New paradigm for imaging systems

Cathey, W. Thomas; Dowski, Edward R.

doi:10.1364/ao.41.006080

Cited by 320 publications

(153 citation statements)

References 12 publications

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“…32 The EDF version of the ImageStream system incorporates a specialized optical element in the standard optical system that causes light from widely different focal positions in the object to be imaged on the detector plane simultaneously in a process referred to as Wavefront Coding™ by its developer, CDM Optics, Inc. (Boulder, CO). 33 The modified imagery is post-processed to recover image sharpness while preserving the increased depth of focus that comes from the modification of the wavefront during data acquisition. Images acquired using the EDF version of the system have an effective depth of field of approximately 15 microns, resulting in a high resolution image of the cell with all features simultaneously in focus.…”

Section: High Throughput Extended Depth Of Field Imaging Of Cells Submentioning

confidence: 99%

Cellular Image Analysis and Imaging by Flow Cytometry

Basiji

Ortyn

Liang

et al. 2007

Clinics in Laboratory Medicine

373

288

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SynopsisImaging flow cytometry combines the statistical power and fluorescence sensitivity of standard flow cytometry with the spatial resolution and quantitative morphology of digital microscopy. The technique is a good fit for clinical applications by providing a convenient means for imaging and analyzing cells directly in bodily fluids. Examples are provided of the discrimination of cancerous from normal mammary epithelial cells and the high throughput quantitation of FISH probes in human peripheral blood mononuclear cells. The FISH application will be further enhanced by the integration of extended depth of field imaging technology with the current optical system. Keywordsimaging; flow; cytometry; fluorescence; brightfield; darkfield; multispectral Quantifying Cellular Structure in Health and DiseaseThe eukaryotic cell is a highly structured, three-dimensional object containing a wide range of molecular species. The size, shape, and structure of the cell, as well as the abundance, location, and co-location of any of these constituent biomolecules may be of significance in any given clinical situation or research application. For instance, in hematopoiesis, as cells differentiate and mature, different subsets of molecules are expressed that reflect a specialized functional capacity for that unique cell type (e.g., granulocytes vs. lymphocytes). In general the characterization of this array of constituent molecules by imaging or flow cytometry provides insight into the physiological function of any particular cell or alternatively, pathological changes that may have occurred or accrued. In clinical practice and in research settings, cellular evaluation by imaging technologies and flow cytometry provides significant information reflecting the particular cellular phenotype, both normal and pathological. Microscopy provides a wealth of information, but data acquisition rates are slow and analysis is generally subjective. In flow cytometry, data acquisition is rapid and better suited for the evaluation of pathologies present in low frequency, but the data are only intensity-based, thus lacking the morphology that truly lends credence to the analysis.In addition, the assessment and evaluation of cell samples by imaging and flow cytometric techniques is complicated by a number of factors. For instance, changes in a cell type or aCorresponding author for proofs and reprints:

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Section: High Throughput Extended Depth Of Field Imaging Of Cells Submentioning

confidence: 99%

Cellular Image Analysis and Imaging by Flow Cytometry

Basiji

Ortyn

Liang

et al. 2007

Clinics in Laboratory Medicine

373

288

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“…To extend DOF, several phase distributions have been proposed; [1], [2], [16]. The cubic plate is the most common and widely used:…”

Section: B Wavefront Codingmentioning

confidence: 99%

“…Wavefront coding (WFC) is a state-of-the-art technique that enables DOF improvement without jeopardizing other design parameters such as the luminosity of the instrument. WFC was first proposed by Dowsky and Cathey [1], [2] and nowadays is widely used in the design of optical setups [3]- [5]. The technique is based on the modification of the wavefront (or wavefront coding, using Dowsky and Cathey words) by means of a suitable phase mask (PM) placed at the aperture stop of the system.…”

Section: Introductionmentioning

confidence: 99%

Design of Optical Systems With Extended Depth of Field: An Educational Approach to Wavefront Coding Techniques

2012

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A practical activity designed to introduce wavefront coding techniques as a method to extend the depth of field in optical systems is presented. The activity is suitable for advanced undergraduate students since it combines different topics in optical engineering such as optical system design, aberration theory, Fourier optics, and digital image processing. The paper provides theoretical background and technical information for performing the experiment. The proposed activity requires students able to develop a wide range of skills, since they are expected to deal with optical components, including spatial light modulators, and develop scripts to perform some calculations.

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“…Dowski y W.T. Cathey [3,4] propusieron una técnica novedosa denominada Wavefront Coding (WFC) con el fin de obtener un sistema óptico que proporcionara mayor calidad de imagen en presencia de aberraciones como desenfoque, astigmatismo, o coma. La técnica combina una etapa de codificación del frente de onda mediante una lámina de fase cúbica (LFC) que proporciona una respuesta de impulso del sistema invariante a la presencia de aberraciones, con una etapa de post-procesado que se encarga de decodificar la imagen, actualmente esta técnica se emplea en sistemas de reconocimiento de iris y de huella dactilar, y en cámaras de teléfonos móvil sin sistema de enfoque [5,6].…”

Section: Introductionunclassified

Use of a cubic phase plate as solution for moderate astigmatism: preliminary study

Almaguer¹,

Arines²

2017

Opt. Pura Apl.

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ABSTRACT:This work analyzes the use of a single cubic phasemask as a solution to moderate astigmatism of different orientations and magnitudes. The experimental results with artificial eye show that a cubic phasemask of the type 7.07 µm×(Z3 3 -Z3 -3 ) @ 5mm pupillary diameter, can be a solution to the astigmatism up to 3D with different orientations. The logMAR visual acuity obtained for all orientations is close to 0.05 for pupil sizes of 3 mm, 5 mm, 7 mm. These results are a good starting point for the development of contact lenses or intraocular lenses that do not require stabilization systems increasing tolerance to errors in the correction of the astigmatism.Key words:Astigmatism, neural transfer function, cubic phasemask, Intraocular Lenses, Contact Lenses, visual acuity. RESUMEN:En este trabajo se explora la posibilidad de utilizar una única lámina de fase cúbica como solución a astigmatismos moderados de distintas magnitudes y orientaciones. Los resultados experimentales obtenidos con ojo artificial muestran que una fase cúbica del tipo 7.07 µm×(Z3 3 -Z3 -3 ) @5mm diámetro pupilar, puede ser una solución al astigmatismo de hasta 3D con diferentes orientaciones. La agudeza visual logMAR obtenida para todas las orientaciones es superior a 0.05 logMar para tamaños de pupilas de 3 mm, 5 mm, 7 mm. Estos resultados son un buen punto de partida para el desarrollo de lentes de contacto o lentes intraoculares con menos dependencia a la estabilización y que incrementen la tolerancia a errores en la determinación del eje y potencia del astigmatismo refractivo.Palabras clave: Astigmatismo, función de transferencia neuronal, lámina de fase cúbica, lentes intraoculares, lentes de contacto, agudeza visual.

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New paradigm for imaging systems

Cited by 320 publications

References 12 publications

Cellular Image Analysis and Imaging by Flow Cytometry

Cellular Image Analysis and Imaging by Flow Cytometry

Design of Optical Systems With Extended Depth of Field: An Educational Approach to Wavefront Coding Techniques

Use of a cubic phase plate as solution for moderate astigmatism: preliminary study

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