New Specific α‐Glucosidase Inhibitor Flavonoid from <i>Thymelaea tartonraira</i> Leaves: Structure Elucidation, Biological and Molecular Docking Studies

Soltani, Siwar; Koubaa, Imed; Dhouib, Ines Bini; Khemakhem, Bassem; Marchand, Pascal; Allouche, Noureddine

doi:10.1002/cbdv.202200944

Cited by 12 publications

(4 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The tested extracts demonstrated notable antioxidant potency through various mechanisms. Specifically, the methanol extract exhibited effectiveness in reducing Fe 3+ to Fe 2+ , converting Mo 6+ to Mo 5+ , and scavenging free radicals, which are known to pose significant health risks to humans [44]. The observed antioxidant activity of the methanolic extract from Cynoglossum tubiflorus leaves can be attributed to its abundant content of phenolic compounds and flavonoids, as suggested by previous studies [42].…”

Section: Total Antioxidant Activity (Tac)mentioning

confidence: 53%

See 1 more Smart Citation

HPLC-HESI-MS/MS Analysis of Phenolic Compounds from Cynoglossum tubiflorus Leaf Extracts: An Assessment of Their Cytotoxic, Antioxidant, and Antibacterial Properties

Dallali,

Fakhfakh,

Paris

et al. 2024

Plants

Self Cite

View full text Add to dashboard Cite

The current study aimed to investigate the chemical composition, antioxidant, antibacterial, and cytotoxic properties of three extracts (hexane, dichloromethane, and methanol) from Cynoglossum tubiflorus. The composition of the methanolic extract was elucidated using HPLC-HESI-MS/MS analysis. The antioxidant effect was examined using NO, DPPH, FRAP, and TAC assays. Antimicrobial activity was evaluated by broth microdilution using various bacterial strains such as S. aureus, S. epidermidis, P. aeruginosa, E. coli, and K. pneumoniae. Structural disruptions in Gram-positive bacteria were visualized using scanning electron microscopy (SEM). Cytotoxic effects were evaluated on human MRC-5 in culture according to the MTT assay. The outcomes suggest that methanol extract contained a high amount of phenolic compounds (254.35 ± 0.360 mg GAE/g DE and 211.59 ± 0.939 mg QE/g DE). By applying the HPLC-HESI-MS/MS analysis, 32 compounds were identified, including phenolic acids, flavonoids, lignans, and fatty acids. This extract showed strong antioxidant (IC50 = 0.043 ± 0.001 mg/mL) and antimicrobial (MIC = 156 µg/mL) activities. The SEM suggests that cells exhibited membrane distortions characterized by surface depressions and alterations in bacterial shape, including dents, when compared to untreated cells. The in vitro cytotoxicity effect on human MRC-5 cells showed no toxicity effects at a concentration of 600 µg/mL. In silico analysis predicted low toxicity for all tested compounds across four different administration routes. This research indicates that this plant could be explored as a powerful source of natural drugs to target pathogens, with applications in the food, pharmaceutical, and medical industries.

show abstract

Section: Total Antioxidant Activity (Tac)mentioning

confidence: 53%

Section: Resultsmentioning

confidence: 99%

HPLC-HESI-MS/MS Analysis of Phenolic Compounds from Cynoglossum tubiflorus Leaf Extracts: An Assessment of Their Cytotoxic, Antioxidant, and Antibacterial Properties

Dallali,

Fakhfakh,

Paris

et al. 2024

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…Ertas et al (2016) determined that the flavonoid content (19.66 ± 1.58 µg QEs/mg) and radical scavenging activity of the acetone extract (at a concentration of 100 µg/mL) was higher than other extracts prepared from the A. biennis. The antioxidant activity of tiliroside (the main compound of ABA-E) was investigated by different methods, including FRAP analysis (Ndhlala et al 2013, Li et al 2017, Soltani et al 2023. According to the study of Li et al (2017), the IC50 value of tiliroside in the FRAP analysis was found to be 246.8 ± 19.3 μg/mL (550.5 ± 42.9 μM).…”

Section: Discussionmentioning

confidence: 99%

Quantitative HPLC analysis and isolation of tiliroside from Alcea biennis Winterl and determination of total flavonoid content and biological activities

