New physical analysis capability for counterfeit electronics and reverse engineering

Johnson, G.; Hartfield, Cheryl; Mueller, Sascha; Kaestner, Marcus

doi:10.1109/paine49178.2020.9337737

Cited by 6 publications

(4 citation statements)

References 8 publications

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“…Even for ROI targets that are significantly smaller than the grid spacing, their precise locations can be effectively communicated by describing micron-scale distances, measurable from the XRM data, to the sides or corners of any given grid cell. While similar approaches using XRM alone 24,25,32 or in combination with laser-patterned rulers to target subsurface features in bulk materials have been reported before 33 , our approach with a laser-patterned coordinate grid offers a much more comprehensive communication system through its full-sample coverage and alphanumerically labelled axes. The laser ablation process described herein is efficient, requiring minimal ablation time of 80 seconds in this case, and can be tailored to accommodate various grid spacings, rotations, and specimen shapes within the 40 × 40 mm LaserFIB scan field.…”

Section: A Fs Laser-patterned Coordinate Grid To Assist the Linking O...mentioning

confidence: 84%

Characterization of human trabecular bone across multiple length scales: A correlative approach combining X-ray tomography with LaserFIB and plasma FIB-SEM

Tang,

Casagrande,

Mohammadpour

et al. 2024

Preprint

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Three-dimensional correlative multimodal and multiscale imaging is an emerging method for investigating the complex hierarchical structure of biological materials such as bone. This approach synthesizes images acquired across multiple length scales, for the same region of interest, to provide a comprehensive view of the material structure of a sample. Here, we develop a workflow for the structural analysis of human trabecular bone using a femtosecond laser to produce a precise grid to facilitate correlation between imaging modalities and identification of structures of interest, in this case, a single trabecula within a volume of trabecular bone. Through such image registration, high resolution X-ray microscopy imaging revealed fine architectural details, including the cement line and bone cell lacunae of the selected bone unit. The selected bone volume was exposed with a combination of manual polishing and site-specific femtosecond laser ablation and then examined with plasma focused ion beam-scanning electron microscopy. This reliable and versatile correlation approach has the potential to be applied to a variety of biological tissues and traditional engineered materials. The proposed workflow has the enhanced capability for generating highly resolved structural data for a better understanding of the architectural features of a material spanning its macroscopic to nanoscopic levels.

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Section: A Fs Laser-patterned Coordinate Grid To Assist the Linking O...mentioning

confidence: 84%

Characterization of human trabecular bone across multiple length scales: A correlative approach combining X-ray tomography with LaserFIB and plasma FIB-SEM

Tang,

Casagrande,

Mohammadpour

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…The geometry of the carved specimen can be arbitrarily defined by the operator, including curved surfaces, making it trivial to generate samples in the shape of cylindrical pillars, a feature also at reach when milling the samples using lathe systems 2 but not when using microtome-based approaches. Fs lasers enable high ablation rates of up to several 10 6 µm 3 /s, depending on sample material and the laser settings used, and are compatible with heavy metal-stained, resin-embedded biological soft tissues 3,4 .…”

Section: Introduction and Resultsmentioning

confidence: 99%

“…Thus, we wanted to assess whether fs laser milling could be used to create several different targeted subvolumes from one single tissue specimen. To achieve this, we employed another fixed and stained olfactory bulb slice, mechanically trimmed to a ∼(1 mm) 3 cube ( Fig. 4a ).…”

Section: Introduction and Resultsmentioning

confidence: 99%

Femtosecond laser preparation of resin embedded samples for correlative microscopy workflows in life sciences

Bosch

Lindenau

Pacureanu

et al. 2023

Preprint

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Correlative multimodal imaging is a useful approach to investigate complex structural relations in life sciences across multiple scales. For these experiments, sample preparation workflows that are compatible with multiple imaging techniques must be established. In one such implementation, a fluorescently-labelled region of interest in a biological soft tissue sample can be imaged with light microscopy before staining the specimen with heavy metals, enabling follow-up higher resolution structural imaging at the targeted location, bringing context where it is required. Alternatively, or in addition to fluorescence imaging, other microscopy methods such as synchrotron X-ray computed tomography with propagation-based phase contrast (SXRT) or serial blockface scanning electron microscopy (SBF-SEM) might also be applied. When combining imaging techniques across scales, it is common that a volumetric region of interest (ROI) needs to be carved from the total sample volume before high resolution imaging with a subsequent technique can be performed. In these situations, the overall success of the correlative workflow depends on the precise targeting of the ROI and the trimming of the sample down to a suitable dimension and geometry for downstream imaging. Here we showcase the utility of a novel femtosecond laser device to prepare microscopic samples (1) of an optimised geometry for synchrotron X-ray microscopy as well as (2) for subsequent volume electron microscopy applications, embedded in a wider correlative multimodal imaging workflow (Fig. 1).

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“…S1 of the supplementary material), depending on the sample material and the laser settings used, and are compatible with heavy metalstained, resin-embedded biological soft tissues. 7,8 Altogether, these characteristics make the fs laser a suitable tool to extract targeted volumes of interest from a larger sample, the size, coordinates, and geometry of which were previously defined with compatible imaging techniques. Moreover, since the milling operation only requires access to one face of the resin block, multiple milling operations can be performed on a single specimen, enabling the extraction of tens of samples from neighboring regions in the same specimen.…”

mentioning

confidence: 99%

Femtosecond laser preparation of resin embedded samples for correlative microscopy workflows in life sciences

Bosch

Lindenau

Pacureanu

et al. 2023

Applied Physics Letters

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Correlative multimodal imaging is a useful approach to investigate complex structural relations in life sciences across multiple scales. For these experiments, sample preparation workflows that are compatible with multiple imaging techniques must be established. In one such implementation, a fluorescently labeled region of interest in a biological soft tissue sample can be imaged with light microscopy before staining the specimen with heavy metals, enabling follow-up higher resolution structural imaging at the targeted location, bringing context where it is required. Alternatively, or in addition to fluorescence imaging, other microscopy methods, such as synchrotron x-ray computed tomography with propagation-based phase contrast or serial blockface scanning electron microscopy, might also be applied. When combining imaging techniques across scales, it is common that a volumetric region of interest (ROI) needs to be carved from the total sample volume before high resolution imaging with a subsequent technique can be performed. In these situations, the overall success of the correlative workflow depends on the precise targeting of the ROI and the trimming of the sample down to a suitable dimension and geometry for downstream imaging. Here, we showcase the utility of a femtosecond laser (fs laser) device to prepare microscopic samples (1) of an optimized geometry for synchrotron x-ray tomography as well as (2) for volume electron microscopy applications and compatible with correlative multimodal imaging workflows that link both imaging modalities.

show abstract

New physical analysis capability for counterfeit electronics and reverse engineering

Cited by 6 publications

References 8 publications

Characterization of human trabecular bone across multiple length scales: A correlative approach combining X-ray tomography with LaserFIB and plasma FIB-SEM

Characterization of human trabecular bone across multiple length scales: A correlative approach combining X-ray tomography with LaserFIB and plasma FIB-SEM

Femtosecond laser preparation of resin embedded samples for correlative microscopy workflows in life sciences

Femtosecond laser preparation of resin embedded samples for correlative microscopy workflows in life sciences

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