New system for nitrites and nitrates detection from natural water sources

Moldovan, Carmen; Ion, Marian; Dinulescu, Silviu; Savin, Mihaela; Brasoveanu, Costin; Firtat, Bogdan; Gärtner, M.; Lete, Cecilia; Mihaiu, Susana; Gheorghe, Marin; Gheorghe, Simona

doi:10.1109/biocas.2018.8584698

Cited by 1 publication

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“…Each method has advantages and disadvantages depending on the requirements and target elements. Many elements have been investigated, including Hg, , Cu, Ni, Pb, and Fe, with some approaches being sensitive but requiring complex preparation ,, and with others utilizing robust detection schemes and portability. , In this work, using genetic engineering, our aim was to develop a novel type of heavy-metal-sensing layer, having good regeneration capability, stability, and flexibility, due to the modification potential of the surface of the applied protein nanotube according to the target element. By describing this method, a framework is demonstrated in which there are many opportunities for element-optimized detections.…”

Section: Introductionmentioning

confidence: 99%

Sensing Layer for Ni Detection in Water Created by Immobilization of Bioengineered Flagellar Nanotubes on Gold Surfaces

Lábadi

Kalas

Saftics

et al. 2020

ACS Biomater. Sci. Eng.

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The environmental monitoring of Ni is targeted at a threshold limit value of 0.34 μM, as set by the World Health Organization. This sensitivity target can usually only be met by time-consuming and expensive laboratory measurements. There is a need for inexpensive, field-applicable methods, even if they are only used for signaling the necessity of a more accurate laboratory investigation. In this work, bioengineered, protein-based sensing layers were developed for Ni detection in water. Two bacterial Nibinding flagellin variants were fabricated using genetic engineering, and their applicability as Ni-sensitive biochip coatings was tested. Nanotubes of mutant flagellins were built by in vitro polymerization. A large surface density of the nanotubes on the sensor surface was achieved by covalent immobilization chemistry based on a dithiobis(succimidyl propionate) cross-linking method. The formation and density of the sensing layer was monitored and verified by spectroscopic ellipsometry and atomic force microscopy. Cyclic voltammetry (CV) measurements revealed a Ni sensitivity below 1 μM. It was also shown that, even after two months of storage, the used sensors can be regenerated and reused by rinsing in a 10 mM solution of ethylenediaminetetraacetic acid at room temperature.

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