New Spectrophotometric Determination of Cefdinir by Batch Method and Cloud Point Extraction

Abstract

In this study, new spectrophotometric methods were developed for estimation of cefdinir (CFD); the research consists of two methods. The first method depends on the conversion of function group amine in cefdinir to diazonium salt and then combined with the 2, 5-Dimethylphenol (2, 5-DMP) reagent in the alkaline medium. The formed azo dye has a purple colour with absorption intensity at λmax 510 nm. Concentration range was obeyed Beer's law at (1-50 μg / ml), correlation coefficient was 0.9998, molar absorptivity was 1.54 × 104 L.mol-1.cm-1 and the detection limit was 0.04 μg.ml-1. The second method is including cloud point extraction (CPE) of a trace amount of the formed azo dye in the first method followed by measuring with a UV-visible spectrophotometer. Concentration range that obeyed the Beer's law was (0.2-6 μg / ml), correlation coefficient was 0.9998, molar absorptivity was1.48×105 L.mol-1.cm-1, detection limit was 0.004 μg.ml-1, Pre-concentration factor was 25 and Distribution coefficient was 125.The proposed methods were applied and proved their compatibility for estimating of ingredient compound in pure samples and pharmaceuticals by comparing them with previous studies.
 Keyword: cefdinir, diazotization, cloud point extraction, 2, 5-Dimethylphenol.

Development of a spectrophotometric analytical approach for the measurement of cefdinir in various pharmaceuticals

“…Spectrometrically, cefdinir is oxidized with 3-methylbenzothiazolone-2-hydrazone in the existence of FeCl3 in an acidic solution, resulting in a green-colored chromogen with a maximum wavelength of 660 nm,[ 10 ] also it has been estimated spectrometrically after cloud point extraction and diazotization with 2,5-dimethylphenol,[ 11 ] and high-performance thin layer chromatography (TLC), followed by densitometry to convert cefdinir dispersal on the TLC plate into digital data. [ 12 ] Cefdinir interacts with iron in an alkaline medium forming a violet-colored complex that absorbs at λmax of 550 nm. [ 13 ]…”