New possibilities of enzymic synthesis of radioactive nucleotides. I. Synthesis of radioactive nucleoside‐5′‐triphosphates by an enzyme of Escherichia coli B

Abstract

Possibilities of enzymic synthesis of nucleoside-.5'-triphosphates- 14CC(U) of adenine, guanine, cytosine and uracil of high spec@ activity and radiochemical purity by means of a purijied enzyme isolatedfrom Escherichia coli B were studied. The efect of enzyme concentration in the reaction mixture in dependence on the time of the enzyme reaction is decisive for an optimal production of radioactive nucleoside-5'-triphosphates. A simple and rapid method of preparation of radioactive nucleoside-5'-triphosphates, including preparative paper chromatography as the single isolation step, is described and information on the radiolysis of the isolated nucleotides is presented.INTRODUCTION.

Review: Self‐radiolysis of compounds containing tritium and carbon‐14

“…In all cases, deoxynucleotides are more stable than their parent compounds (i.e., deoxyguanosine compared with guanosine), and phosphorolated nucleotide salts (Table 19) are more stable than their nonphosphorylated parent compounds (Table 18). Nejedlŷ et al report tri‐phosphate analogues of [U‐ 14 C]adenosine, guanosine, uridine, and cytidine undergo radiolytic dephosphorylation to afford diphosphates and, in the case of [U‐ 14 C]uridine triphosphate, small quantities of monophosphate 120 …”