Sener,

Subaş,

Kanbolat

et al. 2024

Trakya University Journal of Natural Sciences

View full text Add to dashboard Cite

Natural products are being investigated to obtain potential agents that could be used to prevent and ameliorate obesity and oxidative stress, which are two important factors that cause many metabolic disorders in the body. This study aimed to isolate and quantify the major compounds, to determine total flavonoid content, and to evaluate biological activities (antioxidant and pancreatic lipase (PL) inhibitory activity) of the methanol extract and the chloroform, ethyl acetate and the remaining aqueous subextracts of Alcea biennis Winterl. The major compound isolated from the ethyl acetate subextract was determined as tiliroside (kaempferol 3-O-β-(6″-O-trans-p-coumaroyl-glucopyranoside) using 1D-NMR and 2D-NMR spectral methods. The quantification of tiliroside in the extracts was specified by the newly validated high-performance liquid chromatography (HPLC) method. Ferric-reducing antioxidant power (FRAP) assay was performed to determine the antioxidant activity and the total flavonoid content was determined. The ethyl acetate subextract was found to have the highest tiliroside (75.4263 mg/g) and total flavonoid content (189 mg QE/g) and showed the highest FRAP value (259 ± 5.1316 µM TE/g). The methanol extract had a higher PL inhibitory activity (IC50 = 100.891 ± 5.160 µg/mL) compared to the other extracts. Tiliroside exhibited higher activity (IC50 = 86.203 ± 6.323 µg/mL) than the extracts. It has been demonstrated that A. biennis may be a promising plant for treatment of obesity, and further studies should be conducted to evaluate it as a therapeutic agent.

show abstract

“…Tricetin 4 -O-β-glucopyranoside (IC 50 = 1.17 mg/mL) and Tricetin (IC 50 = 0.43 mg/mL) both exhibited α-amylase inhibitory activities comparable to acarbose (IC 50 = 0.03mg/mL). In addition, a new α-glucosidase and α-amylase inhibitor flavonoid named hypolaetin 8-O-β-D-galactopyranoside (Figure 5e) was isolated from the leaves of Thymelaea tartonraira [140]. Yang et al [135] separated luteolin (Figure 5f) from Taraxacum mongolicum, which inhibits the α-amylase enzyme (IC50 = 42.33 μg/mL).…”

Section: Flavonoidsmentioning

confidence: 99%

New Insights into the Latest Advancement in α-Amylase Inhibitors of Plant Origin with Anti-Diabetic Effects

Kashtoh

Baek

2023

Plants

View full text Add to dashboard Cite

The rising predominance of type 2 diabetes, combined with the poor medical effects seen with commercially available anti-diabetic medications, has motivated the development of innovative treatment approaches for regulating postprandial glucose levels. Natural carbohydrate digestion enzyme inhibitors might be a viable option for blocking dietary carbohydrate absorption with fewer side effects than manufactured medicines. Alpha-amylase is a metalloenzyme that facilitates digestion by breaking down polysaccharides into smaller molecules such as maltose and maltotriose. It also contributes to elevated blood glucose levels and postprandial hyperglycemia. As a result, scientists are being urged to target α-amylase and create inhibitors that can slow down the release of glucose from carbohydrate chains and prolong its absorption, thereby resulting in lower postprandial plasma glucose levels. Natural α-amylase inhibitors derived from plants have gained popularity as safe and cost-effective alternatives. The bioactive components responsible for the inhibitory actions of various plant extracts have been identified through phytochemical research, paving the way for further development and application. The majority of the findings, however, are based on in vitro investigations. Only a few animal experiments and very few human investigations have confirmed these findings. Despite some promising results, additional investigation is needed to develop feasible anti-diabetic drugs based on plant-derived pancreatic α-amylase inhibitors. This review summarizes the most recent findings from research on plant-derived pancreatic α-amylase inhibitors, including plant extracts and plant-derived bioactive compounds. Furthermore, it offers insights into the structural aspects of the crucial therapeutic target, α-amylases, in addition to their interactions with inhibitors.

show abstract

New Specific α‐Glucosidase Inhibitor Flavonoid from Thymelaea tartonraira Leaves: Structure Elucidation, Biological and Molecular Docking Studies

Cited by 12 publications

References 44 publications

HPLC-HESI-MS/MS Analysis of Phenolic Compounds from Cynoglossum tubiflorus Leaf Extracts: An Assessment of Their Cytotoxic, Antioxidant, and Antibacterial Properties

HPLC-HESI-MS/MS Analysis of Phenolic Compounds from Cynoglossum tubiflorus Leaf Extracts: An Assessment of Their Cytotoxic, Antioxidant, and Antibacterial Properties

Quantitative HPLC analysis and isolation of tiliroside from Alcea biennis Winterl and determination of total flavonoid content and biological activities

New Insights into the Latest Advancement in α-Amylase Inhibitors of Plant Origin with Anti-Diabetic Effects

Contact Info

Product

Resources

About