New Reactive Fluorophores in the 1,2,3‐Triazine Series.

Butler, R. N.; Fahy, Aoife M.; Fox, Anthony; Stephens, John C.; Mcardle, P.; Cunnigham, D.; Ryder, Alan G.

doi:10.1002/chin.200626145

Cited by 1 publication

(1 citation statement)

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“…Measuring at low signal-to-noise ratios can adversely affect identification and localization of single molecules. Finally, a synthetic approach to investigate new fluorophores with improved lifetimes is another method aimed at better fluorophore options in the future [36-38], but does not offer an immediate solution for non-synthetic labs that depend on commercially available fluorophores. Also, fluorophores often require specific functionalization, such as N-hydroxysuccinimide or hydrazide, for labeling applications that may not be available with newly discovered fluorophores.…”

Section: Introductionmentioning

confidence: 99%

Extending single molecule fluorescence observation time by amplitude-modulated excitation

Kisley

Cooper

Mansur

et al. 2013

Methods Appl. Fluoresc.

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We present a hardware-based method that can improve single molecule fluorophore observation time by up to 1500% and super-localization by 47% for the experimental conditions used. The excitation was modulated using an acousto-optic modulator (AOM) synchronized to the data acquisition and inherent data conversion time of the detector. The observation time and precision in super-localization of four commonly used fluorophores were compared under modulated and traditional continuous excitation, including direct total internal reflectance excitation of Alexa 555 and Cy3, non-radiative Förster resonance energy transfer (FRET) excited Cy5, and direct epi-fluorescence wide field excitation of Rhodamine 6G. The proposed amplitude-modulated excitation does not perturb the chemical makeup of the system or sacrifice signal and is compatible with multiple types of fluorophores. Amplitude-modulated excitation has practical applications for any fluorescent study utilizing an instrumental setup with time-delayed detectors.

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Section: Introductionmentioning

confidence: 99%