AIM: To investigate the clinical effect of low power He Ne laser in the treatment of children's refractive amblyopia, and to analyze the necessary reference for the comprehensive treatment of children's refractive amblyopia. METHODS: 112 cases diagnosed (176 eyes) with refractive amblyopic children according to the visiting sequence were randomly divided into treatment group and control group, control group of children with conventional synthetic amblyopia therapeutic apparatus in the treatment, and treatment group attached children helium neon laser project suffering from ocular irradiation treatment, observation of visual function after record 3-6 months, fundus and electrophysiological changes. RESULTS: in the treatment group, the cure rate of mild, moderate and severe amblyopia after 3 months were 82.2%, 80.6%, 36.4%, the cure rate was 95.6%, 88.9%, 63.6% after 6 months. In the control group, the cure rate was 47.7%, 20% and 0% after 3 months, the cure rate was 54.5%, 30%, 12.5% after 6 months, respectively. Statistical analysis showed that the treatment group was better than the control group in the visual function of children with amblyopia (P<0.05), and shortened the recovery time of the visual function of the children with amblyopia, and there was no abnormal changes in the fundus of the eyes. Conclusion: low power He Ne laser used in the comprehensive treatment of children with refractive amblyopia, improve the cure rate of amblyopia in children, and shorten the treatment of amblyopia treatment recovery, safe and effective.