Biotechnology for lignocellulose conversion is developed with its research and application. During this process, the research method plays an important role in discovering, exploring, and establishing theories. The research method for lignocellulose conversion is also necessary for industrialization testing and control. In this chapter, research methods for the biotechnology of lignocellulose are presented. According to the biological conversion process of lignocelluloses, research methods are classified into three types: research methods for primary refining, research methods for hydrolysis with microorganisms, and research methods for fermentation engineering. Finally, research methods for the whole biotransformation process are also summarized. In each part, the related research advances of my group are also given.
Keywords Research methods • Biochemical methods • Fermentation engineering methods • Primary refining methods • Life cycle assessment
Research Methods for the Cellulose Primary Refining Process
IntroductionCellulose materials should be converted into middle products first and then converted into final bioenergy and biomaterials. However, for cellulose materials, the compact structure formed by cellulose, hemicellulose, and lignin results in high energy cost while converting cellulose materials into sugar. Moreover, it is hard to fulfill the requirements of further conversion into bioenergy and biomaterials. Therefore, it would be important to research the changes of lignocellulosic materials in the process of primary conversion and the effect of different methods on these changes. This way, the recalcitrance of hydrolysis would be solved, and refining technology would be set up for the conversion of different materials into related products [1]. The recalcitrance of lignocellulosic materials includes two aspects: structure and components. Currently, characterization methods for lignocellulosic material components are usually from the papermaking area. In addition, near-infrared, X-ray diffraction, and electrical scanning technologies are applied to analyze the crystal structure and surface characteristics of cellulose materials. However, advanced characterization technology should be introduced to form a complete characterization technology system for deep and realistic recognition from different levels and aspects.Cross-discipline examination and re-creation are necessary to overcome difficult problems in the sciences. Now, discoveries are usually from a visual description or modeling. So, the scientific rules should be followed to find the recalcitrance of cellulose materials in the primary refining process: from visual to abstract, from macro to micro, from outside to inside. In this section, the research methods are introduced gradually according to this order.At first, the morphological and structural changes should be characterized because it could provide direct recognition. Especially, different changes could be observed in the process of primary refining, including special structur...