New Rapid Polymerase Chain Reaction‐Immunochromatographic Assay for <i>Porphyromonas gingivalis</i>

Takada, Kazuko; Sakaguchi, Yutaku; Oka, Chitoshi; Hirasawa, Masatomo

doi:10.1902/jop.2005.76.4.508

Cited by 13 publications

(10 citation statements)

References 26 publications

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“…To our knowledge, this is the first time that this combination of PCR and immunochromatographic one-step detection is described for Listeria. The combination of both techniques into an assay for rapid detection of specific nucleic acid targets was already described for detection of the mecA gene from methicillin-resistant Staphylococcus aureus (MRSA) cultures [29], for direct diagnosis of Porphyromonas gingivalis [30], and for the identification of Mycobacterium tuberculosis [31].…”

Section: Development Of Nalfia For Listeria Detectionmentioning

confidence: 99%

Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeria monocytogenes in food

Blažková

Koets

Rauch

et al. 2009

Eur Food Res Technol

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We present a new nucleic acid lateral flow immunoassay (NALFIA) for the assessment of listeria contamination. The detection procedure starts with enrichment of sample in Half Fraser broth (24 h). Following isolation of DNA, a duplex PCR is performed with two labelled primer sets, one generic and directed to a specific sequence of the gene encoding 16S rRNA from Listeria spp. and the other specific and directed to a part of the prfA gene encoding the central virulence gene regulator from the food pathogen Listeria monocytogenes (3.5 h). The PCR solution is directly added to the one-step assay device and the appearance of a grey/black line is indicative of the presence of specific amplicons (max 15 min). In all tests performed, the method correctly identified L. monocytogenes and strains of Listeria spp. PCR material of over 20 food samples was tested by NALFIA. The method proved to be useful for the detection of L. monocytogenes in different kinds of food samples.

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Section: Development Of Nalfia For Listeria Detectionmentioning

confidence: 99%

Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeria monocytogenes in food

Blažková

Koets

Rauch

et al. 2009

Eur Food Res Technol

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“…Analyte antigens in the sample bind to the antibodies and their presence or absence is then indicated by the extent of colour development at the test line as the labeled antibodies bind to it. During the last few years these lateral flow devices have been adapted for nucleic acid detection, [4][5][6] but because they are still based on haptens and antibodies they are unnecessarily complicated and expensive. In a typical example, nucleic acids are amplified with haptenylated primers and detected by sandwiching them between antibodies conjugated to GNPs and a nitrocellulose membrane striped with adsorbed antibodies (Fig.…”

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confidence: 99%

One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device

et al. 2007

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“…This method has been used for the rapid amplification and detection of pathogenic microorganisms in blood, sputum, and stool specimens from patients [Chow et al, 2008;Goldmeyer et al, 2008;Tang et al, 2010]. Takada et al [2005] reported a great potentiality of such technique using a similar sandwich immunoassay amplicon detection format for PCR assays on direct diagnosis of Porphyromonas gingivalis from periodontitis patients, and Soo et al [2006] developed PCR combined with immuno-chromatography test assay for detection of Mycobacterium tuberculosis.…”

Section: Resultsmentioning

confidence: 99%

Application of polymerase chain reaction with disposable amplicon detection device for identification of regulatory gene introduced into genetically modified maize

Woo

Chung

Shin

et al. 2011

J Korean Soc Appl Biol Chem

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A novel genetically modified organism (GMO) detection method was developed using a simple and rapid procedure to identify a regulatory gene introduced into genetically modified (GM) maize. After DNA extraction from GM maize flours, fragments of the conserved sequence of the cauliflower masaic virus (CaMV) 35S promoter gene along with a competitive internal control gene were amplified using duplex polymerase chain reaction (PCR). Amplification products were then detected using a disposable detection device. The quantitative detection limit for GM maize 59122 was found to be 1.0% (w/w). Because the combination-method involving PCR technology coupled with a disposable detection device does not require expensive instrumentation or expertise, it will serve as a valuable alternative to immunoassays and traditional PCR-based tests in the detection of GMOs.

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New Rapid Polymerase Chain Reaction‐Immunochromatographic Assay for Porphyromonas gingivalis

Abstract: A diagnostic assay based on PCR-ICA was developed for the detection of P. gingivalis, and results were obtained visually in 3 hours. PCR-ICA will be a valuable tool for the rapid detection of target bacteria by chair side.

Cited by 13 publications

References 26 publications

Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeria monocytogenes in food

Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeria monocytogenes in food

One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device

Application of polymerase chain reaction with disposable amplicon detection device for identification of regulatory gene introduced into genetically modified maize

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