New Opportunities of Electrochemistry for Monitoring, Modulating, and Mimicking the Brain Signals

Wu, Fei; Yu, Ping; Mao, Lanqun

doi:10.1021/jacsau.3c00220

Cited by 5 publications

(3 citation statements)

References 87 publications

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“…Till now, great efforts have been devoted to address the selectivity issue in the development of in vivo GRP sensors because of the ubiquitous interference from coexisting neurochemicals with similar redox potentials. We have designed a series of brain–electrode sensing interfaces directed by the formal potential ( E 0′ ) ordering strategy, aiming at tuning the heterogeneous electron transfer kinetics and thus achieving the selectivity for the reduction or oxidation of neurochemicals . To this end, we have achieved selective in vivo sensing of ascorbate, hydrogen sulfide (H 2 S), dioxygen, serotonin, and so forth, forming a basis for GRP sensing in vivo. ,− …”

Section: Introductionmentioning

confidence: 99%

Enzymatic Galvanic Redox Potentiometry for In Vivo Biosensing

Lu,

Zhuang,

Wei

et al. 2024

Anal. Chem.

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Redox potentiometry has emerged as a new platform for in vivo sensing, with improved neuronal compatibility and strong tolerance against sensitivity variation caused by protein fouling. Although enzymes show great possibilities in the fabrication of selective redox potentiometry, the fabrication of an enzyme electrode to output open-circuit voltage (E OC ) with fast response remains challenging. Herein, we report a concept of novel enzymatic galvanic redox potentiometry (GRP) with improved time response coupling the merits of the high selectivity of enzyme electrodes with the excellent biocompatibility and reliability of GRP sensors. With a glucose biosensor as an illustration, we use flavin adenine dinucleotide-dependent glucose dehydrogenase as the recognition element and carbon black as the potential relay station to improve the response time. We find that the enzymatic GRP biosensor rapidly responds to glucose with a good linear relationship between E OC and the logarithm of glucose concentration within a range from 100 μM to 2.65 mM. The GRP biosensor shows high selectivity over O 2 and coexisting neurochemicals, good reversibility, and sensitivity and can in vivo monitor glucose dynamics in rat brain. We believe that this study will pave a new platform for the in vivo potentiometric biosensing of chemical events with high reliability.

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Section: Introductionmentioning

confidence: 99%

Enzymatic Galvanic Redox Potentiometry for In Vivo Biosensing

Lu,

Zhuang,

Wei

et al. 2024

Anal. Chem.

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“…Neurological activity is essentially a series of changes in chemical and electrochemical signals caused by the transmission and regulation of neurochemicals, and the interactions of these neurochemicals (neurotransmitters, neuromodulators, various ions, gases, proteins, energy metabolizers, etc.) form a complex neuromodulatory network. − Deciphering neurochemical signals is crucial for understanding chemical changes in the brain during physiological and pathological processes, which requires higher spatiotemporal resolution, selectivity, sensitivity, and stability of analytical techniques. − In recent years, electrochemical sensing platforms based on a carbon fiber microelectrode (CFE) that allow real-time and in situ monitoring of neurotransmitters have become one of the most dynamic approaches for in vivo analysis. − However, designing recognition molecules and customizing functionalized surfaces to develop in vivo electrochemical biosensors with high selectivity and stability are major challenges today.…”

Section: Introductionmentioning

confidence: 99%

Universal Covalent Grafting Strategy of an Aptamer on a Carbon Fiber Microelectrode for Selective Determination of Dopamine In Vivo

Chen,

Xia,

Ding

et al. 2024

Anal. Chem.

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The chemical information on brain science provided by electrochemical sensors is critical for understanding brain chemistry during physiological and pathological processes. A major challenge is the selectivity of electrochemical sensors in vivo. This work developed a universal covalent grafting strategy of an aptamer on a carbon fiber microelectrode (CFE) for selective determination of dopamine in vivo. The universal strategy was proposed by oxidizing poly(tannic acid) (pTA) to form an oxidized state (pTA ox ) and then coupling a nucleophilic sulfhydryl molecule of the dopamine-binding mercapto-aptamer with the oquinone moiety of pTA ox based on click chemistry for the interfacial functionalization of the CFE surface. It was found that the universal strategy proposed could efficiently graft the aptamer on a glassy carbon electrode, which was verified by using electroactive 6-(ferrocenyl) hexanethiol as a redox reporter. The amperometric method using a fabricated aptasensor for the determination of dopamine was developed. The linear range of the aptasensor for the determination of dopamine was 0.2−20 μM with a sensitivity of 0.09 nA/μM and a limit of detection of 88 nM (S/N = 3). The developed method has high selectivity originating from the specific recognition of the aptamer in concert with the cation-selective action of pTA and could be easily applicable to probe dopamine dynamics in the brain. Furthermore, complex vesicle fusion modes were first observed at the animal level. This work demonstrated that the covalently grafted immobilization strategy proposed is promising and could be extended to the in vivo analysis of other neurochemicals.

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“…Recent studies have fabricated different kinds of sensors for dopamine detection, using for example specific surface functionalizations, DNA aptamer-based biosensors, field–effect transistors (FETs) or electrolyte-gated transistors (EGTs), surface-enhanced Raman spectroscopy, or a combination of all of them [ 21 , 22 , 23 ]. Carbon electrodes can be functionalized with different modifications like nanomaterials from metals or metal oxides, semiconductors, polymers, hydrophilic materials, thiols, enzymes, etc.…”

Section: Introductionmentioning

confidence: 99%

Development of a Smart Wireless Multisensor Platform for an Optogenetic Brain Implant

Cunha,

Schuelke,

Mesri

et al. 2024

Sensors

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Implantable cell replacement therapies promise to completely restore the function of neural structures, possibly changing how we currently perceive the onset of neurodegenerative diseases. One of the major clinical hurdles for the routine implementation of stem cell therapies is poor cell retention and survival, demanding the need to better understand these mechanisms while providing precise and scalable approaches to monitor these cell-based therapies in both pre-clinical and clinical scenarios. This poses significant multidisciplinary challenges regarding planning, defining the methodology and requirements, prototyping and different stages of testing. Aiming toward an optogenetic neural stem cell implant controlled by a smart wireless electronic frontend, we show how an iterative development methodology coupled with a modular design philosophy can mitigate some of these challenges. In this study, we present a miniaturized, wireless-controlled, modular multisensor platform with fully interfaced electronics featuring three different modules: an impedance analyzer, a potentiostat and an optical stimulator. We show the application of the platform for electrical impedance spectroscopy-based cell monitoring, optical stimulation to induce dopamine release from optogenetically modified neurons and a potentiostat for cyclic voltammetry and amperometric detection of dopamine release. The multisensor platform is designed to be used as an opto-electric headstage for future in vivo animal experiments.

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New Opportunities of Electrochemistry for Monitoring, Modulating, and Mimicking the Brain Signals

Cited by 5 publications

References 87 publications

Enzymatic Galvanic Redox Potentiometry for In Vivo Biosensing

Enzymatic Galvanic Redox Potentiometry for In Vivo Biosensing

Universal Covalent Grafting Strategy of an Aptamer on a Carbon Fiber Microelectrode for Selective Determination of Dopamine In Vivo

Development of a Smart Wireless Multisensor Platform for an Optogenetic Brain Implant

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