New specific assays for tonin and tissue kallikrein activities in rat submandibular glands

Shori, Deepak K.; Proctor, Gordon; Chao, Julie; Chan, Ka-Ming; Garrett, J. R.

doi:10.1016/0006-2952(92)90494-4

Cited by 32 publications

(11 citation statements)

References 27 publications

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“…Tissue kallikrein activity was assayed by means of the peptide substrate DVLR‐AFC ( d ‐valine‐leucine‐arginine‐7‐amino‐4‐trifluoromethylcoumarin) as previously described by Shori et al . (1992).…”

Section: Methodsmentioning

confidence: 99%

Intraoral duct ligation without inclusion of the parasympathetic nerve supply induces rat submandibular gland atrophy

Osailan

Proctor

McGurk

et al. 2006

Int J Experimental Path

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The atrophic effect of ligating the main duct of the right submandibular gland was examined in rat using a novel intraoral approach that did not include the chorda lingual (CL) nerve. Comparison was made with the effect of duct ligation including the attached CL nerve as carried out in previous studies. In all animals, the contralateral, unligated left submandibular gland was used as a control. At different times (1, 2, 7, 14 and 21 days) after ligation, glands were removed and weighed. Tissue was fixed for morphological analysis and homogenized for biochemical assay of secretory proteins. After 21 days, ligated glands showed a significant decrease in wet weight compared with unligated glands. Weight loss was the greatest (P < 0.05) in glands ligated with the CL nerve included. Light microscopy revealed that following ligation, an initial inflammatory reaction was followed by severe atrophy of acini and granular ducts. The atrophy was less severe when the CL nerve was not ligated. Secretory proteins were decreased from day 1 onwards following duct ligation in both groups. It can be concluded that most of the atrophy induced by duct ligation is independent of damage caused to the parasympathetic nerve supply, although the latter causes a greater atrophy presumably due to denervation.

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Section: Methodsmentioning

confidence: 99%

Intraoral duct ligation without inclusion of the parasympathetic nerve supply induces rat submandibular gland atrophy

Osailan

Proctor

McGurk

et al. 2006

Int J Experimental Path

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“…From the kallikrein superfamily, tissue kallikrein is the major proteinase that shows relative resistance to inhibition by SBTI. 21 Our results showed that the introduction of the human kallikrein gene into rat genome produced alterations in the activity and gene expression of the enzymes. For kallikrein, in general, we observed augmented activity and mRNA levels.…”

Section: Discussionmentioning

confidence: 66%

Tonin and Kallikrein in the Brain of Transgenic Rat Line Expressing Human Tissue Kallikrein

et al. 2002

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Abstract-A transgenic rat line harboring the human tissue kallikrein gene was investigated for expression and activity of tonin and kallikrein in different regions of the brain. The introduction of the transgene into the rat genome produced a significant augmentation of the expression levels and activity of rat tissue kallikrein. The possibility that human kallikrein does not hydrolyze the rat substrate is probably responsible for the augmented expression of the rat enzyme.On the other hand, although expression of tonin was significantly reduced, tonin activity was not altered in most brain structures, except for cerebellum and neurohypophysis. (Hypertension. 2002;39:229-232.)

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“…The kallikreins, originating from granular tubule cells, are present in the major bands between 25-35 KD, as indicated by enzymic activities (Figure 4) and by antibodies on Western blots (Shori et al, 1992a). These glycoproteins were found by appropriate lectins to contain abundant aFuc, NeuAc, and terminal aGalNAc, along with small amounts of terminal BGal, both in glandular extracts and in saliva.…”

Section: Discussionmentioning

confidence: 92%

“…developing in 3% sodium carbonate with 0.05% formaldehyde) as described by Jay et al (190). The proteolytic activity of secretory kallikreins was demonstrated as previously described (Shori et al, 1992a). Briefly, SDS was washed out of gels by soaking in two changes of 0.1 M Trk-HC1 buffer, pH 8.0.…”

Section: Methodsmentioning

confidence: 99%

Use of lectin probes on tissues and sympathetic saliva to study the glycoproteins secreted by rat submandibular glands.

Zhang¹,

Proctor²,

Garrett³

et al. 1994

J Histochem Cytochem.

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We used a panel of nine lectins to detect the glycosylation patterns of rat submandibular glycoproteins. Binding of lectins was assessed on tissue sections and on Western blots of electrophoretically separated glycoproteins from glandular extracts or sympathetic saliva. Histochemical staining of tissue sections showed that two lectins (DBA and SBA) with affinity for terminal GalNAc residues were localized specifically to acinar cells. In contrast, LTA and UEA-I (alpha Fuc-directed) and LFA (NeuAc-directed) bound exclusively to granular tubule cells. PNA and MPA (beta Gal-directed), LCA (alpha Man- and alpha Glc-directed), WGA (beta GlcNAc- and NeuAc-directed), and sWGA (beta GlcNAc-directed) bound to both acinar and granular tubule cells. On electroblot preparations, LFA, PNA, WGA, DBA, and SBA reacted with high molecular weight acinar mucin components both in glandular extracts and in saliva. LTA, UEA-I, LFA, PNA, MPA, LCA, WGA, sWGA, and DBA bound to lower molecular weight bands on blots known to contain granular tubular proteinases. Lectin binding to acini and granular tubules was reduced in sections and in most bands from glandular extracts after sympathetic nerve stimulation. Our results show that (a) secretory glycoproteins from rat submandibular acinar cells are non-fucosylated and contain abundant alpha GalNAc and NeuAc and a small proportion of beta Gal in their oligosaccharide side chains, and (b) alpha Fuc, NeuAc, beta Gal, and alpha GalNAc form the major carbohydrate moieties of the secretory glycoproteins from granular tubules. The results confirm the considerable potential of lectin probes for studying glycoproteins in secretions and in their cells of origin.

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New specific assays for tonin and tissue kallikrein activities in rat submandibular glands

Cited by 32 publications

References 27 publications

Intraoral duct ligation without inclusion of the parasympathetic nerve supply induces rat submandibular gland atrophy

Intraoral duct ligation without inclusion of the parasympathetic nerve supply induces rat submandibular gland atrophy

Tonin and Kallikrein in the Brain of Transgenic Rat Line Expressing Human Tissue Kallikrein

Use of lectin probes on tissues and sympathetic saliva to study the glycoproteins secreted by rat submandibular glands.

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