New records of Pluteus section Hispidoderma in Turkey Based on Morphological Characteristics and Molecular Data

Abstract

Twenty-six species from various sections of the genus Pluteus have been recorded from Turkey until now. In this study, phylogenetic analyses inferred from the nuclear ribosomal internal transcribed spacer (nrITS) were conducted for species delimitation and for comparisons with closely related taxa of Pluteus sect. Hispidoderma in Turkey. Our results show that P. granularis is recorded in Turkey and Europe for the first time, P. granulatus in Turkey for the first time and in Europe for the third time, P. umbrosoides in Turkey for the first time and in the world for the second time, and P. variabilicolor in Turkey for the first time. Also in this study, sequencing data for P. leoninus, P. plautus, P. roseipes, P. semibulbosus and P. umbrosus as well as the above-mentioned new records are presented for the first time from Turkey as a different geographical area. Analyses of the ITS showed that the lowest sequence divergence among in-group taxa was observed between P. semibulbosus and P. aff. semibulbosus 0.010% (SE = 0.004), the next was between P. chrysaegis and P. conizatus var. africanus 0.012% (SE = 0.004). The highest sequence divergence with in-group taxa was found between P. plautus and P. umbrosoides, 0.351% (SE = 0.039). In terms of habitat, Fagus orientalis is recorded as a new host for P. granulatus, P. granularis, P. umbrosoides, and P. umbrosus; Picea orentalis is a new host to P. granularis; Carpinus betulus for P. umbrosus; and the relict endemic Liquidambar orientalis for P. variabilicolor. Finally, this study provides detailed morphological descriptions, macro photographs and microstructure drawings relating to species of Pluteus sect. Hispidoderma from Turkey, which are rare, interesting and noteworthy. An identification key is provided for present species of Pluteus sect. Hispidoderma occurring in the country.

Volvopluteus and Pluteus section Pluteus (Agaricales: Pluteaceae) in Turkey based on morphological and molecular data

“…Genomic DNA was extracted from fresh or dried materials using the ZR Fungal/Bacterial DNA MiniPrep kit (Zymo Research, Irvine, CA, USA). Protocols for DNA extraction, PCR and sequencing were the same as those outlined in Kaygusuz et al (2019Kaygusuz et al ( , 2020.…”

“…PCR amplification was carried out with ITS1F and ITS4 primers for the nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS) (White et al, 1990;Gardes and Bruns, 1993), LR0R and LR5 were used for the fragment of the nuc rDNA 28S (28S) (Vilgalys and Hester, 1990), and PNS1 and NS41 were used for the portion of the nuc rDNA 18S (18S) (Hibbett 1996). All primer pairs were used for amplification, using the touchdown PCR protocol described in Kaygusuz et al (2019Kaygusuz et al ( , 2020. The raw DNA sequencing files were assembled and edited using both Chromas Lite 2.1.1…”

“…Taxonomic and molecular studies carried out on Pluteus and Volvariella in Turkey (Kaya, 2000(Kaya, , 2010Sesli and Denchev, 2008;Solak et al, 2015;Kaygusuz et al, 2016;Sesli et al, 2016;Kaygusuz et al, 2019Kaygusuz et al, , 2020 have so far not included Pluteus sect. Pluteus and Volvopluteus from Turkey and hence, the current study was performed.…”

Two Novel Species and Two New Records within the Genus Pluteus (Agaricomycetes, Agaricales) from Thailand

“…Pluteus is a saprotrophic genus that can usually be found on the rotting logs of both angiosperms and gymnosperms [9]. It is distributed worldwide and has been widely documented in many continents such as Africa [10][11][12][13], Europe [5,9,, and North and South America [36][37][38][39][40][41][42][43][44].…”

“…Pluteus is a saprotrophic genus that can usually be found on the rotting logs of both angiosperms and gymnosperms [9]. It is distributed worldwide and has been widely documented in many continents such as Africa [10][11][12][13], Europe [5,9,, and North and South America [36][37][38][39][40][41][42][43][44]. However, two Pluteus species, P. bressollensis Eyssart., Ducousso & Buyck and P. fenzlii (Schulzer) Corriol & P. A. Moreau, are listed in the global IUCN Red List due to their limited population and the fact that their habitats have been adversely affected by climate change [45,46].…”