New Solvent System for Separation of Amino Acids by Paper Chromatography

Clayton, Russell; Strong, F. M.

doi:10.1021/ac60092a030

Cited by 50 publications

(16 citation statements)

References 4 publications

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“…Chromatographic determination of amino acids. The methods of Clayton & Strong (1954) and of Meyer (1957) were used. After incubation, the tissue suspension was added to an equal volume of boiling ethanol.…”

Section: Methodsmentioning

confidence: 99%

The interconversion of glutamic acid and aspartic acid in respiring tissues

Krebs

Bellamy

1960

Biochemical Journal

147

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of glyoxylate, or oxalate, in the presence of acetylcoenzyme A. 8. It is suggested that during growth on oxalate, the oxalate, possibly in the form ofoxalyl-coenzyme A, is reduced to glyoxylate, which is taken to be the precursor of glycine and phosphoglycerate. The phosphoglycerate formed then enters established routes of biosynthesis. We wish to thank Professor Sir Hans A. Krebs, F.R.S., for his interest and encouragement and Mrs M. Jenkins for her skilled technical assistance. This work was supported by the Rockefeller Foundation and by the Office of Scientific Research of the Air Research and Development Command of the United States Air Force, through its European Office, under contract no. AF. 61(052)-180, and was done while one of us (D. B. K.) held an Overseas Scholarship of the Commonwealth Scientific and Industrial Research Organization (Australia).

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Section: Methodsmentioning

confidence: 99%

The interconversion of glutamic acid and aspartic acid in respiring tissues

Krebs

Bellamy

1960

Biochemical Journal

147

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“…A 50-vl. sample of each urinc collection was subjected to paper chromatography by either the two-dimensional system of Datta, Dent and Harris (1950) or a one-dimensional system (Clayton and Strong, 1954). Location ofthe amino-acids on the paper after chromatographic separation was achieved by spraying the dried paper with 0.5 per cent ninhydrin in butanol.…”

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confidence: 99%

Amino‐Acid and Protein Metabolism in Pernicious Anaemia

Neill¹,

Weaver²

1958

Br J Haematol

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“…Amino acids were tested with ascending and circular chromatography, using the following solvents: (i) 1butanol-acetic acid-water (40:10:50; v/v; Saifer and Oreskes, 1956); (ii) water-saturated phenol (Saifer and Oreskes, 1956); and (iii) methyl ethyl ketone-propionic acid-water (75:25:30;v/v;Clayton and Strong, 1954).…”

Section: Methodsmentioning

confidence: 99%

Induced Autolysis of Aspergillus oryzae (A. niger group)

Emiliani¹,

Davie²

1962

Applied Microbiology

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AND I. UCHA DE DAVIE. Induced autolysis of Aspergillus phoenicis (A. niger group). IV. Carbohydrates. Appl. Microbiol. 10:504-512. 1962.-The examination of substances formed during induced autolysis by Aspergillus niger was continued in this work, which dealt in particular with carbohydrates. The autolysate contained a large amount of D-glucose (14 to 20 % dry wt) and traces of glycolic aldehyde, dihydroxyacetone, ribose, xylose, and fructose. It also contained glycopeptides (about 10 % dry wt), which were split from the cell wall during autolysis and which differed from one another in their level of polymerization and their composition. They were constituted by glucose and mannose, glucose and galactose, or mannose, glucose, and galactose (mannose being the most abundant in this case), and amino acids (chiefly alanine, serine, glutamic acid, and aspartic acid). During autolysis, only a part of the cell wall was dissolved, since it retained its shape. Upon further chemical hydrolysis, it produced mostly glucose and glucosamine, and smaller amounts of mannose, galactose, and amino acids. Presumably, glucomannoproteins and glucogalactoproteins were present in the intact cell as a macromolecular complex, constituting, together with chitin, the major part of the cell wall of Aspergillus.

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New Solvent System for Separation of Amino Acids by Paper Chromatography

Cited by 50 publications

References 4 publications

The interconversion of glutamic acid and aspartic acid in respiring tissues

The interconversion of glutamic acid and aspartic acid in respiring tissues

Amino‐Acid and Protein Metabolism in Pernicious Anaemia

Induced Autolysis of Aspergillus oryzae (A. niger group)

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