New synthesis route of active substance d,l-HMPAO for preparation Technetium Tc99m Exametazime

Pijarowska-Kruszyna, Justyna; Karczmarczyk, Urszula; Jaroń, Antoni; Laszuk, Ewa; Radzik, Marcin; Garnuszek, Piotr; Mikołajczak, Renata

doi:10.5603/nmr.2017.0014

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…In addition, based on its favorable qualities, 99mTc is one of the most investigated radioisotopes in nuclear research areas [2]. The HMPAO ligand comprises of two diastereomeric isoforms are including d,l-HMPAO and meso-HMPAO [3]. 99m Tc-d,l-HMPAO is a well-established radiotracer in cerebral imaging and leukocytes labeling for detection of several infectious and inflammatory diseases [4,5].…”

Section: Introductionmentioning

confidence: 99%

Kit formulation of active pharmaceutical ingredient d,l-HMPAO as a brain perfusion diagnostic system

2019

Prog. Chem. Biochem. Res.

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The purpose of this research study is to prepare exametazime (d,l-HMPAO) kit for labeling with 99m-technetium radionuclide as a brain perfusion diagnostic system. Firstly, the active pharmaceutical ingredient d,l-HMPAO was prepared in two steps with the purity of 99.29 %. Its molecular structure was characterized using elemental analysis, Fourier-transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) technique. In second step, the d,l-HMPAO kit was prepared using six different formulations and labeled by technetium-99m radionuclide. The high radiochemical yield was attributed to the high amount of SnCl2 and adding phosphate buffer. The animal studies were conducted on the three-month old male Wistar rats. The results of the biodistribution studies revealed that, the mean activity in brain of all rats was above 1% ID/g. This showed the high isomerism purity of the synthesized compound (d,l-HMPAO) and optimization of the suggested formulations. K E Y W O R D S Exametazime HMPAO Kit Formulation Nuclear medicine Radiopharmaceutical Technetium-99m radionuclide Mohammadreza Davarpanah et.al Prog. Chem. Biochem. Res.

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Section: Introductionmentioning

confidence: 99%

Kit formulation of active pharmaceutical ingredient d,l-HMPAO as a brain perfusion diagnostic system

2019

Prog. Chem. Biochem. Res.

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The Oretical Basis and Experimental Study of Complex of Manganese (Ii) With Hexamethyl- Propilenamin-Oxime as Paramagnetic Contrast Agent for MR-Imaging of Malignant Tumors

Ussov

Belyanin

Филимонов

et al. 2019

Lučevaâ diagn. ter.

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Aim of the study. We attempted to obtain the Mn-HMPAO complex (Mangoxim) and to evaluate the possibility to apply as a paramagnetic contrast agent for enhancement of tumors at MRI. Materials and methods. The synthesis of HMPAO was carried out by using R. D. Neirinckx — J. Pijarowska-Kruszyna technique (2017), without separation of enantiomers. The Mn-HMPAO was obtained by mixing Manganese(II) carbonate with HMPAO, pH 6,2–6,5, 0,5 M. Also the quantum chemistry calculations were carried out on the structure of Mn-HMPAO using method of functional of density DFT rb3lyp/6–31+g(d,p) for isolated molecules and for water solutions (CPCM). MRI study of Mn-HMPAO uptake carried out in twelve laboratory mouses with transplanted Lewis sarcoma, in T1-w. SE mode, TR=500 ms, TE=15 ms, 256×256 matrix, FOV 15×15 cm, 3 mm thin slice. MRI acquired in 15–17 min and 60–90 min after intravenous injection of the Mn-HMPAO. The uptake was quantified as index of enhancement: IE=(Int. T1-w.MRI)Mn-HMPAO/(Int. T1- w.MRI)pre-contrast. Results. The N,N-Mn-dioxim is more stable than O,O-Mn-dioxim for 50,5 KCal/mol, so in the nature only N,N-Mn-dioxim exists. R1 of Mn-HMPAO was 3,35 1/(mM•s), below R1 of gadopentetate=3,96 1/(mM•s). The highest value of early IE was over the tumor (3,36±0,31) and over lungs (3,07±0,43). At 60 min the IE over tumor decreased to 1,58±0,14, remaining far over IE values over any other location. Conclusion. The Mn-HMPAO complex demonstrates paramagnetic properties for the application as the contrast MRI agent and probably will improve the selective enhancement of tumors.

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Leukocyte Labeling with Tc-99m-HMPAO: The Role of Leucocyte Numbers and Medication on the Labeling Efficacy and Image Quality

Karayel

Ocak

Tan

2023

Mirt

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New synthesis route of active substance d,l-HMPAO for preparation Technetium Tc99m Exametazime

Cited by 3 publications

References 6 publications

Kit formulation of active pharmaceutical ingredient d,l-HMPAO as a brain perfusion diagnostic system

Kit formulation of active pharmaceutical ingredient d,l-HMPAO as a brain perfusion diagnostic system

The Oretical Basis and Experimental Study of Complex of Manganese (Ii) With Hexamethyl- Propilenamin-Oxime as Paramagnetic Contrast Agent for MR-Imaging of Malignant Tumors

Leukocyte Labeling with Tc-99m-HMPAO: The Role of Leucocyte Numbers and Medication on the Labeling Efficacy and Image Quality

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