New set of microsatellite markers for the Great-billed Seed-finch (Sporophila maximiliani – Passeriformes: Thraupidae): tools for inspection and conservation

Melo, Amanda Alves de; Kalapothakis, Evanguedes; Ludwig, Sandra; Baptista, Luiz A. M. L.; Lara, Mariana P.; Silva, Leo C. F.; Telles, Mariana Pires de Campos

doi:10.1007/s11033-020-05320-x

Cited by 2 publications

(1 citation statement)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, microsatellites have played an increasingly important role in the wildlife conservation ( Moura et al, 2017 ), paternity testing ( Correia et al, 2021 ), and meat source traceability ( Kannur et al, 2017 ) in recent years. Due to the close geographical location, some chicken breeds are difficult to distinguish by shape and are rare breeds, therefore to protect the needs of breeds, molecular tags are needed for more accurate breed identification ( de Melo et al, 2020 ). This study not only helps to understand the origin of domestic chickens and genetic differences of these breeds in this region but also assists in the evaluation of breeding potential and provides a basis for realizing the construction of new germplasm under the premise of meeting the protection of germplasm resources.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and breed identification of Chinese and Vietnamese local chicken breeds based on microsatellite analysis

Zhuang

Zhao

Zong

et al. 2023

Journal of Animal Science

View full text Add to dashboard Cite

South Asia and Southeast Asia are the origins of domestic chickens and are rich in poultry genetic resources, resulting in many unique local chicken breeds. However, with the rapid intensification of poultry farming worldwide, many local chicken breeds are threatened with extinction. In response to China's "One Belt, One Road" policy, it is imperative to strengthen the conservation and breeding of local chicken breeds in China and Vietnam. This study characterized 18 microsatellite molecular genetic markers to analyze the genetic diversity of 21 local chicken populations in southern China (Yunnan and Guangxi Provinces) and Vietnam, Breed identification tags for microsatellite loci were constructed. The results showed that a total of 377 alleles were detected in all breeds, and the most alleles (44) and highest polymorphic information content (0.7820) were detected at the LEI0094 locus. The average polymorphic information content (PIC) content of the whole population was 0.65, indicating moderate polymorphism. The genetic diversity of the whole population was rich, except for two loci MCW0111 and MCW0016, that showed heterozygote excess at microsatellite loci, and the population had high genetic differentiation. The Vietnamese breeds showed low pairwise fixation coefficient (FST) and Nei's standard genetic distance (DS) between them. According to the neighbor-joining dendrogram constructed by DS and the analysis of population genetic structure using the Structure program, Longshengfeng chicken, Yunlong dwarf chicken, Tengchong white chicken, Xiayan chicken, and Daweishan mini chicken are similar, and Xishuangbanna game fowl, Wuding chicken, and Lanping silky chicken are similar to Yanjin black-bone chicken. In addition, excluding Dongtao chicken, other Vietnamese breeds are clustered together, indicating that the southern chicken breeds are closely related and have experienced better breeding. Overall, the whole population is rich in genetic resources, and the chicken breeds in the three regions are genetically close because of geographical factors and human activities. Dongtao chicken in Vietnamese, Chinese Yunnan local chicken breeds (Gallus gallus spadiceus), and red jungle fowl chickens (Gallus gallus) may have the same origin. We also constructed unique microsatellite molecular markers for 20 cultivars using 15 microsatellite loci. This study provides valuable insights to facilitate breed identification, and improve cultivar protection, and new germplasm construction.

show abstract

Section: Introductionmentioning

confidence: 99%

Genetic diversity and breed identification of Chinese and Vietnamese local chicken breeds based on microsatellite analysis

Zhuang

Zhao

Zong

et al. 2023

Journal of Animal Science

View full text Add to dashboard Cite

show abstract

Analysis of the Relationship between Short Tandem Repeats and Lactation Performance of Xinjiang Holstein Cows

LIU

ABULA

et al. 2023

Preprint

View full text Add to dashboard Cite

Microsatellite markers, also known as short tandem repeats (STRs), are important for marker-assisted selection to detect genetic polymorphism, and they are uniformly distributed in eukaryotic genomes. To analyze the relationship between microsatellite loci and lactation traits of Holstein cows in Xinjiang, 175 lactating cows with similar birth dates, the same parity, and similar calving dates were selected, and 10 STR loci closely linked to quantitative trait loci were used to analyze the correlation between each STR locus and 4 lactation traits (daily milk yield, milk fat percentage, milk protein percentage, and lactose percentage). All loci showed different degrees of genetic polymorphism. The average values of observed alleles, effective alleles, expected heterozygosity, observed heterozygosity, and polymorphic information content of the 10 STR loci were 10, 3.11, 0.62, 0.64, and 0.58, respectively. Chi-square and G-square tests showed that all populations of loci were in accordance with the Hardy–Weinberg equilibrium. Analysis of the correlation between STR locus genotype and lactation performance in the whole lactation period showed 3 loci (namely, BM143, BM415, and BP7) with no significant correlation with all lactation traits, 2 loci (BM302 and UWCA9) related to milk yield, 3 loci (BM103, BM302, and BM6425) related to milk fat percentage, 2 loci (BM302 and BM6425) related to milk protein percentage, and 3 loci (BM1443, BM302, and BMS1943) related to lactose percentage. The microsatellite loci selected in this study showed rich polymorphism in the experimental dairy cow population and were related to the lactation traits, which can be used for the evaluation of genetic resources and early breeding and improvement of Holstein dairy cows in Xinjiang.

show abstract

New set of microsatellite markers for the Great-billed Seed-finch (Sporophila maximiliani – Passeriformes: Thraupidae): tools for inspection and conservation

Cited by 2 publications

References 16 publications

Genetic diversity and breed identification of Chinese and Vietnamese local chicken breeds based on microsatellite analysis

Genetic diversity and breed identification of Chinese and Vietnamese local chicken breeds based on microsatellite analysis

Analysis of the Relationship between Short Tandem Repeats and Lactation Performance of Xinjiang Holstein Cows

Contact Info

Product

Resources

About