New Psychoactive Substance 5-MeO-MIPT In vivo Acute Toxicity and Hystotoxicological Study

Altuncı, Yusuf Ali; Aydoğdu, Melike; Açıkgöz, Eda; Güven, Ümmü; Duzağa, Fahriye; Atasoy, Aslı A.; Dağlioğlu, Nebīle; Akgür, Serap Annette

doi:10.4274/balkanmedj.galenos.2020.2019.11.68

Cited by 3 publications

(2 citation statements)

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“…In this presented study, a validated method was developed after chemometrically optimization for the detection of 5-MeO-MiPT in urine samples using GC-MS. Regarding tryptamine derivatives, in vitro and in vivo metabolism studies (1,15), hallucination and acute effect studies in mice and rats (16,17), intoxication case reports (18,19), real case urine, hair and blood sample studies (6,(20)(21)(22)(23)(24) are included in the literature. In the metabolism study was about 5-MeO-MiPT in 2017, the obtained blood and urine samples were analysed for in vivo metabolites of 5-MeO-MiPT using liquid chromatography-high resolution tandem mass spectrometry (1).…”

Section: Discussionmentioning

confidence: 99%

Halüsinasyon yapan yeni tasarım psikoaktif maddelerden biri olan 5-MeO-MiPTin idrarda tayinine yönelik kemometrik yöntem geliştirilmesi

EMEN,

ASLAN,

AYDOĞDU

et al. 2023

Ege Tıp Dergisi

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Aim: In this study, a method was developed for analysis and chemometric optimization for 5-methoxy-N-methyl-N-isopropyltryptamine (5-MeO-MiPT). Materials and Methods: Our study was carried out in Ege University Institute on Drug Abuse, Toxicology and Pharmaceutical Science, Addiction Toxicology Laboratory. Analysis and optimization of the effects of hydrolysis and solid phase extraction processes during the analysis of 5-MeO-MiPT by Gas Chromatography-Mass Spectrometry (GC-MS). For chemometric screenings design, Plackett-Burman was used. The most effective three factors were determined, and a central composite design was applied, and results were evaluated with surface response methodology. The method was validated for selectivity, linearity, the limit of detection, the limit of quantitation, accuracy, intra-day and inter-day repeatability, stability and carry over. Results: In the chemometric method, the most effective parameters were found sample volume, hydrolysis temperature, and elution volume for 5-MeO-MiPT in urine analysis. Optimum values for these parameters were calculated by surface response methodology and the results were determined 1ml urine volume, 30°C hydrolysis temperature, 3,5 ml elution volume, respectively. The optimized method was validated for selectivity, linearity (25-500 ng/mL), limit of detection (5 ng/mL), limit of quantitation (18 ng/mL), accuracy (72-101%), intra-day and inter-day precisions were measured, respectively (4,43% RSD),(4,27% CV), stability and carry over parameters. Conclusion: With a chemometric approach, quick, practical and accurate method for the detection of 5-MeO-MiPT has been developed with GC-MS. Working of 5-MeO-MiPT without derivatization in GC-MS analysis has shortened the pre-preparation time and is a pioneer for other analogs. It provides an effective method in the analysis of substances such as synthetic analogues from tryptamines which are added every day, with the use of such classical equipment and new methods.

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Section: Discussionmentioning

confidence: 99%

Halüsinasyon yapan yeni tasarım psikoaktif maddelerden biri olan 5-MeO-MiPTin idrarda tayinine yönelik kemometrik yöntem geliştirilmesi

EMEN,

ASLAN,

AYDOĞDU

et al. 2023

Ege Tıp Dergisi

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“…The toxicity is still relatively unknown, but recent research showed evidence of acute toxicity in mice when given a high dose. 5-MeO-MiPT is still uncontrolled in large parts of the world (Altuncı, et al, 2021;Repke, et al, 1985).…”

