New reagents for determination of amino acids by liquid chromatography with pre-column fluorescence derivatization

Fan, Xinjun; You, Jing; Kang, Jingwu; Ou, Qingyu; Zhu, Qingcun

doi:10.1016/s0003-2670(98)00125-1

Cited by 46 publications

(18 citation statements)

References 28 publications

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“…However, by varying the luminol and hexacianoferrate(III) concentrations in the range between 1.0 -10 mmol L -1 and 10 -100 mmol L -1 , respectively, no differences on the calculated limit of detection of H 2 O 2 was observed. In this way, considering that luminol is a expensive reagent, the chemiluminescent reagents concentrations was selected as 1 mmol L -1 luminol, and since better baseline was observed with 50 mmol L -1 K 3 [Fe(CN) 6 ], this solution concentration was selected as well. It interesting to report that since there was no blank signal, the standard deviation of 20 measurements of the baseline values (that is the baseline noise) was used to calculate the limit of detection.…”

Section: Resultsmentioning

confidence: 99%

“…4 For amino acid determination, ion exchange chromatography and more recently reverse phase high performance liquid chromatography have been extensile used for amino acid separation and determination 5 and since amino acids only absorb electromagnetic radiation between 220 and 254 nm, in order to improve detection, a derivation step has been implemented, either pre-column or post-column, for determination of low concentration by spectrophotometry or spectrofluorimetry methods. 6 An widely used derivatisation method is based on the reaction with ninhydrine producing a blue color compound. 7 This method provided high sensitivity, nevertheless it undergoes strong interference caused by ammonia and primaries amines resulting in high blank values.…”

Section: Introductionmentioning

confidence: 99%

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An enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine in synthesis process

Borges¹,

Reis²

2005

J. Braz. Chem. Soc.

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Neste trabalho é descrito um procedimento automático em fluxo, para a determinação de L-alanina em meio de síntese. O método é baseado na oxidação do aminoácido pela L-aminoacido oxidise, seguida da detecção do H 2 O 2 formado, pela reação de quimiluminescência com o luminol. Para condicionamento da amostra, como diluição e ajuste do pH, foi utilizada a estratégia da reamostragem. Pelo procedimento foi possível a determinação de L-alanina na faixa de 0,5 a 25,0 mmol L -1 , com um limite de detecção de 0,08 mmol L -1 e um coeficiente de variação menor que 4% (n = 10). Outras características vantajosas foram uma freqüência analítica de 44 determinações por hora e um baixo consumo de reagente: 0,2 mg de luminol e 16,4 mg de hexacinoferrato(III) por determinação.In this work an automatic flow procedure for L-alanine determination in the synthesis medium without a prior sample treatment is described. The method was based on oxidation of L-alanine by L-amino acid oxidize followed by the reaction of the H 2 O 2 with luminol and chemiluminescence detection. In order to overcome the severe alkaline condition of the synthesis medium, a zone sampling strategy was employed for automatic sample dilution and pH adjustment. The procedure allows L-alanine determination in the range of 0.5 to 25.0 mmol L -1 , a limit of detection of 0.08 mmol L -1 and a variation coefficient lower than 4% (n = 10). Other advantageous feature such as a sampling throughput of 44 determination per hour and low reagent consumption, 0.2 mg luminol and 16.4 mg hexacinoferrate(III) per determination, respectively, were also achieved.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine in synthesis process

Borges¹,

Reis²

2005

J. Braz. Chem. Soc.

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“…The raw material for synthesis of PIAA, 2-phenylindole, is non-toxic and inexpensive. Moreover, the relative fluorescence quantum efficiency of 2-phenylindole is much higher than that of carbazole [21], which has been used as a raw material for the synthesis of fluorescence reagents [22]. Without derivatization, the detection of DEG and its separation by reversed-phase HPLC would be difficult because DEG has no UV absorption or high polarity.…”

Section: Introductionmentioning

confidence: 99%

New derivatizing reagent for analysis of diethylene glycol by HPLC with fluorescence detection

Jie

Liu

et al. 2008

J of Separation Science

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N-(2-Phenyl-indolyl)-acetic acid (PIAA), a new fluorescent derivatizing reagent, was used for the determination of diethylene glycol (DEG) by high-performance liquid chromatography with fluorescence detection. DEG was derivatized to ester by using PIAA in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodimide hydrochloride (as dehydrating agent) and 4-(dimethylamino)pyridine (as base catalyst) in acetonitrile at 60 degrees C for 75 min. The influence of solvent, temperature, catalyst base, concentration of labeling reagent, and couple reagent on the derivatization was investigated. The fluorescence detection was performed with excitation at 340 nm and emission at 377 nm. Baseline separation was obtained on an Ultimate XB-C18 analytical column with water/acetonitrile gradient elution, good linearity was obtained within 0.5-50 microg/mL with a correlation coefficient of 0.9997. The limit of detection was 0.01 microg/mL (signal-to-noise ratio = 3). The method has been successfully applied to determine DEG in toothpaste samples with satisfactory recoveries ranging from 89.0 to 94.9%. The proposed method was shown to be a promising technique for the determination of DEG with high sensitivity.

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“…On the basis of the fluorescence characteristics of carbazole moiety [24], we synthesized a novel reagent, 2-(9-carbazole)-ethyl chloroformate (CEOC), which is easily accessible and very stable in its crystal state. In this study, with five monocyclic aromatic amines (o-toluidine, aniline, 3,4-dimethylaniline, N-ethyl-p-toluidine, p-phenylenediamine) as examples, effects of derivatization conditions, such as pH of buffer, reaction time and tagging reagent concentration, were investigated.…”

Section: Introductionmentioning

confidence: 99%

Analysis of aromatic amines by high-performance liquid chromatography with pre-column derivatization by 2-(9-carbazole)-ethyl-chloroformate

Zhang¹,

You²,

Ping³

et al. 2003

Analytica Chimica Acta

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New reagents for determination of amino acids by liquid chromatography with pre-column fluorescence derivatization

Cited by 46 publications

References 28 publications

An enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine in synthesis process

An enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine in synthesis process

New derivatizing reagent for analysis of diethylene glycol by HPLC with fluorescence detection

Analysis of aromatic amines by high-performance liquid chromatography with pre-column derivatization by 2-(9-carbazole)-ethyl-chloroformate

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