New Technique, Using a Portable CO2 Incubator, for the Production of In Vitro Fertilized Egyptian Buffalo Embryos.

Kandil, Omaima M.; Abdoon, Ahmed S.; Murakami, Masao; Otoi, Takeshige; Suzuki, Takahiro

doi:10.1262/jrd.45.315

Cited by 9 publications

(4 citation statements)

References 23 publications

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“…No. of follicles According to Kandil et al [12], the quality of buffalo oocytes (COCs) was assessed. Depending on their cumulus investment and evenly granulated ooplasm, oocytes were divided into 4 groups under a stereomicroscope [90 x] as follows:…”

Section: Methodsmentioning

confidence: 99%

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Effect of Seasons on Ovarian Activity and Developmental Competence of Buffalo Oocytes and Embryos

Kandil,

Alhallag,

Shawky

et al. 2023

Egyptian Journal of Veterinary Sciences

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T HE AIMS of the current study were to investigate the effects of seasons on 1) oocyte yield, aspiration rate, and quality in buffalo. 2) Maturation rate and embryo developmental competence in buffalo. Buffalo ovaries [426] were collected during different seasons of the year [2021], cold seasons (from October to March) and hot seasons (from April to September), and the temperature humidity index (THI) was calculated, counting the number of ovarian follicles, and the aspiration rate and yield were recorded. Oocytes' quality was classified as excellent, good, fair, and denuded. Excellent and Good quality oocytes were in vitro matured (IVM) in tissue culture media -199 (TCM-199) + fetal calf serum 10% (FCS) + 10 μg/ml folliclestimulating hormone (FSH) + 50 μg/ml gentamicin. Oocytes were incubated for 22 h in 5% CO 2 and 38.5°C., cumulus-cell expansion and nuclear maturation were determined. Frozenthawed semen was used to fertilize mature oocytes, which were then incubated for 18 hours before being cultured in vitro for 7 days by synthetic oviduct fluid (SOF). Results demonstrated that the total and mean number of buffalo oocytes yield in the cold seasons was significantly (P˂0.05) higher when compared to hot seasons. Aspiration rate is not significantly affected by season. Cold seasons are characterized by an increased number and percentage of excellent and good oocytes Also, the hot seasons significantly (P<0.05) decreased cumulus-cell expansion and nuclear maturation of IVM buffalo oocytes. The transferable embryo rate was significantly (P˂0.05) higher in cold seasons when compared with hot seasons. In Conclusion, the hot seasons could impair reproduction in buffalo by decreasing the oocyte yield and quality. In vitro maturation and transferable embryo rates significantly increased during the cold seasons in Buffalo.

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Section: Methodsmentioning

confidence: 99%

“…According to the extent of cumulus-cell development, the cytoplasmic maturation of buffalo oocytes was evaluated and divided into 4 grades (G 0, GI, GII, GIII) [12].…”

Section: Effect Of Seasons On Oocyte Maturation Rates In Buffalomentioning

confidence: 99%

Effect of Seasons on Ovarian Activity and Developmental Competence of Buffalo Oocytes and Embryos

Kandil,

Alhallag,

Shawky

et al. 2023

Egyptian Journal of Veterinary Sciences

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“…The COCs were examined under a stereo microscope and rinsed for 3-times in oocyte maturation medium. According to Kandil et al [29], buffalo oocyte quality was assessed. Depending on the cumulus investment and equally granulated ooplasm, there were four groups of COCs under a stereomicroscope (90 x) as follows:…”

Section: Collection Of Oocytes and Ivmmentioning

confidence: 99%

“…(M5250, Sigma). The COCs were in vitro matured in an incubator at 38.5°C in a humidified atmosphere with 5% CO2 for 22 h. After 22 h from incubation, the cytoplasmic maturation of oocytes assessed according to the degree of cumulus expansion, according to the extent of cumulus-cell development, the cytoplasmic maturation of buffalo oocytes was evaluated and divided into 4-grades according to Kandil et al [29]: G0: Without expansion. GI: With slight expansion.…”

Section: Collection Of Oocytes and Ivmmentioning

confidence: 99%

Effect of melatonin on developmental competence, mitochondrial distribution, and intensity of fresh and vitrified/thawed in vitro matured buffalo oocytes

Kandil,

Rahman,

Ali

et al. 2024

Reprod Biol Endocrinol

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Background: In livestock breeding, oocyte cryopreservation is crucial for preserving and transferring superior genetic traits. This study was conducted to examine the additional effect of melatonin to maturation and vitrification media on the in vitro developmental capacity, mitochondrial distribution, and intensity of buffalo oocytes. The study involved obtaining ovaries from a slaughterhouse and conducting two phases. In the first phase, high-quality oocytes were incubated in a maturation medium with or without 10−9M melatonin for 22 h (at 38.5°C in 5% CO2). Matured oocytes were fertilized in vitro and cultured in SOF media for seven days. In the second phase, vitrified in vitro matured oocytes were stored in vitrified media (basic media (BM) containing a combination of cryoprotectants (20% Ethyl Glycol and 20% Dimethyl sulfoxide), with or without melatonin, and then stored in liquid nitrogen. Normal vitrified/thawed oocytes were fertilized in vitro and cultured as described. Finally, the matured oocytes from the fresh and vitrified/thawed groups, both with and without melatonin, were stained using DAPI and Mitotracker red to detect their viability (nuclear maturation), mitochondrial intensity, and distribution using a confocal microscope. The study found that adding 10−9M melatonin to the maturation media significantly increased maturation (85.47%), fertilization rate (84.21%)cleavage (89.58%), and transferable embryo (48.83%) rates compared to the group without melatonin (69.85%,79.88%, 75.55%, and 37.25% respectively). Besides that, the addition of melatonin to the vitrification media improved the recovery rate of normal oocytes (83.75%), as well as the cleavage (61.80%) and transferable embryo (27.00%) rates when compared to the vitrified TCM group (67.46%, 51.40%, and 17.00%, respectively). The diffuse mitochondrial distribution was higher in fresh with melatonin (TCM + Mel) (80%) and vitrified with melatonin (VS2 + Mel groups) (76.70%), Furthermore, within the same group, while the mitochondrial intensity was higher in the TCM + Mel group (1698.60) than other group. In conclusion, Melatonin supplementation improves the developmental competence and mitochondrial distribution in buffalo oocytes in both cases(in vitro maturation and vitrification).

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Influence of serum source on buffalo in vitro embryonic development with reference to heat inactivation

Mahmoud

Nawito

2005

Livestock Production Science

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New Technique, Using a Portable CO2 Incubator, for the Production of In Vitro Fertilized Egyptian Buffalo Embryos.

Cited by 9 publications

References 23 publications

Effect of Seasons on Ovarian Activity and Developmental Competence of Buffalo Oocytes and Embryos

Effect of Seasons on Ovarian Activity and Developmental Competence of Buffalo Oocytes and Embryos

Effect of melatonin on developmental competence, mitochondrial distribution, and intensity of fresh and vitrified/thawed in vitro matured buffalo oocytes

Influence of serum source on buffalo in vitro embryonic development with reference to heat inactivation

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