2019
DOI: 10.2306/scienceasia1513-1874.2019.45.116
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New perspectives for surveying and improving Thai dried seafood qualities using antimicrobials produced by Bacillus velezensis BUU004 against foodborne pathogens

Abstract: Dried seafood products are one of the most popular seafood items in Thailand. This study was divided into two experiments. In first experiment, a survey in intrinsic and bacteriological characteristics of twenty dried seafood products distributed in Chon Buri province, Thailand was performed. Values of water activity, pH and NaCl content were 0.35 ± 0.01-0.72 ± 0.00, 5.47 ± 0.03-9.24 ± 0.01, and 0.66 ± 0.06-2.26 ± 0.03%, respectively. Based on the Thai regulatory standard, half of dried seafood products contai… Show more

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Cited by 7 publications
(12 citation statements)
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“…In our recent study, a semi-purified preparation (SPP) containing bacteriocin from Bacillus velezensis BUU004 exhibited broad-spectrum antibacterial activity against food-borne Gram-positive and Gram-negative bacteria (Butkhot et al, 2019a). However, unsatisfactory activity against E. coli and Salmonella Typhimurium was observed when the SPP alone was added to dried seasoned squid.…”
Section: Lemongrass (Cymbopogon Citratus (Dc)mentioning
confidence: 99%
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“…In our recent study, a semi-purified preparation (SPP) containing bacteriocin from Bacillus velezensis BUU004 exhibited broad-spectrum antibacterial activity against food-borne Gram-positive and Gram-negative bacteria (Butkhot et al, 2019a). However, unsatisfactory activity against E. coli and Salmonella Typhimurium was observed when the SPP alone was added to dried seasoned squid.…”
Section: Lemongrass (Cymbopogon Citratus (Dc)mentioning
confidence: 99%
“…A loopful of B. velezensis BUU004 strain was inoculated into TSB, and cultured at 30°C with shaking at 200 rpm for 18 h. Then, the supernatant containing bacteriocin was collected by centrifugation (Centrifuge 5804 R, Eppendorf, Hamburg, Germany) at 8,000 g and 4°C for 10 min. The bacteriocin-containing SPP was prepared by adding ammonium sulphate at 80% saturation to the cell-free supernatant to precipitate proteins with gentle stirring overnight at 4°C (Butkhot et al, 2019a). Subsequently, the precipitated protein was centrifuged at 10,000 g and 4°C for 30 min, and then resuspended in 50 mM sodium phosphate buffer (pH 7.0) prior to dialysis using a dialysis membrane tubing (1 kDa cut-off; Spectrum Laboratory, Los Angeles, CA, USA) at 4°C overnight.…”
Section: Cultivation Conditions and Preparation Of Bacteriocin From B...mentioning
confidence: 99%
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