New peak broadening parameter for the characterization of separation capability in capillary electrophoresis

Sursyakova, Viktoria V.; Rubaylo, Anatoly I.

doi:10.1002/jssc.201401109

Cited by 10 publications

(12 citation statements)

References 50 publications

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“…We consider this latter efficiency value to be adequate for CE-MS analysis of samples possessing strong negative charges. Our findings are in full agreement with the work of Sursyakova and Rubaylo [15] and others [25,26]. The reported flow profiles well visualize and explain the underlying flow dynamics phenomena, i.e., the superposed flat EOF and parabola-like profiles and their effect on separation efficiency.…”

Section: Concluding Remark Ssupporting

confidence: 92%

Effect of the flow profile on separation efficiency in pressure‐assisted reversed‐polarity capillary zone electrophoresis of anions: Simulation and experimental evaluation

Járvás

Szigeti

Guttman

2018

J of Separation Science

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Capillary electrophoresis connected to electrospray ionization mass spectrometry is a promising combination to analyze complex biological samples. The use of sheathless electrospray ionization interfaces, such as a porous nanoelectrospray capillary emitter, requires the application of forward flow (either by pressure or electroosmosis) to maintain the electrospray process. The analysis of solute molecules with strong negative charges (e.g., aminopyrenetrisulfonate labeled glycans) necessitates a reversed-polarity capillary electrophoresis separation mode, in which case the electroosmotic flow is counter current, thus pressure assistance is necessary. In this study, we compared the effect of forced convection with and without counter electroosmotic flow on the resulting separation efficiency in capillary electrophoresis based on flow profile simulations by computational fluid dynamics technique and by actual experiments. The efficiencies of the detected peaks were calculated from the resulting electropherograms and found approximately 950 000 plates/m for electrophoresis with counter electroosmotic flow, 20 000 plates/m with pressure only (such as would be in open tubular liquid chromatography), and 480 000 plates/m for electrophoresis with simultaneous counter electroosmotic flow and forward pressure assistance, which validates the simulation data.

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Section: Concluding Remark Ssupporting

confidence: 92%

Effect of the flow profile on separation efficiency in pressure‐assisted reversed‐polarity capillary zone electrophoresis of anions: Simulation and experimental evaluation

Járvás

Szigeti

Guttman

2018

J of Separation Science

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“…where k = 0.50 for |z| = 1 and k = 0.77 for |z| >1. The peak width in terms of electrophoretic mobility [35] and theoretical values of electroosmotic mobility [40] were calculated using Eqs. S5 and S6, respectively.…”

Section: Calculationsmentioning

confidence: 99%

“…The studies with the theoretical consideration of the peak width are few and a number of assumptions used are valid at infinite dilution [29,33,34]. Recently, a new peak broadening parameter has been suggested for characterization of the separation capability in capillary electrophoresis [35]. This is the peak width in terms of electrophoretic mobility at 5 % of the maximum peak height w 0.05 (µ).…”

Section: Introductionmentioning

confidence: 99%

Strategy for non-target ionic analysis by capillary electrophoresis with ultraviolet detection

2016

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A strategy for non-target analysis of samples with unknown composition by capillary electrophoresis (CE) with ultraviolet (UV) detection is suggested. The strategy is based on the preliminary identification of analytes and further optimization of the conditions for their separation using the developed computational tool set ElphoSeparation. It is shown that, in order to record electrophoretic peaks with the mobilities from the maximum to minimum possible values, the positive and negative voltage polarity and hydrodynamic pressure should be used. To choose the optimal separation conditions, dynamic maps of electrophoretic separation (DMES) are suggested. DMES is a bar chart with theoretical resolutions of adjacent peaks presented in ascending order of the migration time. The resolution is calculated as the division of the difference of the effective electrophoretic mobilities of adjacent analytes by their average peak width in terms of electrophoretic mobility. The suggested strategy is tested by the example of the analysis of herbal medicine (Holosas) on the basis of rose hips. The approach should be used to analyze samples with not very complex composition, such as environmental water and precipitation samples, process liquors, some vegetable extracts, biological fluids, food, and other samples for the determination of widespread compounds capable of forming ionic species. For samples with complex composition, the approach used together with other techniques may produce advantageous information due to specificity of the method, particularly it can be useful for determination of compounds suffering from low volatility or thermal stability, and for analysis of samples with difficult matrices. Graphical Abstract The scheme of performing the non-target ionic analysis by capillary electrophoresis with ultraviolet detection.

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“…The detection wavelengths were 254 and 191 nm. The hydrodynamic pressure of 50 mbar was used to inject samples for 2 s and to reduce migration time during the separation [41][42][43][44].…”

Section: Instrumentationmentioning

confidence: 99%

Composition and stability constants of copper(II) complexes with succinic acid determined by capillary electrophoresis

Sursyakova

Burmakina

Rubaylo

2016

Journal of Coordination Chemistry

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Fax: 73912494108 2 Composition and stability constants of copper(II) complexes with succinic acid ions determined by capillary electrophoresisThe advantage of usage of capillary electrophoresis technique was demonstrated for studying a complicated system owing to the dependence of direction and velocity of the electrophoretic movement on the charge of complex species. The stability constants of copper(II) complexes with ions of succinic acid were determined by capillary electrophoresis, including the 1:2 metal to ligand complexes which are rarely mentioned and taken into account in studies during recent years. The measurements were carried out at 25 ºC and ionic strength of 0.1, obtained by mixing the solutions of succinic acid and lithium hydroxide up to рН 4.2 -6.2. It was shown that while pH was more than 4.5 the zone of copper(II) complexes with succinate ions moves as an anion. It is impossible to treat this fact using only the complexes with a metal-ligand ratio of 1:1 (CuL 0 , CuHL + ).The following values of stability constants were obtained: log β(CuL) = 2.89 ± 0.02, log β(CuHL + ) = 5.4 ± 0.5, log β(CuL 2 2-) = 3.88 ± 0.05, log β(CuHL 2 -) = 7.2 ± 0.3.

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New peak broadening parameter for the characterization of separation capability in capillary electrophoresis

Cited by 10 publications

References 50 publications

Effect of the flow profile on separation efficiency in pressure‐assisted reversed‐polarity capillary zone electrophoresis of anions: Simulation and experimental evaluation

Effect of the flow profile on separation efficiency in pressure‐assisted reversed‐polarity capillary zone electrophoresis of anions: Simulation and experimental evaluation

Strategy for non-target ionic analysis by capillary electrophoresis with ultraviolet detection

Composition and stability constants of copper(II) complexes with succinic acid determined by capillary electrophoresis

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