New SERS feature of β‐carotene: consequences for quantitative SERS analysis

Pinzaru, Simona Cîntă; Muller, Cornelius H.; Tomšić, Sanja; Venter, Monica M.; Cozar, Bogdan Ionuţ; Glamuzina, Branko

doi:10.1002/jrs.4713

Cited by 31 publications

(19 citation statements)

References 41 publications

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“…The profiles were placed manually and intuitively to serve as the initial parameters for curve fitting. The deconvoluted spectra contain variations in peak positions, which were then calibrated using the peak position of the pure β-carotene 43 as a reference marker.…”

Section: Methodsmentioning

confidence: 99%

Classification of oil palm fresh fruit maturity based on carotene content from Raman spectra

Raj

Hashim

Huddin

et al. 2021

Sci Rep

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The oil yield, measured in oil extraction rate per hectare in the palm oil industry, is directly affected by the ripening levels of the oil palm fresh fruit bunches at the point of harvesting. A rapid, non-invasive and reliable method in assessing the maturity level of oil palm harvests will enable harvesting at an optimum time to increase oil yield. This study shows the potential of using Raman spectroscopy to assess the ripeness level of oil palm fruitlets. By characterizing the carotene components as useful ripeness features, an automated ripeness classification model has been created using machine learning. A total of 46 oil palm fruit spectra consisting of 3 ripeness categories; under ripe, ripe, and over ripe, were analyzed in this work. The extracted features were tested with 19 classification techniques to classify the oil palm fruits into the three ripeness categories. The Raman peak averaging at 1515 cm−1 is shown to be a significant molecular fingerprint for carotene levels, which can serve as a ripeness indicator in oil palm fruits. Further signal analysis on the Raman peak reveals 4 significant sub bands found to be lycopene (ν1a), β-carotene (ν1b), lutein (ν1c) and neoxanthin (ν1d) which originate from the C=C stretching vibration of carotenoid molecules found in the peel of the oil palm fruit. The fine KNN classifier is found to provide the highest overall accuracy of 100%. The classifier employs 6 features: peak intensities of bands ν1a to ν1d and peak positions of bands ν1c and ν1d as predictors. In conclusion, the Raman spectroscopy method has the potential to provide an accurate and effective way in determining the ripeness of oil palm fresh fruits.

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Section: Methodsmentioning

confidence: 99%

Classification of oil palm fresh fruit maturity based on carotene content from Raman spectra

Raj

Hashim

Huddin

et al. 2021

Sci Rep

View full text Add to dashboard Cite

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“…The peak at 1,006 cm –1 occurs due to methyl in‐plane rocking modes. The high‐intensity peaks at 1,157 and 1,520 cm –1 were attributed to the in‐phase symmetric stretching of C–C and C=C of the carotenoid polyene chain, respectively (Cintă Pinzaru et al., 2015; Huo et al., 2011; Killeen et al., 2013).…”

Section: Resultsmentioning

confidence: 99%

Study of three drying methods in production of nutritious flours from the fermentation slurry of orange‐fleshed sweet potato

Schweinberger

Sebben

Schultz

et al. 2020

J Food Process Preserv

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When producing ethanol from sweet potatoes, the fermentation generates slurry with nutritional content but underutilized for human consumption. Therefore, the nutritional properties of the flours obtained from the fermentation slurry of orange‐fleshed sweet potato (rich in carotenoids) was investigated. Three drying methods were carried out to produce slurry flours: convective using a hot‐air (60°C), microwave, and lyophilization. For carotenoids determination, were applied a conventional method and the Raman spectroscopy, a modern and alternative technique. Regarding the drying methods, the microwave was the most promising since it produced flour presenting higher levels of carotenoids (β‐carotene 849.7 µg/g and lycopene 548.4 µg/g). Also, the microwave drying time was 15 min, being much faster than the other methods. In the centesimal analysis of macronutrients, the slurry flours presented high fiber content, around 55 g/100 g. Finally, biscuits containing parcels of the flours demonstrated potential acceptability, by sensory analysis evaluating aroma and appearance. Practical applications Since ethanol is a product of worldwide importance, and it is produced mainly from corn and sugar cane, the production from sweet potatoes as an alternative raw material has interested researchers from different countries. Knowing that the ethanol production from sweet potato generates a residual slurry, this study focuses on adding value to this slurry, producing a nutritious flour. The potential for industrial application is promising, assuming it is a residue of an industrial infrastructure already installed. For the industry, obtaining more than one product in the same process represents a financial advantage and resource optimization.

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“…Multiple carotenoid species may contribute to the band profile. The v2 band at 1157 cm -1 arises from single C-C bonds in the polyene chain, while the v3 band, which is mainly caused by methyl rocking, was clearly split into two peaks at 1000 and 1013 cm -1 (Tschirner et al, 2009;Cintă Pinzaru et al, 2015).…”

Section: Surface-enhanced Raman Scatteringmentioning

confidence: 98%

Comparative Raman Spectroscopy Study of the Coelomic Fluid of Grazing Sea Urchins and Their Native Seawater: Prospect for A Potential Indicator of Environmental Aggression

Nekvapil¹,

Tomšić²,

Pinzaru³

2018

Air and Water Components of the Environment Conference

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Sea urchins have limited ability to move, and they use the watervascular circulation system to pump surrounding sea water in order to set body parts in motion. It is therefore thought that coelomic fluid, a body fluid of sea urchins which acts as its internal transport and immune system, contains metabolised pollutants. In the present study, we developed a method for detection of carotenoids in the coelomic fluid by Raman spectroscopy. Carotenoids were obtained from the coelomic fluid by ethanol extraction and their selective resonance Raman signal was enhanced employing the surface enhanced Raman scattering (SERS) technique. Carotenoids are ingested by sea urchins through their plant-based diet, metabolised and transported into the coelomic fluid, where they were detected for the first time via SERS. We further investigated the correlations of carotenoids signalling from the coelomic fluid with the local sea water to prospect a potential linkage with changes under environmental aggression. The antioxidative and immunomodulatory role of carotenoids, especially of β-carotene, was extensively studied in vertebrates (Chew and Park, 2004). Biological defence and increased antioxidant activity is associated with an increased carotenoids level and/or change in their species balance in native sea urchins. Additionally, we compared relative sulfate concentration of sea urchin coelomic fluid with local sea water using FT-Raman technique. We discuss the possibility for development of methods for rapid and cost effective monitoring of the native environmental waters via sea urchin carotenoids. Other co-existent pollutants which may enter the coelomic fluid via the digestive system or through water-vascular system of sea urchins are expected to correlate with the animal response via an increased antioxidant activity due to carotenoids. Thus, sea urchins may prove to be good sentinels of environmental water changes via their carotenoids signalling.

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New SERS feature of β‐carotene: consequences for quantitative SERS analysis

Cited by 31 publications

References 41 publications

Classification of oil palm fresh fruit maturity based on carotene content from Raman spectra

Classification of oil palm fresh fruit maturity based on carotene content from Raman spectra

Study of three drying methods in production of nutritious flours from the fermentation slurry of orange‐fleshed sweet potato

Comparative Raman Spectroscopy Study of the Coelomic Fluid of Grazing Sea Urchins and Their Native Seawater: Prospect for A Potential Indicator of Environmental Aggression

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