New occupational allergen in citrus farmers: citrus red mite (Panonychus citri)

Kim, Yoon Keun; Son, Ji‐Won; Kim, Hee Yeon; Park, Hae‐Sim; Lee, Myung Hyun; Cho, Sang Heon; Min, Kyung Up; Kim, You Young

doi:10.1016/s1081-1206(10)62601-4

Cited by 32 publications

(28 citation statements)

References 23 publications

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“…juice and jam) in the European and American diet. However, studies on orange allergy are very limited [1,2,3,4,5,6,7], and most of them have dealt with non-orange allergens (enzymes used in fruit processing [4] or from citrus mites [5]), minor non-protein components of citrus peel oil [3] or non-IgE-mediated reactions [6]. Accordingly, no orange fruit allergen has been isolated and characterized to date.…”

Section: Introductionmentioning

confidence: 99%

Lipid Transfer Proteins and Allergy to Oranges

Ahrazem¹,

Ibáñez

López‐Torrejón³

et al. 2005

Int Arch Allergy Immunol

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Background: Lipid transfer proteins (LTPs) are relevant fruit allergens, recently proposed as model plant food allergens. No citrus fruit allergen has been characterized to date. We sought to identify and isolate citrus fruit LTPs and to explore their relevance in orange allergy. Methods: Twenty-seven patients, showing mainly oral allergy syndrome after orange ingestion, as well as positive prick responses and serum-specific IgE levels to orange, were selected. Natural orange and lemon LTPs, as well as a recombinant orange LTP isoform expressed in Pichia pastoris, were isolated by chromatographic methods and characterized by N-terminal amino acid sequencing and matrix-assisted laser desorption/ionizaion mass spectrometry, and DNA sequencing of the corresponding cDNA in the case of the recombinant allergen. Specific IgE determination, immunodetection, ELISA-inhibition assays and in vivo skin prick tests (SPTs) were performed with all three purified allergens and with the major peach LTP allergen, Pru p 3. Results: The natural allergens purified from orange (nCit s 3) and lemon (nCit l 3) showed very similar N-terminal amino acid sequences (18 out of 20 identical residues), typical of LTPs, and molecular masses of 9,610 and 9,618 Da, respectively. The recombinant orange isoform (rCit s 3) expressed in P. pastoris (16 out of 20 residues identical to its natural counterpart in the N-terminal region) presented 92 amino acid residues and 9,463 Da, and 67% sequence identity with rPru p 3. Of the 27 sera analyzed, specific IgE to the purified allergens was found in 54% for nCit l 3, 48% for nCit s 3, 46% for rCit s 3 and 37% for rPru p 3. Positive SPT responses were obtained in 7 out of 26 patients tested for nCit s 3, 3 out of 8 for nCit l 3 and 10 out of 26 for nPru p 3. ELISA-inhibition assays showed an equivalent IgE-binding pattern for the natural and recombinant orange LTPs, and IgE cross-reactivity among the purified orange, lemon and peach LTP allergens. Conclusions: Members of the LTP allergen family are involved in allergy to oranges, displaying positive in vitro and in vivo reactions in 30–50% of the patients studied. Both orange and lemon allergens show cross-reactivity with the major peach allergen Pru p 3.

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Section: Introductionmentioning

confidence: 99%

Lipid Transfer Proteins and Allergy to Oranges

Ahrazem¹,

Ibáñez

López‐Torrejón³

et al. 2005

Int Arch Allergy Immunol

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“…There have been several reports of occupational allergy to Tetranychidae [5][6][7], and we recently reported 16 cases of CRM-induced asthma in citrus farmers who visited our clinic [8]. In this study, a cross-sectional survey was performed to evaluate type I hypersensitivity to CRM, its prevalence, and relationship to respiratory dysfunction in citrus farmers on Cheju Island.…”

Section: Introductionmentioning

confidence: 99%

Citrus red mite (Panonychus citri) is the most common sensitizing allergen of asthma and rhinitis in citrus farmers

