New Report of Distribution of Entomopathogenic Fungi Aschersonia sp. Infected Whitefly in Bali

Abstract

Laporan Baru tentang Distribusi Jamur Entomopatogenik Aschersonia sp. yang Menginfeksi Kutu Putih di Bali. Penelitian tentang distribusi jamur entomopatogenik Aschersonia sp. dilakukan untuk mengetahui persebaran Aschersonia yang menginfeksi kutu putih di seluruh Bali. Penelitian tersebut dilakukan di Kabupaten Gianyar, Bangli dan Buleleng pada centra perkebunan jeruk dimulai tahun 2014 sampai tahun 2018. Aschersonia pertama kali ditemukan pada tahun 2014 di Desa Sekaan Kintamani Bangli dan Desa Kerta Payangan Kabupaten Gianyar, dan pada tahun 2018 ditemukan di Desa Gobleg Sukasada Kabupaten Buleleng. Distribusi jamur tersebut ditemukan di dataran tinggi dan tidak ditemukan di dataran rendah. Hal ini sangat terkait dengan keadaan iklim terutama kelembaban dan suhu. Jamur Aschersonia sangat baik tumbuh pada suhu 20°C yang berada pada dataran tinggi.

First Report of <i>Cladosporium dominicanum Zalar</i>, de Hoog & Gunde-Cim. Infecting Whitefly on Ornamental Plants in Bali, Indonesia

“…First, DNA extraction was completed before the fungal DNA was amplified by PCR. Extraction of total DNA was conducted on a single colony of fungal growth on a PDA medium on a modified method performed using CTAB Doyle and Doyle method (Sudiarta et al 2019). Total DNA was used as a template for the Polymerase Chain Reaction (PCR).…”

“…Total DNA was used as a template for the Polymerase Chain Reaction (PCR). DNA amplification used primer set ITS-1 Forward (5'TCCGTAGGTGAACCTGCGG-3') and ITS-4 Reverse (5'-TCCCTCCGCTTATTGATATGC-3') targeting ± 560 bp of the ITS rDNA of Fungi (Sudiarta et al 2019). The 1 μl of PCR product (plus 2 μl of loading dye) was electrophoresed in 1% TBE agarose gel.…”

“…Aschersonia, Metarhizium, Beauveria, and Cladosporium are the common genera of fungi that infect whiteflies. The entomopathogenic fungi of the genus Aschersonia play a crucial role in controlling whitefly populations in subtropical and tropical regions worldwide (Sudiarta et al 2019;Bhosale et al 2020;Prayogo & Bayu 2020). In addition, the Aschersonia placenta was reported to infect the whitefly in citrus plants in Bali, Indonesia (Sudiarta et al 2019;Suputra et al 2019).…”

“…The entomopathogenic fungi of the genus Aschersonia play a crucial role in controlling whitefly populations in subtropical and tropical regions worldwide (Sudiarta et al 2019;Bhosale et al 2020;Prayogo & Bayu 2020). In addition, the Aschersonia placenta was reported to infect the whitefly in citrus plants in Bali, Indonesia (Sudiarta et al 2019;Suputra et al 2019). An in vitro study was carried out to assess the pathogenicity of an A. aleyrodis isolate against blackfly and whitefly (Bhosale et al 2020).…”

“…The identification was based on morphological and molecular characteristics using general DNA barcoding with primers ITS-1 and ITS-4. According to Sudiarta et al (2019) the DNA barcoding has been used successfully to confirm the species of entomopathogenic fungi.…”