimoto, Takayuki; Miyamoto, Terumasa; Sawada, Yasuhiro; Kusakari, Yoichiro; Minamisawa, Susumu; Fukubayashi, Toru

doi:10.1002/mus.25202

Cited by 22 publications

(26 citation statements)

References 9 publications

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“…Decrease in muscle strength, loss in muscle mass, and reduction in muscle fibers are common during disuse time (Jaspers and Tischler, 1984). In addition, the activation of UPS, the main regulator of muscle degradation, has been reported in immobilization models (Onda et al, 2016). Other key processes such as inflammation and apoptosis are also known to be activated during inactivity period (Hunter et al, 2002;Yoshihara et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Mice from IV and ID groups were subjected to SWI model according to the Akiko Onda method (Onda et al, 2016). Mice were anesthetized with isoflurane (AbbVie, Berkshire, UK) and maintained in the supine position.…”

Section: Immobilization Proceduresmentioning

confidence: 99%

“…In general, animals subjected to numerous disuse models such as immobilization, hindlimb suspension, and denervation were shown to exhibit similar atrophic muscle characteristics, including muscle weakness associated with reduced muscle fiber size and enhanced muscle protein degradation (Jaspers and Tischler, 1984). For instance, a study with spiral wire immobilization (SWI) model reported 50% lower soleus muscle weight along with the induction of protein degradation markers after 10 days (Onda et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Preventive Effects of Dulaglutide on Disuse Muscle Atrophy Through Inhibition of Inflammation and Apoptosis by Induction of Hsp72 Expression

2020

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Pathological conditions such as joint immobilization, long-time bed rest, or inactivity may result in disuse-induced muscle wasting and dysfunction. To investigate the effect of dulaglutide, a long-acting glucagon-like peptide-1 receptor agonist, on disuse muscle atrophy, disuse condition was induced by spiral wire immobilization in C57BL/6 mice and the mice were treated with dulaglutide. Dulaglutide treatment effectively improved muscle function and increased muscle mass compared with vehicle treatment. Dulaglutide inhibited the decrease of muscle fiber size and the expression of atrophic factors such as myostatin, atrogin-1/MAFbx, and muscle RING-finger protein-1 in immobilized mice. In addition, dulaglutide inhibited nuclear factor kappa B activation, leading to a decrease in the mRNA levels of proinflammatory cytokines, including tumor necrosis factor-a, interleukin (IL)-1b, and IL-6 in muscle of immobilized mice. Dulaglutide suppressed the expression of apoptotic markers such as caspase-3, cleaved poly-ADP ribose polymerase, and Bax under immobilization condition and increased the expression of heat shock protein 72 (Hsp72), which is related to the amelioration of inflammation and apoptosis during disuse time. Further study showed that dulaglutide could induce Hsp72 expression via the regulation of 5′-AMP-activated protein kinase signaling. Our data suggest that dulaglutide could exert beneficial effects against disuse-induced muscle atrophy.

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Section: Discussionmentioning

confidence: 99%

Section: Immobilization Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Preventive Effects of Dulaglutide on Disuse Muscle Atrophy Through Inhibition of Inflammation and Apoptosis by Induction of Hsp72 Expression

2020

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“…Bilateral hindlimbs of mice were immobilized as described previously [23]. Surgical tape (3M Japan, Tokyo, Japan) and aluminum wire (Eko Metal, Niigata, Japan) were applied to mice under hypnosis with sodium pentobarbital (50 mg/kg i.p.)…”

Section: Immobilization Of Mouse Bilateral Hindlimbsmentioning

confidence: 99%

“…We first examined whether mouse hindlimb immobilization by spiral wiring, an animal model of disuse muscle atrophy that we previously developed [23], induced local inflammatory responses. Consistent with our previous observations, both the muscle mass of triceps surae and the CSA of gastrocnemius myofibers were significantly decreased by hindlimb immobilization (Figure 1B-D).…”

