New sources of resistance to PepMV in tomato

Soler, Salvador; López, Carmelo; Prohens, Jaime; Nuez, F.

doi:10.1007/bf03356397

Cited by 5 publications

(9 citation statements)

References 16 publications

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“…Although several attempts were underway to find sources of genetic resistance to PepMV within accessions of tomato and wild relatives, to the best of our knowledge, no PepMV-resistant tomato varieties are yet available [102,103]. In addition, PepMV comprises at least four different genetic groups; different isolates cause different symptoms depending on hosts and these might harbor strains that can overcome potential resistance [105,109,110,112,113,114,115,116,117,118,119,120,121,122]. The observation of naturally occurring mild PepMV strains with little or no impact on fruit symptoms and/or yield led to the proposal to use those mild strains for cross-protection purposes [105,123,124,125].…”

Section: Commercialization Of Mild Strain Cross-protection: Case Smentioning

confidence: 99%

A New Era for Mild Strain Cross-Protection

Pechinger

Chooi

MacDiarmid

et al. 2019

Viruses

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Societal and environmental pressures demand high-quality and resilient cropping plants and plant-based foods grown with the use of low or no synthetic chemical inputs. Mild strain cross-protection (MSCP), the pre-immunization of a plant using a mild strain of a virus to protect against subsequent infection by a severe strain of the virus, fits with future-proofing of production systems. New examples of MSCP use have occurred recently. New technologies are converging to support the discovery and mechanism(s) of action of MSCP strains thereby accelerating the popularity of their use.

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Section: Commercialization Of Mild Strain Cross-protection: Case Smentioning

confidence: 99%

A New Era for Mild Strain Cross-Protection

Pechinger

Chooi

MacDiarmid

et al. 2019

Viruses

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“…Additionally, given that cucumber does not always display symptoms, the use of clonal replicas or the use of progeny tests may be needed for screening individual genotypes of segregating populations in breeding programmes. However, symptomatology alone may not be appropriate to evaluate infection by ToLCNDV in cucurbit plants in commercial or experimental fields, as symptoms may be caused by other diseases or the virus may not be randomly distributed (Soler et al ., ).…”

Section: Resultsmentioning

confidence: 97%

Inoculation of cucumber, melon and zucchini varieties with Tomato leaf curl New Delhi virus and evaluation of infection using different detection methods

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Alfaro-Fernández

Font

et al. 2017

Annals of Applied Biology

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The disease caused by Tomato leaf curl New Delhi virus (ToLCNDV), which is naturally transmitted by the whitefly Bemisia tabaci, causes important economic losses in cucurbit crops. The availability of simple and efficient inoculation protocols and detection methods is necessary for screening varieties and germplasm collections as well as for breeding populations. We evaluated the infectivity of ToLCNDV inocula prepared using three different buffers for mechanical sap inoculation in a susceptible variety of zucchini. We found that inoculum prepared with buffer III, which contains polyvinylpyrrolidone, is highly efficient for mechanical inoculation, with 100% of plants displaying severe symptoms 21 days post-inoculation. Using this buffer, we mechanically inoculated 19 commercial varieties of cucurbit crops (six of cucumber, six of melon and seven of zucchini), evaluated the evolution of symptoms and diagnosed infection using nine different ToLCNDV detection methods (four based on serology, four based on molecular hybridization and one based on PCR detection). The results revealed that all varieties are susceptible, although cucumber varieties display less severe symptoms than those of melon or zucchini. All detection methods were highly efficient (more than 85% of plants testing positive) in melon and zucchini, but in cucumber, the percentage of positive plants detected with serology and molecular hybridization methods ranged from 20.4% with Squash leaf curl virus (SLCV) antiserum, to 78.5% with DNA extract hybridization. Overall, the best detection results were obtained with PCR, with 92.6%, 92.4% and 98.4% cucumber, melon and zucchini plants, respectively, testing positive. When considering the overall results in the three crops, the best serology and molecular hybridization methods were those using Watermelon chlorotic stunt virus (WmCSV) antiserum and DNA extract, respectively. The inoculation methodology developed and the information on detection methods are of great relevance for the selection and breeding of varieties of cucurbit crops that are tolerant or resistant to ToLCNDV.

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“…The fact that viable somatic hybrids between tomato and pepino have been obtained (Sakomoto & Taguchi, 1991), may represent a way to transfer the resistance from S. caripense accession Car1 to tomato. However, as in tomato, some cultivated accessions and wild materials have shown different degrees of tolerance to PepMV (Soler et al, 2011). In the case of ToMV, no resistance has been found in the evaluated cultivated and wild materials selected for this study, so we suggest resorting to other materials that have previously been identified as resistant (Leiva‐Brondo et al, 2006), and which in fact have already been used to develop a ToMV resistant cultivar (Rodríguez‐Burruezo, Prohens, Leiva‐Brondo, & Nuez, 2004b).…”

Section: Discussionmentioning

confidence: 99%

“…PepMV, a potexvirus that was first isolated from infected pepino plants in 1980 (Jones et al, 1980), causes important losses worldwide in tomato production, especially in Europe and North America (Souiri et al, 2017). The symptoms in pepino include yellow mosaic in young leaves (Jones et al, 1980), while in tomato are very diverse, and may occur in the form of fruit discoloration, chlorosis and yellow angular leaf spots, severe leaf mosaics and occasionally leaf or stem necrosis (Hanssen & Thomma, 2010; Hasiów‐Jaroszewska & Komorowska, 2013; Sempere et al, 2016; Soler, López, Prohens, & Nuez, 2011). PepMV is transmitted mechanically with high efficiency, mainly during cultural pruning and fruit harvesting practices through contaminated tools and clothing (Hasiów‐Jaroszewska, Borodynko, Jackowiak, Figlerowicz, & Pospieszny, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Resistant rootstocks are also commonly used in tomato for resistance to FOL and VE (King, Davis, Zhang, & Crosby, 2010). However, so far, no effective resistance against PepMV has been incorporated in tomato (Pechinger, Chooi, MacDiarmid, Ziebell, & Harper, 2019), although some sources of resistance have been identified in the wild tomato S. lycopersicoides (Soler et al, 2011) and in tomato accessions 11R.412000 and 11R.446400 (US patent US9637757B2). In pepino, several accessions resistant to ToMV have been described, although its genetic control has not been determined so far (Leiva‐Brondo et al, 2006; Pérez‐Benlloch et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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