New Nanotechnologies for the Treatment and Repair of Skin Burns Infections

Souto, Eliana B.; Ribeiro, André F; Ferreira, Maria Inês; Teixeira, M.C.; Shimojo, Andréa Arruda Martins; Soriano, José L; Naveros, Beatriz Clarés; Durazzo, Alessandra; Lucarini, Massimo; Souto, Selma B.; Santini, Antonello

doi:10.3390/ijms21020393

Cited by 102 publications

(59 citation statements)

References 108 publications

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“…The ratio 80:20 exhibited higher consistency, suitable for topical application. The dressings should be flexible enough to allow adherence to the skin/tissue to be treated for a prolonged period, offering more comfort and convenience to the patient [32]. The formation of strong intermolecular interactions between sodium alginate and positively charged polymers is responsible for the increased consistency, including hydrogen bonds and electrostatic attractions that occur at higher concentrations of both polymers [7,28].…”

Section: Resultsmentioning

confidence: 99%

Silver Nanoparticles-Composing Alginate/Gelatine Hydrogel Improves Wound Healing In Vivo

et al. 2020

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Polymer hydrogels have been suggested as dressing materials for the treatment of cutaneous wounds and tissue revitalization. In this work, we report the development of a hydrogel composed of natural polymers (sodium alginate and gelatin) and silver nanoparticles (AgNPs) with recognized antimicrobial activity for healing cutaneous lesions. For the development of the hydrogel, different ratios of sodium alginate and gelatin have been tested, while different concentrations of AgNO3 precursor (1.0, 2.0, and 4.0 mM) were assayed for the production of AgNPs. The obtained AgNPs exhibited a characteristic peak between 430–450 nm in the ultraviolet-visible (UV–Vis) spectrum suggesting a spheroidal form, which was confirmed by Transmission Electron Microscopy (TEM). Fourier Transform Infra-red (FT–IR) analysis suggested the formation of strong intermolecular interactions as hydrogen bonds and electrostatic attractions between polymers, showing bands at 2920, 2852, 1500, and 1640 cm−1. Significant bactericidal activity was observed for the hydrogel, with a Minimum Inhibitory Concentration (MIC) of 0.50 µg/mL against Pseudomonas aeruginosa and 53.0 µg/mL against Staphylococcus aureus. AgNPs were shown to be non-cytotoxic against fibroblast cells. The in vivo studies in female Wister rats confirmed the capacity of the AgNP-loaded hydrogels to reduce the wound size compared to uncoated injuries promoting histological changes in the healing tissue over the time course of wound healing, as in earlier development and maturation of granulation tissue. The developed hydrogel with AgNPs has healing potential for clinical applications.

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Section: Resultsmentioning

confidence: 99%

Silver Nanoparticles-Composing Alginate/Gelatine Hydrogel Improves Wound Healing In Vivo

et al. 2020

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“…The first route for dermal penetration involves transport through the stratum corneum, either by transcellular (diffusion through the cells) or intercellular pathway (diffusion through the gaps between corneocytes). The second route for epidermal penetration includes the entry via skin appendixes as hair follicles and sweat glands [87].…”

Section: Cutaneous Absorptionmentioning

confidence: 99%

Metal-Based Nanoparticles as Antimicrobial Agents: An Overview

et al. 2020

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Metal-based nanoparticles have been extensively investigated for a set of biomedical applications. According to the World Health Organization, in addition to their reduced size and selectivity for bacteria, metal-based nanoparticles have also proved to be effective against pathogens listed as a priority. Metal-based nanoparticles are known to have non-specific bacterial toxicity mechanisms (they do not bind to a specific receptor in the bacterial cell) which not only makes the development of resistance by bacteria difficult, but also broadens the spectrum of antibacterial activity. As a result, a large majority of metal-based nanoparticles efficacy studies performed so far have shown promising results in both Gram-positive and Gram-negative bacteria. The aim of this review has been a comprehensive discussion of the state of the art on the use of the most relevant types of metal nanoparticles employed as antimicrobial agents. A special emphasis to silver nanoparticles is given, while others (e.g., gold, zinc oxide, copper, and copper oxide nanoparticles) commonly used in antibiotherapy are also reviewed. The novelty of this review relies on the comparative discussion of the different types of metal nanoparticles, their production methods, physicochemical characterization, and pharmacokinetics together with the toxicological risk encountered with the use of different types of nanoparticles as antimicrobial agents. Their added-value in the development of alternative, more effective antibiotics against multi-resistant Gram-negative bacteria has been highlighted.

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“…The small-sized particles improve cell uptake and subcellular traffic, and ensure the therapeutic action of essential oil at extracellular and intracellular levels. Topical administration of essential oils is commonly reported for wound healing, hydration, elasticity, scar treatment, and rejuvenation [35]. Their loading into SLNs improves the fixation of the essential oil on the skin, facilitating its penetration.…”

Section: Introductionmentioning

confidence: 99%

(+)-Limonene 1,2-Epoxide-Loaded SLNs: Evaluation of Drug Release, Antioxidant Activity, and Cytotoxicity in an HaCaT Cell Line

Souto

Zielińska

Souto

et al. 2020

IJMS

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In this work, we developed a solid lipid nanoparticle (SLN) formulation with (+)-limonene 1,2-epoxide and glycerol monostearate (Lim-SLNs), stabilized with Poloxamer® 188 in aqueous dispersion to modify the release profile of the loaded monoterpene derivative. We also evaluated the role of SLNs in lipid peroxidation and cytotoxicity in a spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin (the HaCaT cell line). For the cell viability assay, the colorimetric 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used. Lim-SLNs with a loading capacity and encapsulation efficiency of 0.39% and 63%, respectively, were produced by high pressure homogenization. A mean particle size of 194 ± 3.4 nm and polydispersity index of 0.244 were recorded for the loaded Lim-SLNs, as compared to 203 ± 1.5 nm (PI 0.213) for the non-loaded (blank) SLNs. The loading of the monoterpene derivative into glycerol monostearate SLNs fitted into the zero-order kinetics, and ameliorated both lipid peroxidation and cytotoxicity in a keratinocyte cell line. A promising formulation for antioxidant and anti-tumoral activities is here proposed.

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New Nanotechnologies for the Treatment and Repair of Skin Burns Infections

Cited by 102 publications

References 108 publications

Silver Nanoparticles-Composing Alginate/Gelatine Hydrogel Improves Wound Healing In Vivo

Silver Nanoparticles-Composing Alginate/Gelatine Hydrogel Improves Wound Healing In Vivo

Metal-Based Nanoparticles as Antimicrobial Agents: An Overview

(+)-Limonene 1,2-Epoxide-Loaded SLNs: Evaluation of Drug Release, Antioxidant Activity, and Cytotoxicity in an HaCaT Cell Line

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