Spiders of the genus Clubiona Latreille, 1804 from Xishuangbanna, Yunnan Province, China are studied. A total of 47 species is reported and illustrated, including 14 new species and two new synonyms. Twelve of the new species belong to four species groups: C. dengpao Yu & Li, sp. nov., C. subdidentata Yu & Li, sp. nov., C. tixing Yu & Li, sp. nov., C. xiaoci Yu & Li, sp. nov., C. xiaokong Yu & Li, sp. nov., C. yejiei Yu & Li, sp. nov., C. zhaoi Yu & Li, sp. nov. and C. zhigangi Yu & Li, sp. nov. from the C. corticalis group; C. mii Yu & Li, sp. nov. and C. subtongi Yu & Li, sp. nov. from the C. ternatensis group; C. banna Yu & Li, sp. nov. from the C. filicata group; and C. menglun Yu & Li, sp. nov. from the C. trivialis group. The remaining two new species, C. shuangsi Yu & Li, sp. nov. and C. wangchengi Yu & Li, sp. nov., are not readily assignable to any of the existing species groups. The female of C. cochlearis Yu & Li, 2019, the female of C. tiane Yu & Li, 2019, the female of C. bicornis Yu & Li, 2019, the male of C. lala Jäger & Dankittipakul, 2010 and the true female of C. suthepica Dankittipakul, 2008 are described for the first time. Two new synonyms are: C. vukomi Jäger & Dankittipakul, 2010 syn. nov. = C. circulata Zhang & Yin, 1998; C. melanothele Thorell, 1895 syn. nov. = Clubiona melanosticta Thorell, 1890. A checklist of Clubiona species from Xishuangbanna is provided. The DNA barcodes of almost all of the species were obtained for species delimitation, matching of sexes and future use.