Kim

Son

Kim

et al. 1999

Clin Experimental Allergy

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SummaryObjective To evaluate type I hypersensitivity to citrus red mite (Panonychus citri), its prevalence, and relationship to respiratory dysfunction, a cross-sectional survey was performed among citrus farmers on Cheju Island, Korea. Materials and methods Questionnaires, and skin prick test responses to 11 common inhalant allergens and citrus red mite were performed in 181 citrus farmers, and serumspecific IgE antibodies to citrus red mite were measured by ELISA in sera of 123 subjects. To determine airway hyperresponsiveness, methacholine bronchial provocation tests were performed in 55 subjects who complained of recurrent lower respiratory symptoms. Results The prevalence of asthma-based on presence of asthmatic symptoms on the questionnaire and airway hyperresponsiveness to methacholine, and allergic rhinitis based on presence of nasal symptoms on the questionnaire and positive skin-test response were 12.1% and 19.3%, respectively. The positive rate of skin responses to one or more of 11 common inhalant allergens excluding citrus red mite was 17.1%, and if citrus red mite was included, 25.9% of farmers had positive responses. On skin prick tests, citrus red mite (16.5%) was the most common sensitizing allergen, followed by cockroach (11.0%), Dermatophagoides pteronyssinus (9.9%), and D. farinae (9.3%). Among farmers with asthma and allergic rhinitis, the positive skin responses to citrus red mite were noted in 54.5 and 68.5%, respectively. Serum-specific IgE antibodies to citrus red mite were detected in 45 farmers (36.5%) of the 123 tested, and there was significant correlation between specific IgE level and weal (A/H ratio) to citrus red mite (r ¼ 0.57, P < 0.001). The prevalence of asthma was higher in subjects with positive skin responses or high serum-specific IgE antibodies to citrus red mite than in those without skin response or serum specific IgE (P < 0.05, respectively). Conclusion Citrus red mite is the most important allergen in citrus farmers with asthma and rhinitis in which causative allergen has not been identified. It should be included in the skin test battery for screening the causative allergen in farmers exposed to citrus red mite.

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“…A positive control of histamine (1 mg/mL, Allergopharma Co., Germany) along with a negative control of diluent (Allergopharma Co., Germany) was included in all tests. CRM and Japanese cedar pollen extracts were prepared as previously described [9]. Brie¯y, CRM and Japanese cedar pollen were mixed with modi®ed Coca's solution in 1 : 20 w/v ratio.…”

Section: Methodsmentioning

confidence: 99%

Bronchial responsiveness to methacholine is increased in citrus red mite (Panonychus citri)‐sensitive children without asthmatic symptoms

Min

Park

Lee

et al. 2000

Clin Experimental Allergy

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SummaryBackground A recent investigation has suggested that citrus red mite (Panonychus citri, CRM) is a common sensitizing allergen among children living around citrus farms. Objective A cross-sectional survey was performed to evaluate the bronchial responsiveness to methacholine in CRM-sensitive children without asthmatic symptoms. Subjects and methods A total of 375 children living around citrus farms were enrolled in this study. There were 201 boys and 174 girls with a mean age of 12.5 (range 7±16) years. Each subject was evaluated by a questionnaire, a skin prick test with 13 common inhalant allergens including CRM, and a methacholine bronchial provocation test (MBPT). Results Positive rate of MBPT (PC 20 -methacholine < 25 mg/mL) was higher in atopic rhinitic and atopic control children than in nonatopic rhinitic and nonatopic control subjects (41.3% and 33.3% vs 17.2% and 15.4%, respectively, both P < 0.05). Slope of dose± response curve (DRS, %/mg/mL) was higher in atopic rhinitic children than in nonatopic rhinitic, atopic control and nonatopic control subjects (14.3 6 0.87 vs 9.4 6 0.72, 10.0 6 1.37 and 9.2 6 1.51, P < 0.05, respectively). Both the positive rate of MBPT and the DRS were increased in children with positive skin response to CRM than in those without sensitization (48.2% vs 22.9%, P 0.0001; 15.6 6 1.26 vs 10.2 6 0.65, P 0.0001). Conclusion Bronchial responsiveness is increased in citrus red mite-sensitive children even if they have no asthmatic symptoms.

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New occupational allergen in citrus farmers: citrus red mite (Panonychus citri)

Cited by 32 publications

References 23 publications

Lipid Transfer Proteins and Allergy to Oranges

Lipid Transfer Proteins and Allergy to Oranges

Citrus red mite (Panonychus citri) is the most common sensitizing allergen of asthma and rhinitis in citrus farmers

Bronchial responsiveness to methacholine is increased in citrus red mite (Panonychus citri)‐sensitive children without asthmatic symptoms

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