New Near-Infrared Fluorescent Probes with Single-Photon Anti-Stokes-Shift Fluorescence for Sensitive Determination of pH Variances in Lysosomes with a Double-Checked Capability

Chen, Tzu-Ho; Zhang, Shuwei; Jaishi, Meghnath; Adhikari, Rashmi; Bi, Jianheng; Fang, Mingxi; Xia, Shuai; Zhang, Yibin; Luck, Rudy L.; Pati, Ranjit; Lee, Hsien-Ming; Luo, Fen‐Tair; Tiwari, Ashutosh; Liu, Haiying

doi:10.1021/acsabm.8b00020

Cited by 40 publications

(19 citation statements)

References 45 publications

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“…In order to test this hypothesis, we conducted colocalization experiments by using commercial Lysosensor Green to determine the intracellular location of probe A . Intracelluar pH values were adjusted by incubating HeLa cells in different pH buffers containing 10 µM nigericine, H + ionphore, which is employed to promote equilibration between intracellular and extracellular pH values [ 7 , 9 , 29 , 41 , 42 ]. Probe A responds sensitively to intracellular pH decreases from 7.01 to 3.50 with gradual fluorescence enhancement due to acid-activated spirolactam ring opening with significantly enhanced π-conjugation, Figure 12 .…”

Section: Resultsmentioning

confidence: 99%

A Near-Infrared Fluorescent Probe Based on a FRET Rhodamine Donor Linked to a Cyanine Acceptor for Sensitive Detection of Intracellular pH Alternations

Zhang

Xia

et al. 2018

Molecules

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A fluorescence resonance energy transfer (FRET)-based near-infrared fluorescent probe (B+) for double-checked sensitive detection of intracellular pH changes has been synthesized by binding a near-infrared rhodamine donor to a near-infrared cyanine acceptor through robust C-N bonds via a nucleophilic substitution reaction. To demonstrate the double-checked advantages of probe B+, a near-infrared probe (A) was also prepared by modification of a near-infrared rhodamine dye with ethylenediamine to produce a closed spirolactam residue. Under basic conditions, probe B+ shows only weak fluorescence from the cyanine acceptor while probe A displays nonfluorescence due to retention of the closed spirolactam form of the rhodamine moiety. Upon decrease in solution pH level, probe B+ exhibits a gradual fluorescence increase from rhodamine and cyanine constituents at 623 nm and 743 nm respectively, whereas probe A displays fluorescence increase at 623 nm on the rhodamine moiety as acidic conditions leads to the rupture of the probe spirolactam rings. Probes A and B+ have successfully been used to monitor intracellular pH alternations and possess pKa values of 5.15 and 7.80, respectively.

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Section: Resultsmentioning

confidence: 99%

A Near-Infrared Fluorescent Probe Based on a FRET Rhodamine Donor Linked to a Cyanine Acceptor for Sensitive Detection of Intracellular pH Alternations

Zhang

Xia

et al. 2018

Molecules

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“…Utilizing the metachromatic nature of toluidine blue, the stained tissue was fluoresced using a 488 nm laser [36] in order to image the mast cells which contained an altered Stokes shift in the metachromatically shifted dye. [37] This allowed imaging of the mast cells which were fluorescent under 488 nm illumination and the emission was captured at 647nm. [38] Images were obtained using an Olympus laser scanning confocal microscope and associated Fluoview software (Olympus America, Melville, NY) and analyzed using ImageJ.…”

Section: Toluidine Blue Stainingmentioning

confidence: 99%

Increased blood-brain barrier hyperpermeability coincides with mast cell activation early under cuprizone administration

et al. 2020

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The cuprizone induced animal model of demyelination is characterized by demyelination in many regions of the brain with high levels of demyelination in the corpus callosum as well as changes in neuronal function by 4-6 weeks of exposure. The model is used as a tool to study demyelination and subsequent degeneration as well as therapeutic interventions on these effects. Historically, the cuprizone model has been shown to contain no alterations to blood-brain barrier integrity, a key feature in many diseases that affect the central nervous system. Cuprizone is generally administered for 4-6 weeks to obtain maximal demyelination and degeneration. However, emerging evidence has shown that the effects of cuprizone on the brain may occur earlier than measurable gross demyelination. This study sought to investigate changes to blood-brain barrier permeability early in cuprizone administration. Results showed an increase in blood-brain barrier permeability and changes in tight junction protein expression as early as 3 days after beginning cuprizone treatment. These changes preceded glial morphological activation and demyelination known to occur during cuprizone administration. Increases in mast cell presence and activity were measured alongside the increased permeability implicating mast cells as a potential source for the blood-brain barrier disruption. These results provide further evidence of blood-brain barrier alterations in the cuprizone model and a target of therapeutic intervention in the prevention of cuprizoneinduced pathology. Understanding how mast cells become activated under cuprizone and if they contribute to blood-brain barrier alterations may give further insight into how and when the blood-brain barrier is affected in CNS diseases. In summary, cuprizone administration causes an increase in blood-brain barrier permeability and this permeability coincides with mast cell activation.

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“…HBA assisted ASPL has attracted growing interest in recent years due to potential application in various fields, such as photovoltaic energy conversion [50], photoluminescence bioimaging [51], thermal probes [52], optical refrigeration of solids [53][54][55][56], etc. It was shown that this type of up-conversion can take place both in inorganic materials, such as rare-earthdoped crystals [57][58][59], II-VI [60,61], InP [62], PbS [63] semiconductor quantum dots, InP and GaAs bulk semiconductors [64], inorganic perovskite nanoparticles [65][66][67], carbon nanotubes [68], and some organic dyes based on rhodamine [69], porphyrin [70], spirolactam moieties under acidic conditions [71], polymethine [72], fluorescein and oxazine [49].…”

Section: Thermal-assisted Up-conversionmentioning

confidence: 99%

Harvesting of the infrared energy: Direct collection, up-conversion, and storage

Dimitriev¹

2019

Semicond. phys. quantum electr. optoelectron

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Infrared (IR) energy constitutes almost a half of the solar radiation coming to the Earth surface and almost 100% of the outgoing terrestrial radiation. Surprisingly, but such a huge energy flux is mostly considered as thermal waste and is not used for the practical needs on the large scale. Here, two major methods of the IR energy collection have been briefly reviewed, the direct one and harvesting through up-conversion to the visible range, where this energy can be picked up by the conventional solar cells. Advantages and disadvantages of the above methods have been discussed. Potential of application of IR dyes for IR energy collection has been demonstrated. Storage of the IR energy as a delayed way of its consumption has been also discussed.

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New Near-Infrared Fluorescent Probes with Single-Photon Anti-Stokes-Shift Fluorescence for Sensitive Determination of pH Variances in Lysosomes with a Double-Checked Capability

Cited by 40 publications

References 45 publications

A Near-Infrared Fluorescent Probe Based on a FRET Rhodamine Donor Linked to a Cyanine Acceptor for Sensitive Detection of Intracellular pH Alternations

A Near-Infrared Fluorescent Probe Based on a FRET Rhodamine Donor Linked to a Cyanine Acceptor for Sensitive Detection of Intracellular pH Alternations

Increased blood-brain barrier hyperpermeability coincides with mast cell activation early under cuprizone administration

Harvesting of the infrared energy: Direct collection, up-conversion, and storage

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