, we conducted mosquito surveillance in southeastern Colorado by using ovitraps and larval sampling. The aim was to determine if there were established populations of Aedes aegypti and Ae. albopictus in the region. A single female Ae. aegypti was reared from eggs collected in La Junta, CO, but Ae. albopictus was not detected. Three other species were reared from eggs and/or larvae: Ae. epactius, Culex restuans, and Cx. pipiens. Aedes aegypti and Ae. epactius were detected for the 1st time in Otero and Baca counties, respectively, and these became new county records for Colorado. Both species were detected in very low numbers, suggesting extremely low population density or sporadic introductions into southeastern Colorado.