New Multi-Walled carbon nanotube of industrial interest induce cell death in murine fibroblast cells

Godoy, Krissia Franco de; Rodolpho, Joice Margareth de Almeida; Brassolatti, Patrícia; Fragelli, Bruna Dias de Lima; Castro, Cynthia Aparecida de; Assis, Marcelo; Cancino-Bernardi, Juliana; Correia, Ricardo de Oliveira; Albuquerque, Yulli Roxenne; Speglich, Carlos; Longo, E.; Aníbal, Fernanda de Freitas

doi:10.1080/15376516.2021.1930311

Cited by 8 publications

(5 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, after washing with water, the size remains much larger than the initial size in water, obtaining values of 565 ± 38 and 624 ± 2 2 nm. This high stability size increases the evidence of the sample particles aggregation due to the formation of corona, which agrees with the results obtained in previous work [15]. This excessive aggregation due to corona formation can significantly alter the physicochemical properties of the particles, in addition to altering their behavior in a biological environment [16].…”

Section: Resultssupporting

confidence: 90%

“…Cell morphology images also visibly show a decrease in the number of cells in the highest concentrations of OCNT-TEPA. These results corroborate studies that used the same OCNT-TEPA nanoparticle in murine fibroblast cells (LA-9) [15].…”

Section: Discussionsupporting

confidence: 88%

“…Data from cell death analyses of the present study contribute to a better understanding of the results discussed to date, since experiments using apoptosis markers reveal that exposure of the nanoparticle OCNT-TEPA in 24 and 48 hours resulted in apoptosis of macrophages J774 A.1. This result presents the same pattern of cell death analyses performed with MWCNT OCNT-TEPA in murine fibroblast cells (LA-9), which demonstrated incidence of initial apoptosis at the first moment (24 hours of exposure), progressing to late apoptosis or possible cellular necrosis in longer periods of exposure, or higher concentrations of OCNT-TEPA, due to the fact that these concentrations present nanoparticle aggregates that can alter mechanisms of cell death through membrane damage [15,45].…”

Section: Discussionsupporting

confidence: 73%

See 2 more Smart Citations

Cytotoxic Effects Caused by Functionalized Carbon Nanotube in Murine Macrophages.

Godoy

Rodolpho

Fragelli

et al. 2022

Cell Physiol Biochem

View full text Add to dashboard Cite

BACKGROUND/AIMS: The development of new nanomaterials has been growing in recent decades to bring benefits in several areas, especially carbon-based nanoparticles, which have unique physical-chemical properties and allow to take on several applications. Consequently, the use of new nanomaterials without previous toxicological studies raises concern about possible harmful health effects. The aim of this study was to investigate the cytotoxic profile of a new multi-walled carbon nanotube (MWCNT) functionalized with tetraethylenepentamine called OCNT-TEPA using in vitro assays in murine macrophage cells linage J774 A.1. METHODS: OCNT-TEPA was characterized by transmission electron microscopy (TEM) and high resolution TEM (HR-TEM), scanning electron microscopy (SEM), zeta potential and dynamic light scattering (DLS), and its cytotoxic effects were evaluated at 24 and 48 hours by cell viability assays (MTT and NR), morphology and cell recovery (optic microscopy and clonogenic assay), formation of reactive oxygen (ROS) and nitric oxide (NO) species, inflammatory profile (IL-6 and TNF cytokines), mitochondrial membrane potential analysis (MMP), activation of the caspase 3 pathway and cell death (flow cytometry). RESULTS: The data showed a significant decrease in cell viability, increased production of ROS and NO, alteration of mitochondrial membrane potential, increased levels of inflammatory cytokines, alteration of cell morphology, activation of the Caspase 3 pathway and consequently cell death, in the highest concentrations of OCNT-TEPA tested in the periods of 24 and 48 hours. CONCLUSION: The analyses showed that OCNT-TEPA has a dose-dependent cytotoxic profile, which may be harmful to murine macrophages (J774 A.1) and may represent a health risk.

show abstract

Section: Resultssupporting

confidence: 90%

Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 73%

See 1 more Smart Citation

Cytotoxic Effects Caused by Functionalized Carbon Nanotube in Murine Macrophages.

