New rabbit knee model of posttraumatic joint contracture: Indirect capsular damage induces a severe contracture

Nesterenko, Sergiy; Morrey, Mark E.; Abdel, Matthew P.; An, Kai Nan; Steinmann, Scott P.; Morrey, B.F.; Sánchez-Sotelo, Joaquín

doi:10.1002/jor.20845

Cited by 62 publications

(130 citation statements)

References 19 publications

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“…Rabbits assigned to the arthrofibrosis model underwent the same initial procedure to develop a contracture as described in previous studies and briefly outlined below. 5,12-14,33 Collagen scaffolds used in this study were commercially available collagen sponges, derived from purified bovine tendon, currently used in dental applications. These scaffolds are pre-sterilised, eliminating the need for sterilisation.…”

Section: Methodsmentioning

confidence: 99%

“…1,2,3 In order to study the pathophysiology behind arthrofibrosis, animal models have been developed using the New Zealand White rabbit knee. 4,5 These models involve an intracapsular knee injury followed by Kirschner-wire (K-wire) immobilisation for eight weeks, and result in substantial contracture that resembles the human biomechanical, cellular, and molecular response to injury, with increased myofibroblasts and transforming growth factor beta (TGF-β) activity. 6-12 …”

Section: Introductionmentioning

confidence: 99%

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Intra-articular implantation of collagen scaffold carriers is safe in both native and arthrofibrotic rabbit knee joints

Walker

Ewald

Lewallen

et al. 2017

Bone & Joint Research

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ObjectivesSustained intra-articular delivery of pharmacological agents is an attractive modality but requires use of a safe carrier that would not induce cartilage damage or fibrosis. Collagen scaffolds are widely available and could be used intra-articularly, but no investigation has looked at the safety of collagen scaffolds within synovial joints. The aim of this study was to determine the safety of collagen scaffold implantation in a validated in vivo animal model of knee arthrofibrosis.Materials and MethodsA total of 96 rabbits were randomly and equally assigned to four different groups: arthrotomy alone; arthrotomy and collagen scaffold placement; contracture surgery; and contracture surgery and collagen scaffold placement. Animals were killed in equal numbers at 72 hours, two weeks, eight weeks, and 24 weeks. Joint contracture was measured, and cartilage and synovial samples underwent histological analysis.ResultsAnimals that underwent arthrotomy had equivalent joint contractures regardless of scaffold implantation (-13.9° versus -10.9°, equivalence limit 15°). Animals that underwent surgery to induce contracture did not demonstrate equivalent joint contractures with (41.8°) or without (53.9°) collagen scaffold implantation. Chondral damage occurred in similar rates with (11 of 48) and without (nine of 48) scaffold implantation. No significant difference in synovitis was noted between groups. Absorption of the collagen scaffold occurred within eight weeks in all animalsConclusionOur data suggest that intra-articular implantation of a collagen sponge does not induce synovitis or cartilage damage. Implantation in a native joint does not seem to induce contracture. Implantation of the collagen sponge in a rabbit knee model of contracture may decrease the severity of the contracture.Cite this article: J. A. Walker, T. J. Ewald, E. Lewallen, A. Van Wijnen, A. D. Hanssen, B. F. Morrey, M. E. Morrey, M. P. Abdel, J. Sanchez-Sotelo. Intra-articular implantation of collagen scaffold carriers is safe in both native and arthrofibrotic rabbit knee joints. Bone Joint Res 2016;6:162–171. DOI: 10.1302/2046-3758.63.BJR-2016-0193.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Intra-articular implantation of collagen scaffold carriers is safe in both native and arthrofibrotic rabbit knee joints

Walker

Ewald

Lewallen

et al. 2017

Bone & Joint Research

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“…Contracture can occur when a muscle undergoes disuse, as in the case of limb immobilization [8]. The technique of fixation of muscles at abnormally short lengths can be used to study muscle atrophy [7,9,10].…”

Section: Introductionmentioning

confidence: 99%

Contributions of biarticular myogenic components to the limitation of the range of motion after immobilization of rat knee joint

