New scaffolds encapsulating TGF-β3/BMP-7 combinations driving strong chondrogenic differentiation

Crecente‐Campo, José; Borrajo, Erea; Vidal, Anxo; García-Fuentes, Marcos

doi:10.1016/j.ejpb.2016.12.021

Cited by 45 publications

(31 citation statements)

References 40 publications

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“…Soluble factors individually and synergistically supported chondrocytes/stem cells toward chondrogenesis in both monolayer and pellet culture [91,99,200,208,209]. Supplementation with soluble factors, such as TGF-β, BMP and Mechano Growth Factor (MGF) IGF-I, into various scaffolds promoted efficient cartilage formation in human MSCs [210,211], indicating that these soluble factors could enhance chondrogenesis. Furthermore, it has been reported that TGF-β could bind to heparin sulfate [212] while GAGs stimulated the release of free and bioactive IGF-I in vitro [213], indicating that the interaction of soluble factors and GAGs may potentially influence chondrogenic differentiation.…”

Section: Chemical Componentsmentioning

confidence: 99%

Functionality of decellularized matrix in cartilage regeneration: A comparison of tissue versus cell sources

Sun

Yan²,

Chen

et al. 2018

Acta Biomaterialia

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The use of decellularized extracellular matrix (dECM) is becoming a promising approach for tissue engineering and regeneration. Compared to dECM derived from cartilage tissue, recently reported dECM from cell sources exhibits a distinct role in cell based cartilage regeneration. In this review paper, for the first time, tissue and cell based dECMs are comprehensively compared for their functionality in cartilage regeneration. This information is expected to provide an update for dECM based cartilage regeneration.

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Section: Chemical Componentsmentioning

confidence: 99%

Functionality of decellularized matrix in cartilage regeneration: A comparison of tissue versus cell sources

Sun

Yan²,

Chen

et al. 2018

Acta Biomaterialia

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“…Stimulating factors are necessary for cartilage engineering and responsible for inducing, accelerating, and/or enhancing cartilage formation. Common stimulating factors include BMP-2/-4, insulin-like growth factor (IGF)-1, and TGF-β1/-β3 [76,77,78]. Moreover, physical stimuli, such as hydrostatic pressure and dynamic compression, have been explored to induce MSC-mediated cartilage formation [79].…”

Section: Msc-based Regenerative Medicinementioning

confidence: 99%

Mesenchymal Stem Cells for Regenerative Medicine

Han

Zhang

et al. 2019

Cells

845

591

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In recent decades, the biomedical applications of mesenchymal stem cells (MSCs) have attracted increasing attention. MSCs are easily extracted from the bone marrow, fat, and synovium, and differentiate into various cell lineages according to the requirements of specific biomedical applications. As MSCs do not express significant histocompatibility complexes and immune stimulating molecules, they are not detected by immune surveillance and do not lead to graft rejection after transplantation. These properties make them competent biomedical candidates, especially in tissue engineering. We present a brief overview of MSC extraction methods and subsequent potential for differentiation, and a comprehensive overview of their preclinical and clinical applications in regenerative medicine, and discuss future challenges.

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“…133 PLGA scaffold with BMP-7/TGF-B3 nanocomplexesin vitro human MSCsControlled supplementation of BMP-7 can improve the chondrogenic effect of TGF-β3, and scaffolds loaded with this combination of growth factors can induce cartilage formation in hMSC culturesRef. 134 FGF-18 (Sprifermin)Intraarticular injectionHuman kneeqMRI showed increased cartilage thickness in a dose-dependent manner in knee OA patients with acceptable safety profile at 3 yearsRef. 135 Collagen membrane (Chondrogide)Sheep cartilage defectPotentiated the healing of a microfracture treated cartilage defect with improved weight bearing, O’Driscoll sore, and Type II collagen stainingRef.…”

Section: Advances In Cartilage Tissue Engineering and Regenerative Thmentioning

confidence: 99%

Emerging therapies for cartilage regeneration in currently excluded ‘red knee’ populations

et al. 2019

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The field of articular cartilage repair has made significant advances in recent decades; yet current therapies are generally not evaluated or tested, at the time of pivotal trial, in patients with a variety of common comorbidities. To that end, we systematically reviewed cartilage repair clinical trials to identify common exclusion criteria and reviewed the literature to identify emerging regenerative approaches that are poised to overcome these current exclusion criteria. The term "knee cartilage repair" was searched on clinicaltrials.gov. Of the 60 trials identified on initial search, 33 were further examined to extract exclusion criteria. Criteria excluded by more than half of the trials were identified in order to focus discussion on emerging regenerative strategies that might address these concerns. These criteria included age (<18 or >55 years old), small defects (<1 cm 2), large defects (>8 cm 2), multiple defect (>2 lesions), BMI >35, meniscectomy (>50%), bilateral knee pathology, ligamentous instability, arthritis, malalignment, prior repair, kissing lesions, neurologic disease of lower extremities, inflammation, infection, endocrine or metabolic disease, drug or alcohol abuse, pregnancy, and history of cancer. Finally, we describe emerging tissue engineering and regenerative approaches that might foster cartilage repair in these challenging environments. The identified criteria exclude a majority of the affected population from treatment, and thus greater focus must be placed on these emerging cartilage regeneration techniques to treat patients with the challenging "red knee".

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New scaffolds encapsulating TGF-β3/BMP-7 combinations driving strong chondrogenic differentiation

Abstract: Funding:Highlights:• The sustained delivery of BMP-7 enhances the chondrogenic activity of TGF-β3.• BMP-7 and TGF-β3 can be entrapped in polymers matrices as PEG-heparin complexes.• Scaffolds delivering TGF-β3 and BMP-7 drive the chondrogenesis of cell progenitors.

Cited by 45 publications

References 40 publications

Functionality of decellularized matrix in cartilage regeneration: A comparison of tissue versus cell sources

Functionality of decellularized matrix in cartilage regeneration: A comparison of tissue versus cell sources

Mesenchymal Stem Cells for Regenerative Medicine

Emerging therapies for cartilage regeneration in currently excluded ‘red knee’ populations

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