New perspectives on the causes and consequences of male meiotic drive

Courret, Cécile; Wei, Xiaolu; Larracuente, Amanda M

doi:10.1016/j.gde.2023.102111

Cited by 5 publications

(1 citation statement)

References 48 publications

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“…These latter two features are important for several proposed methods of population suppression 13,21,42 . A number of naturally occurring gametic drive systems (most often biasing sperm genotypes) in animals have been characterized, but the information available does not yet provide guidance as to if or how they can be used as tools 43,44 . Engineering ClvR -based gametic drive in animals is challenging for several reasons.…”

Section: Mainmentioning

confidence: 99%

Cleave and Rescue gamete killers create conditions for gene drive in plants

Oberhofer,

Johnson,

Ivy

et al. 2023

Preprint

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Gene drive elements promote the spread of linked traits, even when their presence confers a fitness cost to carriers, and can be used to change the composition or fate of wild populations. Cleave and Rescue (ClvR) drive elements sit at a fixed chromosomal position and include a DNA sequence-modifying enzyme such as Cas9/gRNAs (the Cleaver/Toxin) that disrupts endogenous versions of an essential gene, and a recoded version of the essential gene resistant to cleavage (the Rescue/Antidote).ClvRspreads by creating conditions in which those lackingClvRdie because they lack functional versions of the essential gene. We demonstrate the essential features ofClvRgene drive in the plantArabidopsis thalianathrough killing of gametes that fail to inherit aClvRthat targets the essential gene YKT61, whose expression is required in male and female gametes for their survival. Resistant (uncleavable but functional) alleles, which can slow or prevent drive, were not observed. Modeling shows plantClvRs can be used to rapidly drive population modification or suppression. Possible applications in weed control, plant breeding and conservation are discussed.

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Section: Mainmentioning

confidence: 99%

Cleave and Rescue gamete killers create conditions for gene drive in plants

Oberhofer,

Johnson,

Ivy

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Cleave and Rescue gamete killers create conditions for gene drive in plants

Oberhofer,

Johnson,

Ivy

et al. 2024

Nat. Plants

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Running the gauntlet: challenges to genome integrity in spermiogenesis

Kitaoka,

Yamashita

2024

Nucleus

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Species’ continuity depends on gametogenesis to produce the only cell types that can transmit genetic information across generations. Spermiogenesis, which encompasses post-meiotic, haploid stages of male gametogenesis, is a process that leads to the formation of sperm cells well-known for their motility. Spermiogenesis faces three major challenges. First, after two rounds of meiotic divisions, the genome lacks repair templates (no sister chromatids, no homologous chromosomes), making it incredibly vulnerable to any genomic insults over an extended time (typically days-weeks). Second, the sperm genome becomes transcriptionally silent, making it difficult to respond to new perturbations as spermiogenesis progresses. Third, the histone-to-protamine transition, which is essential to package the sperm genome, counterintuitively involves DNA break formation. How spermiogenesis handles these challenges remains poorly understood. In this review, we discuss each challenge and their intersection with the biology of protamines. Finally, we discuss the implication of protamines in the process of evolution.

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New perspectives on the causes and consequences of male meiotic drive

Cited by 5 publications

References 48 publications

Cleave and Rescue gamete killers create conditions for gene drive in plants

Cleave and Rescue gamete killers create conditions for gene drive in plants

Cleave and Rescue gamete killers create conditions for gene drive in plants

Running the gauntlet: challenges to genome integrity in spermiogenesis

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