Godoy

Rodolpho

Fragelli

et al. 2022

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…The biological behavior of nanoparticles is determined by their characteristics, as their size can interfere with how they act or do not act on the cell surface, exposing them to cytotoxic risks. Many authors have reported that toxicity may be related to the size of the material associated with nanoparticles and a greater understanding these interactions and their toxicological impact is essential [ 18 , 19 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

Carbon Black CB-EDA Nanoparticles in Macrophages: Changes in the Oxidative Stress Pathway and in Apoptosis Signaling

et al. 2023

Self Cite

View full text Add to dashboard Cite

The influence of black carbon nanoparticles on J774.A1 murine cells was investigated with the objective of exploring the cytotoxicity of black carbon functionalized with ethylenediamine CB-EDA. The results showed that CB-EDA has a cytotoxic profile for J774.A1 macrophages in a time- and dose-dependent manner. When phagocytosed by the macrophage, CB-EDA triggers a mechanism that leads to apoptosis. In this process, there is an increase in oxidative stress pathways due to the activation of nitric oxide and then ROS. This causes an imbalance in redox function and a disruption of membrane integrity that occurs due to high levels of LDH, in addition to favoring the release of the pro-inflammatory cytokines IL-6, IL-12, and tumor necrosis factor (TNF) in an attempt to modulate the cell. However, these stimuli are not sufficient to repair the cell and the level of mitochondrial integrity is affected, causing a decrease in cell viability. This mechanism may be correlated with the activation of the caspasse-3 pathway, which, when compromised, cleaves and induces cells death via apoptosis, either through early or late apoptosis. In view of this, the potential for cell damage was investigated by analyzing the oxidative and inflammatory profile in the macrophage lineage J774.A1 and identifying potential mechanisms and metabolic pathways connected to these processes when cells were exposed to NP CB-EDA for both 24 h and 48 h.

show abstract

“…In vitro and in vivo toxicological studies have shown that MWCNTs have the potential to induce inflammatory cytokine and chemokine production. de Godoy et al have conducted cytotoxicity and cell proliferation tests and inflammatory cytokine assay (TNF-α), and the results revealed a decrement in cell viability and increment of TNF-α secretion with increased intracellular ROS production, indicating an inflammatory profile. Another study has performed RNA sequencing of the macrophage-like cell line after MWCNT exposure for 6, 12, and 24 h, and the downstream analysis of transcriptomics data has noted a significant effect of MWCNT on several chemokines, such as CCL17 and CCL22, which have been implicated in type 2 immune responses and may play a key role in asthma and in atopic dermatitis …”

Section: Potential Health Risksmentioning

confidence: 99%

Functional Micro-/Nanostructures in Agrofood Science: Precise Inspection, Hazard Elimination, and Potential Health Risks

Liu

Wang

Yao

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

Nanotechnology, biotechniques, and chemical engineering have arisen as new trends with significant impacts on agrofood science development. Advanced analytical techniques with high sensitivity, specificity, and automation based on micro-/ nanomaterials for food hazard elimination have become leading research hotspots in agrofood science. Research progress in micro-/ nanomaterials has provided a solid theoretical basis and technical support to solve problems in the industry. However, the rapid development of micro-/nanostructures has also raised concerns regarding potential risks to human health. This review presents the latest advances in the precise inspection and elimination of food hazards from micro-/nanomaterials and discusses the potential threats to human health posed by nanomaterials. The theoretical reference was provided for the application trend of micro-/ nanomaterials in the field of agrofood science in the future.

show abstract

New Multi-Walled carbon nanotube of industrial interest induce cell death in murine fibroblast cells

Cited by 8 publications

References 75 publications

Cytotoxic Effects Caused by Functionalized Carbon Nanotube in Murine Macrophages.

Cytotoxic Effects Caused by Functionalized Carbon Nanotube in Murine Macrophages.

Carbon Black CB-EDA Nanoparticles in Macrophages: Changes in the Oxidative Stress Pathway and in Apoptosis Signaling

Functional Micro-/Nanostructures in Agrofood Science: Precise Inspection, Hazard Elimination, and Potential Health Risks

Contact Info

Product

Resources

About