Nagai

Aoyama

Ito

et al. 2014

BMC Musculoskelet Disord

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BackgroundMuscle atrophy caused by immobilization in the shortened position is characterized by a decrease in the size or cross-sectional area (CSA) of myofibers and decreased muscle length. Few studies have addressed the relationship between limitation of the range of motion (ROM) and the changes in CSA specifically in biarticular muscles after atrophy because of immobilization. We aimed to determine the contribution of 2 distinct muscle groups, the biarticular muscles of the post thigh (PT) and those of the post leg (PL), to the limitation of ROM as well as changes in the myofiber CSAs after joint immobilization surgery.MethodsMale Wistar rats (n = 40) were randomly divided into experimental and control groups. In the experimental group, the left knee was surgically immobilized by external fixation for 1, 2, 4, 8, or 16 weeks (n = 5 each) and sham surgery was performed on the right knee. The rats in the control groups (n = 3 per time point) did not undergo surgery. After the indicated immobilization periods, myotomy of the PT or PL biarticular muscles was performed and the ROM was measured. The hamstrings and gastrocnemius muscles from the animals operated for 1 or 16 weeks were subjected to morphological analysis.ResultsIn immobilized knees, the relative contribution of the PT biarticular myogenic components to the total restriction reached 80% throughout the first 4 weeks and decreased thereafter. The relative contribution of the PL biarticular myogenic components remained <20% throughout the immobilization period. The ratio of the myofiber CSA of the immobilized to that of the sham-operated knees was significantly lower at 16 weeks after surgery than at 1 week after surgery only in the hamstrings.ConclusionsThe relative contribution of the PT and PL components to myogenic contracture did not significantly change during the experimental period. However, the ratio of hamstrings CSAs to the sham side was larger than the ratio of medial gastrocnemius CSAs to the sham side after complete atrophy because of immobilization.

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“…An extension torque was then applied to the tibia through a pulley at 1° per second to a maximum torque of 20 Ncm. 7 This device measures the flexion contracture of the limb, and is calibrated using standardised weights. It has been documented in the literature by our group.…”

Section: Methodsmentioning

confidence: 99%

“…1,2 An animal model has been recently developed and used to simulate joint contractures. 3-7 This model mimics the biomechanical and histological presentation of clinical contracture. 5,7-11 …”

Section: Introductionmentioning

confidence: 99%

Effectiveness of rosiglitazone in reducing flexion contracture in a rabbit model of arthrofibrosis with surgical capsular release

Barlow¹,

Morrey

Hartzler³

et al. 2016

Bone & Joint Research

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AimsAnimal models have been developed that allow simulation of post-traumatic joint contracture. One such model involves contracture-forming surgery followed by surgical capsular release. This model allows testing of antifibrotic agents, such as rosiglitazone.MethodsA total of 20 rabbits underwent contracture-forming surgery. Eight weeks later, the animals underwent a surgical capsular release. Ten animals received rosiglitazone (intramuscular initially, then orally). The animals were sacrificed following 16 weeks of free cage mobilisation. The joints were tested biomechanically, and the posterior capsule was assessed histologically and via genetic microarray analysis.ResultsThere was no significant difference in post-traumatic contracture between the rosiglitazone and control groups (33° (standard deviation (sd) 11) vs 37° (sd14), respectively; p = 0.4). There was no difference in number or percentage of myofibroblasts. Importantly, there were ten genes and 17 pathways that were significantly modulated by rosiglitazone in the posterior capsule.DiscussionRosiglitazone significantly altered the genetic expression of the posterior capsular tissue in a rabbit model, with ten genes and 17 pathways demonstrating significant modulation. However, there was no significant effect on biomechanical or histological properties.Cite this article: M. P. Abdel. Effectiveness of rosiglitazone in reducing flexion contracture in a rabbit model of arthrofibrosis with surgical capsular release: A biomechanical, histological, and genetic analysis. Bone Joint Res 2016;5:11–17. DOI: 10.1302/2046-3758.51.2000593

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New rabbit knee model of posttraumatic joint contracture: Indirect capsular damage induces a severe contracture

Cited by 62 publications

References 19 publications

Intra-articular implantation of collagen scaffold carriers is safe in both native and arthrofibrotic rabbit knee joints

Intra-articular implantation of collagen scaffold carriers is safe in both native and arthrofibrotic rabbit knee joints

Contributions of biarticular myogenic components to the limitation of the range of motion after immobilization of rat knee joint

Effectiveness of rosiglitazone in reducing flexion contracture in a rabbit model of arthrofibrosis with surgical capsular release

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