New status of the infrared beamlines at SSRF

Zhou, Xiaojie; Zhu, Heyuan; Zhong, Jiajia; Peng, Wei; Ji, Te; Lin, Yue-Cheng; Tang, Yuzhao; Chen, Min

doi:10.1007/s41365-019-0696-x

Cited by 20 publications

(5 citation statements)

References 36 publications

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“…Temperature control was completed by unitizing a Linkam THMS600 hostage. Real time variable temperature FTIR measurements were recorded by using a Nicolet 6700 FTIR spectrometer (Thermo Scientific, USA) at the beamline BL01B of Shanghai Synchrotron Radiation Facility (SSRF) in China . IR spectra were collected every 12 s with 32 scans under the transmission mode at a resolution of 4 cm –1 within the mid-infrared range of 500–4000 cm –1 from 30 to 200 °C, higher than PBF’s melting point ( T m ) of ca.…”

Section: Methodsmentioning

confidence: 99%

Intermolecular ═C–H···O═C Hydrogen Bonding and Conformational Preference in Crystalline Poly(butylene 2,5-furandicarboxylate)

Shao

Long

et al. 2023

Macromolecules

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Besides maximizing packing efficiency, molecular interactions also play an indispensable role upon conformational ordering for crystallization of long-chain polymers. Herein, structural evolution upon heating of biobased poly(butylene 2,5-furandicarboxylate) (PBF) is followed by temperature-dependent infrared (IR) spectroscopy and wide-angle X-ray diffraction (WAXD) to elucidate the molecular interactions and conformational preference in its crystalline state. By estimating the group distances according to atomic coordinates of its crystal structure, the stronger intermolecular interaction than the van der Waals force is speculated to exist in crystalline PBF, which is fully confirmed by the changes of IR absorptions associated with C–H and CO groups upon heating as an abnormal C–H···OC hydrogen bonding. The combination of temperature-dependent C–H band position and crystal lattice expanding supports a good thermostability of this interaction up to melting, fully defining its nature as strong hydrogen bonding rather than the generally reported weak H···OC bonding in biodegradable polyesters. By further analyzing the confirmational ordering in its crystalline state, it is believed that such intermolecular C–H···OC hydrogen bonding stabilizes the energetically inaccessible syn–syn conformer of 2,5-furandicarboxylic moieties for PBF crystallization.

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Section: Methodsmentioning

confidence: 99%

Intermolecular ═C–H···O═C Hydrogen Bonding and Conformational Preference in Crystalline Poly(butylene 2,5-furandicarboxylate)

Shao

Long

et al. 2023

Macromolecules

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“…A Nicolet 6700 FTIR spectrometer coupled with a Nicolet Continuum infrared microscope from BL01B beamline of the National Facility for Protein Science in Shanghai (NFPS) at Shanghai Synchrotron Radiation Facility (SSRF) was used, which has a high brightness, spatial resolution and signal-to-noise ratio. 41 For each tissue slice, there were about 8–10 spectra recorded in the wavelength range of 600–4000 cm −1 with a resolution of 8 cm −1 , the aperture of the diaphragm was set to 50 μm, and the co-added scans was set to 32 to improve the signal-to-noise ratio. The air spectrum was recorded as a background and automatically subtracted.…”

Section: Methodsmentioning

confidence: 99%

Identification of hepatocellular carcinoma and paracancerous tissue based on the peak area in FTIR microspectroscopy

Peng¹,

Yin²,

Ma³

et al. 2022

Anal. Methods

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Section: Methodsmentioning

confidence: 99%

“…SR-FTIR microspectroscopy measurements of WRL68 cells were performed at the BL01B infrared beamline of the National Facility for Protein Science in Shanghai (NFPS) at the Shanghai Synchrotron Radiation Facility (SSRF) in China. 36 All spectra were acquired in transmission mode using a Nicolet 6700 FTIR spectrometer coupled with a Nicolet Continuμm infrared microscope from Thermo Fisher Scientific. The Nicolet Continuμm infrared microscope is equipped with a 10× visible objective, a 15× (numerical aperture (NA) = 0.58) IR/visible objective, and a 32× (NA = 0.65) IR/visible objective.…”

Section: Methodsmentioning

confidence: 99%

Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

et al. 2022

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The toxic effect of vincristine on hepatocytes has rarely been studied. Synchrotron radiation-based Fourier transform infrared (SR-FTIR) microspectroscopy is a novel technique for investigating drug–cell interaction systems. In this research, the biomolecular alterations in WRL68 cells induced by vincristine treatment were investigated by SR-FTIR microspectroscopy and were further analyzed by multivariate statistical analysis and semiquantitative methods, including principal component analysis (PCA), orthogonal partial least square-discriminant analysis (OPLS-DA), and the peak area ratios of several characteristic IR bands. In vincristine-treated WRL68 cells, alterations in lipid structures and the presence of more long-chain fatty acids were found. A decrease in protein α-helical content relative to β-sheet structures in vincristine-treated WRL68 cells was identified. The nucleic acid content was decreased relative to that of lipids and proteins in WRL68 cells treated with vincristine. These results provide important information about the toxic effect of vincristine on normal liver cells. This research also provides a new approach to reveal the biomolecular alterations in drug-treated hepatocytes by combining SR-FTIR with multivariate statistical analysis and semiquantitative methods.

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New status of the infrared beamlines at SSRF

Cited by 20 publications

References 36 publications

Intermolecular ═C–H···O═C Hydrogen Bonding and Conformational Preference in Crystalline Poly(butylene 2,5-furandicarboxylate)

Intermolecular ═C–H···O═C Hydrogen Bonding and Conformational Preference in Crystalline Poly(butylene 2,5-furandicarboxylate)

Identification of hepatocellular carcinoma and paracancerous tissue based on the peak area in FTIR microspectroscopy

Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

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