New perspectives for microbial glycolipid fractionation and purification processes

Hubert, Jane; Plé, Karen; Hamzaoui, Mahmoud; Nuissier, Gladys; Hadef, Imane; Reynaud, Romain; Guilleret, Arnaud; Renault, Jean‐Hugues

doi:10.1016/j.crci.2011.11.002

Cited by 21 publications

(14 citation statements)

References 87 publications

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“…TL are present as trehalose dimicolate in bacteria such as mycobacteria, Corynebacterium , Nocardia and Rhodococcus [ 108 ]. Most TL are found in these prokaryotes cell walls [ 109 , 110 ], which can make it difficult to extract. In addition, most of these strains can exhibit slow growth and may be pathogenic.…”

Section: Structural Classes Properties and Applicationsmentioning

confidence: 99%

Fungal biosurfactants, from nature to biotechnological product: bioprospection, production and potential applications

Silva

Banat

Giachini

et al. 2021

Bioprocess Biosyst Eng

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Biosurfactants are in demand by the global market as natural commodities that can be added to commercial products or use in environmental applications. These biomolecules reduce the surface/interfacial tension between fluid phases and exhibit superior stability to chemical surfactants under different physico-chemical conditions. Biotechnological production of biosurfactants is still emerging. Fungi are promising producers of these molecules with unique chemical structures, such as sophorolipids, mannosylerythritol lipids, cellobiose lipids, xylolipids, polyol lipids and hydrophobins. In this review, we aimed to contextualize concepts related to fungal biosurfactant production and its application in industry and the environment. Concepts related to the thermodynamic and physico-chemical properties of biosurfactants are presented, which allows detailed analysis of their structural and application. Promising niches for isolating biosurfactant-producing fungi are presented, as well as screening methodologies are discussed. Finally, strategies related to process parameters and variables, simultaneous production, process optimization through statistical and genetic tools, downstream processing and some aspects of commercial products formulations are presented.

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Section: Structural Classes Properties and Applicationsmentioning

confidence: 99%

Fungal biosurfactants, from nature to biotechnological product: bioprospection, production and potential applications

Silva

Banat

Giachini

et al. 2021

Bioprocess Biosyst Eng

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“…In other words, the type of fatty acids does not affect the classification of MEL homologues (Hubert et al 2012).…”

Section: Determination Of Mel Concentrationmentioning

confidence: 99%

“…To the best of our knowledge, the application of UF for purification of MEL has not been investigated yet. Purification of MEL is typically carried out by As highlighted by Hubert et al (2012), much research has focused on reducing production costs of glycolipids that are synthesized by microorganisms. In the present work a novel bioprocess was developed which could result in a more cost effective process.…”

Section: Introductionmentioning

confidence: 99%

A novel approach for the production and purification of mannosylerythritol lipids (MEL) by Pseudozyma tsukubaensis using cassava wastewater as substrate

Andrade

Rocco

et al. 2017

Separation and Purification Technology

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P. tsukubaensis is a yeast-like microorganism that synthesized the biosurfactant mannosylerythritol lipids-B (MEL-B). Production cost can be one of the drawbacks of biosurfactants production. Therefore the development of efficient and cost effective purification strategies and the use of by-products in the culture medium could serve as important strategies to reduce overall process cost. The aim of this work was to evaluate the production of MEL using cassava wastewater, a hydrophilic medium composed of a low-cost substrate which is a by-product of cassava processing, followed by foam fractionation and ultrafiltration of MEL. Cassava wastewater proved to be a feasible culture medium for P. tsukubaensis and MEL-B production as the yield (1.26 g L) was similar to that reported by others using water-soluble carbon sources (up to 2 g/L). Interestingly ultrafiltration with 100 KDa MWCO memabranes (using 20 mL centrifugal devices) led to the purification of MEL-B in one step since ≈ 80% of MEL was recovered, while more than 95% of proteins were found in the permeate. The scale up of the ultrafiltration (up to 500 mL) using a cross flow filtration unit led to very similar results. Overall the ultrafiltration led to a threefold increase in MEL purity in terms of protein (at both scales). The chemical characterisation by NMR confirmed the production of MEL-B homologue and also the production of a second stereoisomer ≈ 9%, while the CG-MS and MALDI-TOFMS analysis confirmed the main fatty acids within the structure of MEL-B (C8:0 and 12:0 and C8:0 and C14:1). Therefore, the process developed here was found to be a good alterntative to the conventional production of MEL which uses synthetic culture medium, solvent extraction (ethyl acetate) and column chromatography (silica) for its purification.

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“…The industrial production of glycolipids started with sophorolipids in the last decade by several companies [ 7 , 8 ]. Besides sophorolipids, rhamnolipids (RLs) are the most studied glycolipids with industrial potential [ 1 , 9 , 10 , 11 , 12 ], as they, e.g., can be produced at titers above 35 g RL /L [ 13 , 14 ]. RLs consist of one or two rhamnose molecules, linked through a β-glycosidic bond to one or two 3-hydroxy fatty acid moieties [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Uncoupling Foam Fractionation and Foam Adsorption for Enhanced Biosurfactant Synthesis and Recovery

et al. 2020

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The production of biosurfactants is often hampered by excessive foaming in the bioreactor, impacting system scale-up and downstream processing. Foam fractionation was proposed to tackle this challenge by combining in situ product removal with a pre-purification step. In previous studies, foam fractionation was coupled to bioreactor operation, hence it was operated at suboptimal parameters. Here, we use an external fractionation column to decouple biosurfactant production from foam fractionation, enabling continuous surfactant separation, which is especially suited for system scale-up. As a subsequent product recovery step, continuous foam adsorption was integrated into the process. The configuration is evaluated for rhamnolipid (RL) or 3-(3-hydroxyalkanoyloxy)alkanoic acid (HAA, i.e., RL precursor) production by recombinant non-pathogenic Pseudomonas putida KT2440. Surfactant concentrations of 7.5 gRL/L and 2.0 gHAA/L were obtained in the fractionated foam. 4.7 g RLs and 2.8 g HAAs could be separated in the 2-stage recovery process within 36 h from a 2 L culture volume. With a culture volume scale-up to 9 L, 16 g RLs were adsorbed, and the space-time yield (STY) increased by 31% to 0.21 gRL/L·h. We demonstrate a well-performing process design for biosurfactant production and recovery as a contribution to a vital bioeconomy.

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New perspectives for microbial glycolipid fractionation and purification processes

Cited by 21 publications

References 87 publications

Fungal biosurfactants, from nature to biotechnological product: bioprospection, production and potential applications

Fungal biosurfactants, from nature to biotechnological product: bioprospection, production and potential applications

A novel approach for the production and purification of mannosylerythritol lipids (MEL) by Pseudozyma tsukubaensis using cassava wastewater as substrate

Uncoupling Foam Fractionation and Foam Adsorption for Enhanced Biosurfactant Synthesis and Recovery

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