New Records of Four Species of Dactylogyrus (Monogenea: Dactylogyridae) from Goldfish, Carassius auratus (Linnaeus, 1758) (Pisces: Cyprinidae), in Cuba

The genus Dactylogyrus, comprising more than 900 taxa, is an important group of ectoparasites with high host specificity that causes disease and economic loss in ornamental fish. Dactylogyrus attaches to the host’s gills, causing respiratory disorders and potentially leading to fish mortality. This study was performed to develop a detection method for Dactylogyrus spp. infection in goldfish (Carassius auratus) using a loop-mediated isothermal amplification (LAMP) assay coupled with a lateral flow dipstick (LFD). The LAMP-LFD assay was performed in 75 minutes under isothermal conditions at 65°C. It demonstrated high specificity with no cross-amplification with related parasites or their definitive hosts. The sensitivity of the LAMP-LFD assay was 3 pg /µL for three Dactylogyrus species. Clinical sample examination showed that the accuracy of the LAMP-LFD assay was consistent with that of microscopy. In summary, the established LAMP-LFD assay targeting the 28S rDNA gene is a rapid and convenient method for detecting Dactylogyrus spp. in goldfish, aiding in parasite prevention, enhancing outbreak surveillance, and addressing veterinary health issues in the ornamental fish business.

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New Records of Four Species of Dactylogyrus (Monogenea: Dactylogyridae) from Goldfish, Carassius auratus (Linnaeus, 1758) (Pisces: Cyprinidae), in Cuba

Cited by 3 publications

References 7 publications

Seasonal dynamics of Dactylogyrus species (Monogenea: Dactylogyridae) on wild and farmed goldfish (Carassius auratus): Implication for prevention of dactylogyriasis

Seasonal dynamics of Dactylogyrus species (Monogenea: Dactylogyridae) on wild and farmed goldfish (Carassius auratus): Implication for prevention of dactylogyriasis

New records of nine monogenean parasites infesting non-native freshwater fish species in New Zealand

Development of a LAMP-LFD DNA biosensor for detecting the zoonotic monogenean Dactylogyrus spp. in goldfish (Carassius auratus)

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