New rat to mouse xenograft transplantation of endometrium as a model of human endometriosis

Abdolmaleki, Amir; Mansouri, Kamran; Bakhtiari, Mitra

doi:10.1002/ame2.12181

Cited by 8 publications

(3 citation statements)

References 27 publications

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“…MDA concentration was measured using the following formula; the amount of MDA (nM)/mg × 4* × D = nM/mg. 4* was the correction for using 200 μl of the 800 μl reaction mix 12 …”

Section: Methodsmentioning

confidence: 99%

Single intratesticular injection of blood‐serum‐derived exosomes can potentially alleviate testopathy following testicular torsion

Keivan

Torghabeh

Davoodi

et al. 2022

Anim Models and Exp Med

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The sexual system in men includes the testicles, seminal vesicles, and penis. The most important part of this system is the testicles, which produce sperm and the hormone testosterone. The testicles are located inside the sac and are protected by different layers. The structure of the testicles consists of the intricate tubes that make up sperm. The intermediate layers of the seminiferous tubules are

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“…MDA concentration was measured using the following formula; the amount of MDA (nM)/mg × 4* × D = nM/mg. 4* was the correction for using 200 μl of the 800 μl reaction mix 12 …”

Section: Methodsmentioning

confidence: 99%

Single intratesticular injection of blood‐serum‐derived exosomes can potentially alleviate testopathy following testicular torsion

Keivan

Torghabeh

Davoodi

et al. 2022

Anim Models and Exp Med

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“…Harris hematoxylin staining was used for counterstaining. On the basis of the published protocol of H&E staining, the histological sections were treated as follows: removal of paraffin wax using xylene, application of absolute alcohol for removal of xylene, application of alcohol with descending concentration for hydration, staining with colored solutions of hematoxylin and eosin, application of alcohol with ascending concentration for dehydration, and clarification of tissues with xylene 10 …”

Section: Methodsmentioning

confidence: 99%

Royal‐jelly‐based apitherapy can attenuate damages to male reproductive parameter following nicotine administration

Nazar-Zadeh

Jalili

Fathi

et al. 2022

Anim Models and Exp Med

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Background Nicotine administration can generate severe oxidative stress and lipid peroxidation. Royal jelly, with its antioxidant properties, acts as a scavenger of reactive oxygen species. This study describes the apitherapy effects of royal jelly on testicular damage following nicotine administration. Methods Forty‐eight male BALB/c mice were divided into 8 groups ( n = 6): saline, 3 different doses of royal jelly (100, 150, and 200 mg/kg body weight (BW) per day), nicotine (1.5 mg/kg), and 3 different groups of Nic + Roy (1.5 mg/kg of Nic + 100, 150, and 200 mg/kg BW per day of royal jelly). Nicotine was administrated intraperitoneally, and royal jelly was prescribed orally for 10 consecutive days. Serum levels of hormones (testosterone, luteinizing hormone, and follicle‐stimulating hormone), total antioxidant capacity, nitric oxide (NO) status, malondialdehyde levels, sperm DNA fragmentation, sperm parameters, histopathological changes (H&E staining), immunohistochemistry against apoptotic proteins, and gene expression of Bcl ‐ 2 , p53 , Caspase ‐ 3 , and Nrf2 (real‐time PCR) were assessed to evaluate the molecular and histological changes. Results Hormone levels, sperm parameters, and status of antioxidants were decreased significantly ( p < .05) following nicotine administration. Moreover, royal jelly treatment normalized hormonal and antioxidant characteristics, decreased apoptotic gene expression, increased Nfr2 gene expression, and restored histopathological alteration to the physiological status significantly ( p < .05). Conclusion Royal jelly upregulates the antioxidant status, inhibits the mitochondrial‐dependent apoptosis pathway, and increases the rate of proliferation. This therapeutic agent effectively protected the testis against nicotine‐associated damages by antioxidant and anti‐apoptotic effects.

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“…The animals were dissected and uterus examined for pregnancy according to standard procedures as described by [18].…”

Section: Pregnancy Statusmentioning

confidence: 99%

Effects of Hydro-ethanolic Leaf Extract of Sida acuta on Female Albino Rats’ Reproductive Potential and Weight: An Interventional Study

Ibeagi

Muhammed

Bamidele

et al. 2023

AJRB

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Background: Human fertility control (family planning) is among the major approaches through which unwanted pregnancy can be curbed. Hence, utilization of medicinal herbs in the development and formulation of products, as an alternative to conventional drugs, is one of the method options to achieve this. Aim: This study was designed to assess the contraceptive potentials of hydro-ethanolic leaf extract of Sida acuta, through evaluating its effects on fertility hormones, pregnancy status, and body weight of female albino rats. Method: This experiment was conducted using thirty five (35) sexually matured albino rats. Using a CRD, the rats were randomly divided into five (5) groups of seven (7) rats each, with five (5) female and two (2) male replicate assigned to each group. The Control group (Group 1) did not receive any treatment. The females in the standard group (Group 2) were administerd with levonorgestrel-etinyl estradiol (standard drug) 7.5/0.75mg/kg b.w./day p.o. The females in the test groups (Groups 3, 4 and 5) were administered the plant extract at different doses of 100mg/kg b.w./day (Low dose), 200mg/kg b.w./day (Medium dose) and 400mg/kg b.w./day (High dose) p.o. respectively, for 28days. The initial and final body weights of the animals were measured. The qualitative and quantitative phytochemical analyses of hydro-ethanolic leaf extract of Sida acuta, as well as the GC-MS analysis were also performed using standard methods. Furthermore, the effects of the extract were checked on the reproductive hormones (FSH, LH, Progesterone and Estrogen), pregnancy status as well as the body weight of the animals. Results: The results revealed the presence of bioactive compounds in the hydro-ethanolic leaf extract of Sida acuta which may be responsible for the contraceptive potentials of the plant. There were decreased levels of FSH across the groups. There was no pregnancy outcome in group 5 (high-dose group). And there was a significant weight reduction in group 5 (high-dose group). Conclusion: The result suggested that the hydro-ethanolic leaf extract of Sida acuta has suppressive effects on the reproductive hormones (especially the gonadotropins) at higher dose, thereby establishing its contraceptive potentials as confirmed by the pregnancy outcome. Also, the extract is capable of significantly decreasing body weight at a high dose.

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New rat to mouse xenograft transplantation of endometrium as a model of human endometriosis

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 8 publications

References 27 publications

Single intratesticular injection of blood‐serum‐derived exosomes can potentially alleviate testopathy following testicular torsion

Single intratesticular injection of blood‐serum‐derived exosomes can potentially alleviate testopathy following testicular torsion

Royal‐jelly‐based apitherapy can attenuate damages to male reproductive parameter following nicotine administration

Effects of Hydro-ethanolic Leaf Extract of Sida acuta on Female Albino Rats’ Reproductive Potential and Weight: An Interventional Study

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