Section: Mouse Hindlimb Immobilization By Spiral Wiring Induces Atropmentioning

confidence: 99%

Local cyclical compression modulates macrophage functionin situand alleviates immobilization-induced muscle atrophy

et al. 2018

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Physical inactivity gives rise to numerous diseases and organismal dysfunctions, particularly those related to aging. Musculoskeletal disorders including muscle atrophy, which can result from a sedentary lifestyle, aggravate locomotive malfunction and evoke a vicious circle leading to severe functional disruptions of vital organs such as the brain and cardiovascular system. Although the significance of physical activity is evident, molecular mechanisms behind its beneficial effects are poorly understood. Here, we show that massage-like mechanical interventions modulate immobilization-induced pro-inflammatory responses of macrophages and alleviate muscle atrophy. Local cyclical compression (LCC) on mouse calves, which generates intramuscular pressure waves with amplitude of 50 mmHg, partially restores the myofiber thickness and contracting forces of calf muscles that are decreased by hindlimb immobilization. LCC tempers the increase in the number of cells expressing pro-inflammatory proteins, tumor necrosis factor-α and monocyte chemoattractant protein-1 (MCP-1), including macrophages The reversing effect of LCC on immobilization-induced thinning of myofibers is almost completely nullified when macrophages recruited from circulating blood are depleted by administration of clodronate liposomes. Furthermore, application of pulsatile fluid shear stress, but not hydrostatic pressure, reduces the expression of MCP-1 in macrophages Together with the LCC-induced movement of intramuscular interstitial fluid detected by µCT analysis, these results suggest that mechanical modulation of macrophage function is involved in physical inactivity-induced muscle atrophy and inflammation. Our findings uncover the implication of mechanosensory function of macrophages in disuse muscle atrophy, thereby opening a new path to develop a novel therapeutic strategy utilizing mechanical interventions.

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Engineered Circular RNA CircmiR‐29b Attenuates Muscle Atrophy by Sponging MiR‐29b

Jin

Chen

Zheng

et al. 2022

Advanced Therapeutics

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Muscle atrophy is commonly caused by various diseases but still lacks effective treatment in clinical practice. Here, an artificial circular RNA (circRNA) named circmiR‐29b, which is designed to be a molecular sponge for miR‐29b containing 12 imperfect bulged miR‐29b binding sites is constructed. CircmiR‐29b shows a favorable functional effect with respect to miR‐29b repression in a specific and drastic manner. CircmiR‐29b can protect against in vitro muscle atrophy induced by dexamethasone (Dex), angiotensin II (Ang II), and tumor necrosis factor alpha (TNF‐α). Besides, circmiR‐29b attenuates in vivo muscle atrophy induced by denervation, immobilization, and Ang II. More importantly, skeletal muscle specific gene therapy using circmiR‐29b is able to attenuate established muscle atrophy induced by spiral wire immobilization. This work has developed an engineered circmiR‐29b acting as an miR‐29b sponge, and offers a promising therapeutic tool to prevent muscle atrophy.

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New mouse model of skeletal muscle atrophy using spiral wire immobilization

Abstract: This method allows repeated, direct access to the immobilized muscle, making it a useful procedure for concurrent application and assessment of various therapeutic interventions. Muscle Nerve 54: 788-791, 2016.

Cited by 22 publications

References 9 publications

Preventive Effects of Dulaglutide on Disuse Muscle Atrophy Through Inhibition of Inflammation and Apoptosis by Induction of Hsp72 Expression

Preventive Effects of Dulaglutide on Disuse Muscle Atrophy Through Inhibition of Inflammation and Apoptosis by Induction of Hsp72 Expression

Local cyclical compression modulates macrophage functionin situand alleviates immobilization-induced muscle atrophy

Engineered Circular RNA CircmiR‐29b Attenuates Muscle Atrophy by Sponging MiR‐29b

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