New Strategies for Tracing Foodstuffs: Biological Barcodes Utilising PCR-DGGE

Sheikha, Aly Farag El

doi:10.17140/aftnsoj-se-1-101

Cited by 15 publications

(5 citation statements)

References 40 publications

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“…Current needs on seafood traceability have driven relevant biotechnological breakthroughs that can have direct applications in the authentication or/and origin certification of seafood 3 , 9 , 10 . The study of microbial communities diversity associated to food products, as well as its linkage to a particular geographic origin, has already been applied in traceability issues, namely the molecular approach employing polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) 11 – 16 .…”

Section: Introductionmentioning

confidence: 99%

Bacterial communities 16S rDNA fingerprinting as a potential tracing tool for cultured seabass Dicentrarchus labrax

Pimentel

Marcelino

Ricardo

et al. 2017

Sci Rep

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Traceability of seafood has become crucial with market globalization and consumer’s awareness. The present study used PCR-DGGE and 454 pyrosequencing to assess if bacterial communities fingerprint associated to seabass (Dicentrarchus labrax) skin mucus can be used to discriminate the geographic origin of fishes cultured in three semi-intensive fish farms. PCR-DGGE and pyrosequencing results were congruent and suggested that this molecular approach has the potential to trace fish farms with a spatial resolution <500 m. Pyrosequencing results provided a detailed insight into the bacterial community composition of seabass skin mucus and revealed the existence of a core of bacterial communities within family Pseudomonadaceae and Rhodobacteraceae. This approach also allowed to recognized key OTUs that are potentially relevant to discriminate the geographic origin of the fish being surveyed. Overall, the present study increased our knowledge on farmed seabass microbiome and demonstrated that specific and unique bacterial taxa can act as natural signatures that allow us to trace fish to its respective geographic origin. Our study provides valuable clues that should be more investigated in future studies as a way to fulfill current traceability needs in the global trade of seafood.

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Section: Introductionmentioning

confidence: 99%

Bacterial communities 16S rDNA fingerprinting as a potential tracing tool for cultured seabass Dicentrarchus labrax

Pimentel

Marcelino

Ricardo

et al. 2017

Sci Rep

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“…Techniques that are varied with PCR such as qPCR, RT-PCR, PCR denaturing gradient gel electrophoresis (PCR-DGGE), and PCR-ELISA, can be used to control fungi in food [156]. Among them, PCR-DGGE has the advantage of terminal-restriction fragment length polymorphism analysis (T-RFLP), achieving satisfactory identifications for toxigenic fungi [29]. PCR-ELISA when compared to simple PCR, proved to be more sensitive.…”

Section: Molecular Techniquesmentioning

confidence: 99%

“…Also biosensors are a very useful tool for mycotoxins identification [25][26][27]. More emergent, recent and novel techniques for the detection and analysis of mycotoxins in foods can be performed by proteomic and genomic methods, molecular techniques, electronic nose [28,29] and hyperspectral imaging (HSI) [30,31].…”

Section: Introductionmentioning

confidence: 99%

Advances in Analysis and Detection of Major Mycotoxins in Foods

Agriopoulou

Stamatelopoulou

Varzakas

2020

Foods

140

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Mycotoxins are the most widely studied biological toxins, which contaminate foods at very low concentrations. This review describes the emerging extraction techniques and the current and alternatives analytical techniques and methods that have been used to successfully detect and identify important mycotoxins. Some of them have proven to be particularly effective in not only the detection of mycotoxins, but also in detecting mycotoxin-producing fungi. Chromatographic techniques such as high-performance liquid chromatography coupled with various detectors like fluorescence, diode array, UV, liquid chromatography coupled with mass spectrometry, and liquid chromatography-tandem mass spectrometry, have been powerful tools for analyzing and detecting major mycotoxins. Recent progress of the development of rapid immunoaffinity-based detection techniques such as immunoassays and biosensors, as well as emerging technologies like proteomic and genomic methods, molecular techniques, electronic nose, aggregation-induced emission dye, quantitative NMR and hyperspectral imaging for the detection of mycotoxins in foods, have also been presented.

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“…It provides the expected regulation information to the consumers for their own well being. Thus, traceability basically gives the products story identifying the batch and the raw materials used in the production [14]. The aim of this traceability system is to ensure nutritious and healthy products to the customers.…”

Section: Status Of Traceability Of Fruit Juice In Dhaka University and Student's Responsementioning

confidence: 99%

Awareness Situation Regarding Fruit Juice Adulteration among the Public University Students of Bangladesh: A Case from University of Dhaka

Rahman

2018

AJESS

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Food adulteration particularly fruit juice adulteration has been emanated a great concern for the consumers all across the country of Bangladesh as it bears some fatal diseases. Experts advocate that our future generation will be affected seriously for juice adulteration with vulnerable physical and mental growth. University of Dhaka is contemplated to be the premier public university in Bangladesh which is commonly distinguished as 'Oxford of East'. We chose Dhaka University as our research site to assess the awareness level about fruit juice adulteration among the common students. To address this fruit juice problem properly and awareness situation, this research paper aims to disclose this problem properly by creating more knowledge and information for all kinds of consumers including students of the studied area. The findings of the paper show that Students of the Dhaka University students are very much vulnerable to fruit juice adulteration. Most of the students are not well aware of fruit juice adulteration and its impacts. Students usually do not ask for hygienic conditions of the fruit juice. This paper discloses the current scenario of fruit juice adulteration in Dhaka University campus. At the end there some recommendations have been assembled about the roles of the concerned authorities to eradicate this problem from Dhaka University areas in particular and from whole Bangladesh in general.

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New Strategies for Tracing Foodstuffs: Biological Barcodes Utilising PCR-DGGE

Cited by 15 publications

References 40 publications

Bacterial communities 16S rDNA fingerprinting as a potential tracing tool for cultured seabass Dicentrarchus labrax

Bacterial communities 16S rDNA fingerprinting as a potential tracing tool for cultured seabass Dicentrarchus labrax

Advances in Analysis and Detection of Major Mycotoxins in Foods

Awareness Situation Regarding Fruit Juice Adulteration among the Public University Students of Bangladesh: A Case from University of Dhaka

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