New Selective Medium for Isolation of Campylobacter Jejuni From Faecal Specimens

Butzler, Jean‐Paul; Boeck, M. De; Goossens, Herman

doi:10.1016/s0140-6736(83)91872-x

Cited by 18 publications

(11 citation statements)

References 2 publications

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“…Overall, these isolation rates were not different from those obtained by direct culture in this and previous studies [1,2]. GBS and CBA have been recommended for use as transport media for fecal specimens [3][4][5]. This pilot study demonstrates the long-term effectiveness of GBS for the isolation of common enteric pathogens.…”

Section: Discussionsupporting

confidence: 52%

“…2 g of stool sample were inoculated into each of two vials of transport media: GBS (Remel Media Laboratories, Lenexa, KA) and modified CBA (Remel). Cefoperazone, rifampin, colistin, and amphotericin B were the antibiotics added, as previously described [3]. Inoculated transport media were stored at room temperature until subculturing at the reference laboratory in Houston.…”

Section: Fecal Transport Systemmentioning

confidence: 99%

“…We designed a three-phase study to determine the feasibility of employing a fecal transport system to obtain reliable isolation of enteric pathogens from travelers throughout Latin America. We employed a two-vial system, GBS and modified CBA, to isolate bacterial pathogens [3][4][5]. First, we determined the length of time during which selected pathogens would remain viable.…”

Section: Introductionmentioning

confidence: 99%

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Isolation of enteric pathogens from patients with travelers' diarrhea using fecal transport media

Morgan¹,

Johnson²,

West³

et al. 1984

FEMS Microbiology Letters

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We investigated the effectiveness of a two‐vial fecal transport system: glycerol‐buffered saline (GBS) and modified Cary‐Blair with antibiotics (CBA) to transport stool samples to an enteric disease reference laboratory. In a blind study we compared the results of culturing 41 specimens promptly in the field laboratory to subculturing GBS and CBA in the reference laboratory. A pathogen was isolated from 72% of the cases in each laboratory. In addition, a pathogen was isolated from 32 of 45 samples inoculated into GBS and CBA and transported to the reference laboratory for subculture. These results demonstrate that GBS and CBA are effective means of transporting specimens to a reference laboratory when studying the etiology of diarrhea in remote parts of the world.

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Section: Discussionsupporting

confidence: 52%

Section: Fecal Transport Systemmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation of enteric pathogens from patients with travelers' diarrhea using fecal transport media

Morgan¹,

Johnson²,

West³

et al. 1984

FEMS Microbiology Letters

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“…In 1977, Skirrow [24] confirmed the findings of the Belgian team and described a simpler technique for culturing C. jejuni and C. coli from stool specimens, which allowed widespread isolation of these organisms. The later development of selective media, obviating the need to filter suspensions, brought the isolation of Campylobacter into the realm of routine microbiology [24][25][26][27][28][29][30]. Reports from industrialised countries, including European countries [19][20][21]24,[30][31][32][33], Canada [34] and the USA [35], and from developing countries, including Zaire [23] and Rwanda [36], have shown that Campylobacter enteritis occurs worldwide.…”

Section: The Breakthroughmentioning

confidence: 99%

“…jejuni and C. coli can be isolated easily from faeces by primary plating on selective media and incubation for 48-72 h at 42°C in a microaerobic atmosphere. A variety of selective media (bloodbased, blood-free charcoal-based) are available [24][25][26][27][28][29][30]. These contain antibiotics that suppress the normal bacterial enteric flora.…”

Section: I C R O B I O L O G Ymentioning

confidence: 99%

Campylobacter, from obscurity to celebrity

Butzler

2004

Clinical Microbiology and Infection

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396

296

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After its successful isolation from stools in the 1970s, Campylobacter jejuni has rapidly become the most commonly recognised cause of bacterial gastroenteritis in man. Reported cases of human campylobacteriosis represent only a small fraction of the actual number. In industrialised countries, the incidence of C. jejuni/Campylobacter coli infections peaks during infancy, and again in young adults aged 15-44 years. Acute self-limited gastrointestinal illness, characterised by diarrhoea, fever and abdominal cramps, is the most common presentation of C. jejuni/C. coli infection. The introduction of selective media has made the diagnosis of Campylobacter enteritis a simple procedure. In general, Campylobacter enteritis is a self-limiting disease which seldom requires antimicrobial therapy, although one in 1000 infections may lead to the Guillain-Barré syndrome. In industrialised countries, most infections are acquired through the handling and consumption of poultry meat. In developing countries, where the disease is confined to young children, inadequately treated water and contact with farm animals are the most important risk factors. Many infections are acquired during travel. Fluoroquinolone resistance has been reported in C. jejuni since the late 1980s in Europe and Asia, and since 1995 in the USA. The use of fluoroquinolones to treat animals used for food has accelerated this trend of resistance. In Australia, where fluoroquinolones have not been licensed for use in food production animals, C. jejuni remains susceptible to fluoroquinolones. The public health burden of Campylobacter spp. other than C. jejuni/C. coli remains unmeasured. Better diagnostic methods may reveal the true health burden of these organisms.

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