New mRNA isoform of Oct-1 transcription factor is transcribed from alternative promoter

Крылова, И. Д.; Portseva, T. N.; Георгиева, С. Г.; Степченко, А. Г.; Pankratova, E. V.

doi:10.1134/s0026893313040080

Cited by 9 publications

(4 citation statements)

References 15 publications

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“…Oct-1L and Oct-1X do not have an effect on the common target genes opposite to that of Oct-1A, but rather modulate the rate of their activation or repression. It correlates well with the previously obtained data demonstrating that Oct-1 isoforms elicit a varying level of activation of the same target genes in vitro (29,30). …”

Section: Discussionsupporting

confidence: 92%

“…The Oct-1A transcript (NM_002697) contains an open reading frame for Oct-1A protein (766 aa, UniProt P14859-6) which represents the longest form of Oct-1 comprising all the internal exons (30). The Oct-1L (AY113189) (755 aa, UniProt P14859-2) transcript is tissue-specific (31) while Oct-1X mRNA, which we described earlier (KT438684.1) is expressed at a low level in most tissues (29). Still, the presence of specific Oct-1 protein isoforms in mammalian tissues was not investigated and their functions are unknown.…”

Section: Introductionmentioning

confidence: 83%

“…Indeed, 15 alternative human Oct-1 (POU2F1) transcripts are annotated in The Genome Annotation for Alternative Splicing Database (http://genome.ewha.ac.kr/ECgene). Previously, we studied Oct-1A, Oct-1L, and Oct-1X and have demonstrated that they start from alternative promoters of the POU2F1 gene and the predicted isoforms differ by their N-termini (27–29). At present time, the ‘canonical’ Oct-1 protein is considered to be a sequence of 743 aa corresponding to Oct-1X (UniProt, P14859-1).…”

Section: Introductionmentioning

confidence: 99%

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Different N-terminal isoforms of Oct-1 control expression of distinct sets of genes and their high levels in Namalwa Burkitt's lymphoma cells affect a wide range of cellular processes

et al. 2016

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Oct-1 transcription factor has various functions in gene regulation. Its expression level is increased in several types of cancer and is associated with poor survival prognosis. Here we identified distinct Oct-1 protein isoforms in human cells and compared gene expression patterns and functions for Oct-1A, Oct-1L, and Oct-1X isoforms that differ by their N-terminal sequences. The longest isoform, Oct-1A, is abundantly expressed and is the main Oct-1 isoform in most of human tissues. The Oct-1L and the weakly expressed Oct-1X regulate the majority of Oct-1A targets as well as additional sets of genes. Oct-1X controls genes involved in DNA replication, DNA repair, RNA processing, and cellular response to stress. The high level of Oct-1 isoforms upregulates genes related to cell cycle progression and activates proliferation both in Namalwa Burkitt's lymphoma cells and primary human fibroblasts. It downregulates expression of genes related to antigen processing and presentation, cytokine-cytokine receptor interaction, oxidative metabolism, and cell adhesion, thus facilitating pro-oncogenic processes.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

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Different N-terminal isoforms of Oct-1 control expression of distinct sets of genes and their high levels in Namalwa Burkitt's lymphoma cells affect a wide range of cellular processes

et al. 2016

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“…OCT1, located in 1q24.2, is one of the first discovered transcription factors (TFs) of POU orthologous family, and it not only regulates levels of housekeeping genes as histone H2B and tissue-specific gene as B29 [ 23 ] but also harbors the capacity of pro-proliferative function in tumors [ 24 ]. Although the OCT1 transcription factor was found to be over-expressed in many cancer cells [ 21 , 25 ] and there exist considerable investigations of its role in tumors [ 14 – 18 ], in CRCs, the bio-functional and prognostic value of OCT1 has rarely been expounded.…”

Section: Discussionmentioning

confidence: 99%

Elevated OCT1 participates in colon tumorigenesis and independently predicts poor prognoses of colorectal cancer patients

et al. 2015

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Octamer transcription factor 1 (OCT1) was found to influence the genesis and progression of numerous cancers except for colorectal cancer (CRC). This study tried to explore the role of OCT1 in CRC and clarify the association between its expression and patients’ clinical outcome. Transcriptional and post-transcriptional expression of OCT1 was detected in CRC cancerous tissues and paired normal mucosae by real-time PCR as well as immunohistochemistry. Moreover, the effect of OCT1 knockdown on CRC cell proliferation was investigated both in vitro and in vivo using Cell Counting Kit-8 assay, colony-forming assay, and mouse tumorigenicity assay. Expression of OCT1 was found to be elevated in CRC. Suppression of OCT1 significantly inhibited CRC cell proliferation both in vitro and in vivo. Furthermore, upregulated level of OCT1 was significantly associated with N stage, M stage, and American Joint Committee on Cancer (AJCC) stage (P = 0.027, 0.014, and 0.002, respectively) as well as differential degree (P = 0.022). By using multivariate Cox hazard model, OCT1 was also shown to be a factor independently predicting overall survival (OS; P = 0.013, hazard ratio = 2.747, 95 % confidence interval 1.125 to 3.715) and disease-free survival (DFS; P = 0.004, hazard ratio = 2.756, 95 % confidence interval 1.191 to 4.589) for CRC patients. Our data indicate that OCT1 carries weight in colorectal carcinogenesis and functions as a novel prognostic indicator and a promising target of anti-cancer therapy for CRC.

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Reduced Expression of the Tissue-Specific Oct-1L Isoform Exerts an Antitumor Effect on Namalwa Burkitt’s Lymphoma Cells

Portseva

Котнова²,

Bulavkina³

et al. 2022

Mol Biol

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New mRNA isoform of Oct-1 transcription factor is transcribed from alternative promoter

Cited by 9 publications

References 15 publications

Different N-terminal isoforms of Oct-1 control expression of distinct sets of genes and their high levels in Namalwa Burkitt's lymphoma cells affect a wide range of cellular processes

Different N-terminal isoforms of Oct-1 control expression of distinct sets of genes and their high levels in Namalwa Burkitt's lymphoma cells affect a wide range of cellular processes

Elevated OCT1 participates in colon tumorigenesis and independently predicts poor prognoses of colorectal cancer patients

Reduced Expression of the Tissue-Specific Oct-1L Isoform Exerts an Antitumor Effect on Namalwa Burkitt’s Lymphoma Cells

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