New Technologies for Isolation and Analysis of Bioactive Compounds

Gokavi, Sumangala

doi:10.1002/9780813821504.ch14

Cited by 3 publications

(2 citation statements)

References 56 publications

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“…Immunological methods such as single radial immunodiffusion assay and enzyme-linked immunosorbent assay (ELISA) are now considered more suitable for LF measurement because they can discriminate the tertiary structures of proteins and thus differentiate intact from denatured LF, unlike the previously mentioned methods (Gokavi 2009). Further, ELISA can give quantitative measurements and is more sensitive than the immunodiffusion techniques (Bjoerck et al 1993).…”

Section: Methods For Analysis Of Lactoferrin In Breast Milkmentioning

confidence: 99%

Factors affecting lactoferrin concentration in human milk: how much do we know?

Villavicencio

Rueda

Turin

et al. 2017

Biochem. Cell Biol.

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Lactoferrin (LF) is a breast milk glycoprotein with antimicrobial and anti-inflammatory effects. Its beneficial properties in infants, especially in those born preterm, are currently being studied in clinical trials. However, the maternal and nursing infant factors that may affect the concentration of LF in breast milk are still not clear. We conducted a systematic review to investigate the factors that may affect LF concentration. We used a 2-step approach to identify the eligible studies according to inclusion/exclusion criteria and to determine which studies would be considered. We included 70 qualified articles from 29 countries with publication dates ranging from 1976 to 2015. We described the correlation between LF concentration in breast milk and lactation stage; 10 maternal factors, such as race, parity, among others; and 2 infant factors, infections and prematurity. Colostrum has the highest LF levels, but they decrease with days postpartum. No other factor has been consistently associated with LF concentration. A major limitation of the majority of the published studies is the small sample size and the different methods used to measure LF concentration. Therefore, there is a need for large, multicenter studies with standardized study design, sample collection, and LF measurement methods to identify clinically significant factors associated with LF expression in breast milk, which will help promote exclusive breastfeeding in preterm infants.

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Section: Methods For Analysis Of Lactoferrin In Breast Milkmentioning

confidence: 99%

Factors affecting lactoferrin concentration in human milk: how much do we know?

Villavicencio

Rueda

Turin

et al. 2017

Biochem. Cell Biol.

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show abstract

“…The specifi c characteristics have been used in isolation of individual proteins from whey, but the purity has proven a critical factor in assessing the biological activity of a purifi ed component. The major whey proteins can nowadays be isolated using industrial or semi-industrial scale processing techniques (Chatterton et al , 2006;Korhonen and Pihlanto, 2007;Gokawi, 2009;Vercruysse et al , 2009).…”

Section: Caseins and Whey Proteinsmentioning

confidence: 99%