New reagents for detection of faecal occult blood

Syed, Akheel Ahmed; Khatoon, Bibi Ahmadi; Silwadi, Mohammed Fawaz

doi:10.1016/s0731-7085(00)00454-4

Cited by 8 publications

(3 citation statements)

References 12 publications

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“…o-Toluidine and benzidine have both been used as alternatives to guaiac but have been discontinued because they have been shown to be to be carcinogenic [52]. Imipramine and desipramine have also been described as alternative reagents to guaiac and have reports of less interference from vegetable peroxidases, iron and vitamin C, but they have not gained a place in the market [116]. Alpha guaiaconic acid, the active component of guaiac gum, has been synthesised but proved unstable and unsuitable as an alternative to the tree extract, which may contain contaminants with stabilising properties.…”

Section: Other Testsmentioning

confidence: 99%

European guidelines for quality assurance in colorectal cancer screening and diagnosis. – First Edition Faecal occult blood testing

2012

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Section: Other Testsmentioning

confidence: 99%

European guidelines for quality assurance in colorectal cancer screening and diagnosis. – First Edition Faecal occult blood testing

2012

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“…[5] All diet and drug precautions were applied before obtaining samples to prevent false positive results as dietary restriction of both hemoglobin and vegetable peroxidase containing substances is essential for valid screening for occult blood. [6]…”

Section: Methodsmentioning

confidence: 99%

H Pylori Infection as Risk Factor for GIT Bleeding in Haemophilic Patients

2016

Internal Med Res Open J

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Background: Helicobacter pyelori is endemic in Egypt and present a main cause of gastrointestinal bleeding.Aim: Aim of this study is to evaluate the prevalence of Hpylori infection in hemophilic patients, and to assess its impact on gastrointestinal bleeding associated with this infection in such patients.Methods: we prospectively investigated the prevalence of H. pylori infection in 40 Egyptian patients with hemophilia A, B and von Willebrand syndrome and 20 normal male subjects were included. Every patient and control subject in the study were tested one time for H. pylori stool antigen by ELISA. All patients and control subjects were tested for occult blood using Guaiac-based fecal occult blood test.Results: Twenty eight out of 40 patients (70%) are H. pylori positive ; and 12 out of 20 control (60%) are H. pylori positive. The odds ratio is 1.55, 95% CI (0.6162 to 3.9269), Significance level P = 0.3497. Among 28 H.pylori positive patients, 5 patients (17.9%) tested positive for occult blood. Among the 12 H.pylori positive subjects in the control group, only one tested positive for occult blood (8.3%). Odds ratio for Occult bleeding in H pylori positive patients and control was 2.39: P= 0.4504. None of the H. pylori negative patients or control subjects had a positive occult blood disease. Conclusion:patients with hemophilia, H. pylori should not be considered as an important cause of GI bleeding. The recurrence of the infection and GI bleeding could be prevented with eradication of H. pylori. Screening tests for H. pylori would not be needed in patients with hemophilia in endemic areas.

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“…False‐negative results are caused by large doses of vitamin C 13. In addition, several of the chromogens commonly employed in conventional chemical assays (e.g., benzidine, o ‐tolidine, and diethyl‐ p ‐phenylenediamine) are carcinogenic and unstable during storage 18…”

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confidence: 99%

Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry for the detection of hemoglobins as the protein biomarkers for fecal occult blood

Lin

Shih

et al. 2007

Rapid Comm Mass Spectrometry

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In this study, we discover that hemoglobins (Hb), highly water-soluble globular proteins that are the most predominant proteins detected by matrix-assisted laser desorption ionization/time-of-flight (MALDI-TOF) mass spectrometry in blood, can be used as protein biomarkers for fecal occult blood (FOB). Hemoglobins were extracted from the feces with pure water and separated from the solids in feces through centrifugation. Singly charged molecular ions of Hb-related alpha chains (theoretical MW: 15 126) and beta chains (theoretical MW: 15 867) were detected by MALDI-TOF operated in linear mode using 4-hydroxy-alpha-cyanocinnamic acid (alpha-CHC) as the matrix (with a volumetric ratio of 1:1). The detection limit of FOB using this method is estimated to be lower than 0.1 microg blood per mg of feces, which is approximately 10 to 100 times lower than that of the conventional chemical approaches. The foods and dietary supplements that commonly interfere with the conventional chemical assays of FOB - such as animal blood food products and tablets containing iron and vitamin C - do not interfere with the detection of Hb biomarkers during MALDI-TOF analysis.

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New reagents for detection of faecal occult blood

Cited by 8 publications

References 12 publications

European guidelines for quality assurance in colorectal cancer screening and diagnosis. – First Edition Faecal occult blood testing

European guidelines for quality assurance in colorectal cancer screening and diagnosis. – First Edition Faecal occult blood testing

H Pylori Infection as Risk Factor for GIT Bleeding in Haemophilic Patients

Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry for the detection of hemoglobins as the protein biomarkers for fecal occult blood

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