New Rapid HPLC Method for Separation and Determination of Benzo[<i>A</i>]Pyrene Hydroxyderivatives

Bednáriková, Alena; Skláršová, Božena; Kolek, Emil; Polovka, Martin; Šimko, Peter

doi:10.1080/10406638.2011.621505

Cited by 10 publications

(2 citation statements)

References 42 publications

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“…After 6 hours at 37 °C and 5% of CO 2 , the medium was collected and centrifuged 15 min at 20,000 g at 4 °C, and 50 µL of the supernatant was directly injected into the HPLC system. The HPLC analysis was performed with the Agilent 1100TM system equipped with an Accucore PFP column (150 mm × 3 mm, particle size 2.6 µm) coupled with a fluorescent detector, as used by others for B[a]P metabolite detection 40 – 42 . A gradient of 0.1% acetic acid and acetonitrile was used throughout the experiment at a flow rate of 0.650 mL/min.…”

Section: Methodsmentioning

confidence: 99%

Co-exposure to benzo[a]pyrene and ethanol induces a pathological progression of liver steatosis in vitro and in vivo

Bucher

Tête

Podechard

et al. 2018

Sci Rep

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Hepatic steatosis (i.e. lipid accumulation) and steatohepatitis have been related to diverse etiologic factors, including alcohol, obesity, environmental pollutants. However, no study has so far analyzed how these different factors might interplay regarding the progression of liver diseases. The impact of the co-exposure to the environmental carcinogen benzo[a]pyrene (B[a]P) and the lifestyle-related hepatotoxicant ethanol, was thus tested on in vitro models of steatosis (human HepaRG cell line; hybrid human/rat WIF-B9 cell line), and on an in vivo model (obese zebrafish larvae). Steatosis was induced prior to chronic treatments (14, 5 or 7 days for HepaRG, WIF-B9 or zebrafish, respectively). Toxicity and inflammation were analyzed in all models; the impact of steatosis and ethanol towards B[a]P metabolism was studied in HepaRG cells. Cytotoxicity and expression of inflammation markers upon co-exposure were increased in all steatotic models, compared to non steatotic counterparts. A change of B[a]P metabolism with a decrease in detoxification was detected in HepaRG cells under these conditions. A prior steatosis therefore enhanced the toxicity of B[a]P/ethanol co-exposure in vitro and in vivo; such a co-exposure might favor the appearance of a steatohepatitis-like state, with the development of inflammation. These deleterious effects could be partly explained by B[a]P metabolism alterations.

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Section: Methodsmentioning

confidence: 99%

Co-exposure to benzo[a]pyrene and ethanol induces a pathological progression of liver steatosis in vitro and in vivo

Bucher

Tête

Podechard

et al. 2018

Sci Rep

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show abstract

“…Various analytical techniques such as GC, GC-MS, HPLC-FLD, and HPLC-MS have been applied to benzo[a]pyrene analysis, and the advantages and disadvantages of these methods have been thoroughly discussed in many papers (18)(19)(20)(21).…”

Section: Introductionmentioning

confidence: 99%

Determination of benzo[a]pyrene in camellia oil via vortex-assisted extraction using the UPLC-FLD method

Zhang

et al. 2017

Food Sci Biotechnol

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In this paper, vortex-assisted extraction using the ultraperformance liquid chromatography analysis method was performed to determine benzo[a]pyrene in camellia oil. Optimum results were obtained when 0.5 g of oil sample was used followed by vortex-assisted extraction for 10 min with 25 mL of acetonitrile. Chromatographic separation was performed on an Agilent ZORBAX Eclipse Plus C column (2.1mm×100mm, particle size 1.8 μm). The optimum mobile phase comprised 70% acetone and 30% water. The detection limit of benzo[a]pyrene was 0.2 μg/kg. The recoveries were in the range of 81.0-97.0%. The proposed method was simple and fast, and it provided high throughput in the determination of benzo[a]pyrene in an oil matrix sample.

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Factors Affecting Elimination of Carcinogenic Compounds from Food Products

Šimko

2015

Food Engineering Series

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New Rapid HPLC Method for Separation and Determination of Benzo[A]Pyrene Hydroxyderivatives

Cited by 10 publications

References 42 publications

Co-exposure to benzo[a]pyrene and ethanol induces a pathological progression of liver steatosis in vitro and in vivo

Co-exposure to benzo[a]pyrene and ethanol induces a pathological progression of liver steatosis in vitro and in vivo

Determination of benzo[a]pyrene in camellia oil via vortex-assisted extraction using the UPLC-FLD method

Factors Affecting Elimination of Carcinogenic Compounds from Food Products

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