Section: Stimulant Name Descriptionmentioning

confidence: 99%

Finding unknown stimulants applied in food supplements using Artificial Intelligence

Gavai¹,

Bouzembrak²,

Bulk³

et al. 2021

Preprint

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The world market for food supplements is large and is driven by the claims of these products to, for example, treat obesity, increase focus and alertness, decrease appetite, decrease the need for sleep or reduce impulsivity. The use of illegal compounds in food supplements is a continuous threat, certainly because these compounds and products have not been tested for safety by competent authorities. It is therefore of the utmost importance for the competent authorities to know when new products are being marketed and to warn users against potential health risks. In this study we present an approach to detect new and unknown stimulants in food supplements using machine learning. More than 20 new stimulants were identified from two different data sources, namely scientific literature applying word embedding on > 2 million abstracts and articles from formal and social media on the world wide web using text mining. The results show that the developed approach may be suitable to detect “unknowns” in the emerging risk identification activities performed by the competent authorities, which is currently a major hurdle.

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Pharmaco-toxicological effects of the novel tryptamine hallucinogen 5-MeO-MiPT on motor, sensorimotor, physiological, and cardiorespiratory parameters in mice—from a human poisoning case to the preclinical evidence

Bassi,

Bilel,

Tirri

et al. 2024

Psychopharmacology

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Rationale The 5-methoxy-N-methyl-N-isopropyltryptamine (5-MeO-MiPT, known online as “Moxy”) is a new psychedelic tryptamine first identified on Italian national territory in 2014. Its hallucinogen effects are broadly well-known; however, only few information is available regarding its pharmaco-toxicological effects. Objectives Following the seizure of this new psychoactive substances by the Arm of Carabinieri and the occurrence of a human intoxication case, in the current study we had the aim to characterize the in vivo acute effects of systemic administration of 5-MeO-MiPT (0.01–30 mg/kg i.p.) on sensorimotor (visual, acoustic, and overall tactile) responses, thermoregulation, and stimulated motor activity (drag and accelerod test) in CD-1 male mice. We also evaluated variation on sensory gating (PPI, prepulse inhibition; 0.01–10 mg/kg i.p.) and on cardiorespiratory parameters (MouseOx and BP-2000; 30 mg/kg i.p.). Lastly, we investigated the in silico ADMET (absorption, distribution, metabolism, excretion, toxicity) profile of 5-MeO-MiPT compared to 5-methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT) and N,N-dimethyltryptamine (DMT). Results This study demonstrates that 5-MeO-MiPT dose-dependently inhibits sensorimotor and PPI responses and, at high doses, induces impairment of the stimulated motor activity and cardiorespiratory changes in mice. In silico prediction shows that the 5-MeO-MiPT toxicokinetic profile shares similarities with 5-MeO-DIPT and DMT and highlights a cytochrome risk associated with this compound. Conclusions Consumption of 5-MeO-MiPT can affect the ability to perform activities and pose a risk to human health status, as the correspondence between the effects induced in mice and the symptoms occurred in the intoxication case suggests. However, our findings suggest that 5-MeO-MiPT should not be excluded from research in the psychiatric therapy field.

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New Psychoactive Substance 5-MeO-MIPT In vivo Acute Toxicity and Hystotoxicological Study

Cited by 3 publications

References 24 publications

Halüsinasyon yapan yeni tasarım psikoaktif maddelerden biri olan 5-MeO-MiPTin idrarda tayinine yönelik kemometrik yöntem geliştirilmesi

Halüsinasyon yapan yeni tasarım psikoaktif maddelerden biri olan 5-MeO-MiPTin idrarda tayinine yönelik kemometrik yöntem geliştirilmesi

Finding unknown stimulants applied in food supplements using Artificial Intelligence

Pharmaco-toxicological effects of the novel tryptamine hallucinogen 5-MeO-MiPT on motor, sensorimotor, physiological, and cardiorespiratory parameters in mice—from a human poisoning case to the preclinical evidence

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